Data from: Symbiont-mediated RNA interference in insects
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and...
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| creator | Whitten, Miranda M. A. Facey, Paul D. Del Sol, Ricardo Fernandez, Lorena T. Evans, Meirwyn C. Mitchell, Jacob J. Bodger, Owen G. Dyson, Paul J. |
| description | RNA interference (RNAi) methods for insects are often limited by problems
with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides. We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis
and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing
bacterial strains were created from the symbionts of two very diverse pest
species: a long-lived blood-sucking bug, Rhodnius prolixus, and a
short-lived globally invasive polyphagous agricultural pest, Western
Flower Thrips (Frankliniella occidentalis). When ingested, the manipulated
bacteria colonized the insects, successfully competed with the wildtype
microflora, and sustainably mediated systemic knockdown phenotypes that
were horizontally transmissible. This represents a significant advance in
the ability to deliver RNAi, potentially to a large range of non-model
insects. |
| doi_str_mv | 10.5061/dryad.sf937 |
| format | Dataset |
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with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides. We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis
and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing
bacterial strains were created from the symbionts of two very diverse pest
species: a long-lived blood-sucking bug, Rhodnius prolixus, and a
short-lived globally invasive polyphagous agricultural pest, Western
Flower Thrips (Frankliniella occidentalis). When ingested, the manipulated
bacteria colonized the insects, successfully competed with the wildtype
microflora, and sustainably mediated systemic knockdown phenotypes that
were horizontally transmissible. This represents a significant advance in
the ability to deliver RNAi, potentially to a large range of non-model
insects.</description><identifier>DOI: 10.5061/dryad.sf937</identifier><language>eng</language><publisher>Dryad</publisher><subject>biocide ; Chagas disease ; RNA interference ; symbiotic bacteria</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.5061/dryad.sf937$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Whitten, Miranda M. A.</creatorcontrib><creatorcontrib>Facey, Paul D.</creatorcontrib><creatorcontrib>Del Sol, Ricardo</creatorcontrib><creatorcontrib>Fernandez, Lorena T.</creatorcontrib><creatorcontrib>Evans, Meirwyn C.</creatorcontrib><creatorcontrib>Mitchell, Jacob J.</creatorcontrib><creatorcontrib>Bodger, Owen G.</creatorcontrib><creatorcontrib>Dyson, Paul J.</creatorcontrib><title>Data from: Symbiont-mediated RNA interference in insects</title><description>RNA interference (RNAi) methods for insects are often limited by problems
with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides. We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis
and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing
bacterial strains were created from the symbionts of two very diverse pest
species: a long-lived blood-sucking bug, Rhodnius prolixus, and a
short-lived globally invasive polyphagous agricultural pest, Western
Flower Thrips (Frankliniella occidentalis). When ingested, the manipulated
bacteria colonized the insects, successfully competed with the wildtype
microflora, and sustainably mediated systemic knockdown phenotypes that
were horizontally transmissible. This represents a significant advance in
the ability to deliver RNAi, potentially to a large range of non-model
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with double-stranded (ds) RNA delivery, which restricts reverse genetics
studies and the development of RNAi-based biocides. We therefore delegated
to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis
and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing
bacterial strains were created from the symbionts of two very diverse pest
species: a long-lived blood-sucking bug, Rhodnius prolixus, and a
short-lived globally invasive polyphagous agricultural pest, Western
Flower Thrips (Frankliniella occidentalis). When ingested, the manipulated
bacteria colonized the insects, successfully competed with the wildtype
microflora, and sustainably mediated systemic knockdown phenotypes that
were horizontally transmissible. This represents a significant advance in
the ability to deliver RNAi, potentially to a large range of non-model
insects.</abstract><pub>Dryad</pub><doi>10.5061/dryad.sf937</doi><oa>free_for_read</oa></addata></record> |
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| identifier | DOI: 10.5061/dryad.sf937 |
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| language | eng |
| recordid | cdi_datacite_primary_10_5061_dryad_sf937 |
| source | DataCite |
| subjects | biocide Chagas disease RNA interference symbiotic bacteria |
| title | Data from: Symbiont-mediated RNA interference in insects |
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