Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance
Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022-bp promoter fragment upstream of the translation...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Dataset |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Yao, Jun Shen, Zedan Zhang, Yanli Wu, Xia Wang, Jianhui Sa, Gang Zhang, Yuhong Zhang, Huilong Deng, Chen Liu, Jian Hou, Siyuan Zhang, Ying Zhang, Yinan Zhao, Nan Deng, Shurong Lin, Shanzhi Zhao, Rui Chen, Shaoliang |
| description | Plasma membrane proton pumps play a crucial role in maintaining ionic
homeostasis in salt-resistant Populus euphratica under saline conditions.
High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica
roots and leaves. We isolated a 2022-bp promoter fragment upstream of the
translational start of PeHA1 from P. euphratica. The promoter-reporter
construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating
that β-glucuronidase activities increased in root, leaf, and stem tissues
under salt stress. DNA affinity purification sequencing revealed that
PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced
transcriptional response of PeWRKY1 and its interaction with PeHA1 in P.
euphratica. PeWRKY1 binding to the PeHA1 W-box in promoter region was
verified by a yeast one-hybrid assay, electrophoretic mobility shift
assay, luciferase reporter assay, and virus-induced gene silencing.
Transgenic tobacco plants overexpressing PeWRKY1 had improved expression
of NtHA4, which has a cis-acting W-box in the regulatory region, and H+
pumping activity in both in vivo and in vitro assays. We conclude that
salt stress upregulated PeHA1 transcription due to the binding of PeWRKY1
to the W-box in the promoter region of PeHA1. Thus, we conclude that
enhanced H+ pumping activity enabled salt-stressed plants to retain Na+
homeostasis. |
| doi_str_mv | 10.5061/dryad.83ng085 |
| format | Dataset |
| fullrecord | <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_5061_dryad_83ng085</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_5061_dryad_83ng085</sourcerecordid><originalsourceid>FETCH-datacite_primary_10_5061_dryad_83ng0853</originalsourceid><addsrcrecordid>eNqVzrEKwjAUQNEsDqKO7u8HWhtKpauIUnApIohTeG1e20CbhCQF-_da6Q843eUMl7E9T-IsOfKDdBPKOE91m-TZmlWlsWM_eqDRdg6DqhGe99uLQ6W09BA6AuvMYAI5MA2UVJw4BAOkO9Q1QUuagN7WkffKaEAtwWMfvqYnN5MtWzXYe9ot3bDoenmci0hiwFoFEtapAd0keCLmR_F7FMtj-q__AEc7TJA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>dataset</recordtype></control><display><type>dataset</type><title>Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance</title><source>DataCite</source><creator>Yao, Jun ; Shen, Zedan ; Zhang, Yanli ; Wu, Xia ; Wang, Jianhui ; Sa, Gang ; Zhang, Yuhong ; Zhang, Huilong ; Deng, Chen ; Liu, Jian ; Hou, Siyuan ; Zhang, Ying ; Zhang, Yinan ; Zhao, Nan ; Deng, Shurong ; Lin, Shanzhi ; Zhao, Rui ; Chen, Shaoliang</creator><creatorcontrib>Yao, Jun ; Shen, Zedan ; Zhang, Yanli ; Wu, Xia ; Wang, Jianhui ; Sa, Gang ; Zhang, Yuhong ; Zhang, Huilong ; Deng, Chen ; Liu, Jian ; Hou, Siyuan ; Zhang, Ying ; Zhang, Yinan ; Zhao, Nan ; Deng, Shurong ; Lin, Shanzhi ; Zhao, Rui ; Chen, Shaoliang</creatorcontrib><description>Plasma membrane proton pumps play a crucial role in maintaining ionic
homeostasis in salt-resistant Populus euphratica under saline conditions.
High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica
roots and leaves. We isolated a 2022-bp promoter fragment upstream of the
translational start of PeHA1 from P. euphratica. The promoter-reporter
construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating
that β-glucuronidase activities increased in root, leaf, and stem tissues
under salt stress. DNA affinity purification sequencing revealed that
PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced
transcriptional response of PeWRKY1 and its interaction with PeHA1 in P.
euphratica. PeWRKY1 binding to the PeHA1 W-box in promoter region was
verified by a yeast one-hybrid assay, electrophoretic mobility shift
assay, luciferase reporter assay, and virus-induced gene silencing.
Transgenic tobacco plants overexpressing PeWRKY1 had improved expression
of NtHA4, which has a cis-acting W-box in the regulatory region, and H+
pumping activity in both in vivo and in vitro assays. We conclude that
salt stress upregulated PeHA1 transcription due to the binding of PeWRKY1
to the W-box in the promoter region of PeHA1. Thus, we conclude that
enhanced H+ pumping activity enabled salt-stressed plants to retain Na+
homeostasis.</description><identifier>DOI: 10.5061/dryad.83ng085</identifier><language>eng</language><publisher>Dryad</publisher><subject>DNA affinity purification sequencing ; Electrophoretic mobility shift assay ; H+-ATPase ; luciferase reporter assay ; NaCl ; non-invasive microtest technique ; PeWRKY1 ; virus-induced gene silencing ; W-box</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4934-5224 ; 0000-0001-7511-3067</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,1888</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.5061/dryad.83ng085$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Yao, Jun</creatorcontrib><creatorcontrib>Shen, Zedan</creatorcontrib><creatorcontrib>Zhang, Yanli</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Wang, Jianhui</creatorcontrib><creatorcontrib>Sa, Gang</creatorcontrib><creatorcontrib>Zhang, Yuhong</creatorcontrib><creatorcontrib>Zhang, Huilong</creatorcontrib><creatorcontrib>Deng, Chen</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Hou, Siyuan</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Zhang, Yinan</creatorcontrib><creatorcontrib>Zhao, Nan</creatorcontrib><creatorcontrib>Deng, Shurong</creatorcontrib><creatorcontrib>Lin, Shanzhi</creatorcontrib><creatorcontrib>Zhao, Rui</creatorcontrib><creatorcontrib>Chen, Shaoliang</creatorcontrib><title>Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance</title><description>Plasma membrane proton pumps play a crucial role in maintaining ionic
homeostasis in salt-resistant Populus euphratica under saline conditions.
High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica
roots and leaves. We isolated a 2022-bp promoter fragment upstream of the
translational start of PeHA1 from P. euphratica. The promoter-reporter
construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating
that β-glucuronidase activities increased in root, leaf, and stem tissues
under salt stress. DNA affinity purification sequencing revealed that
PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced
transcriptional response of PeWRKY1 and its interaction with PeHA1 in P.
euphratica. PeWRKY1 binding to the PeHA1 W-box in promoter region was
verified by a yeast one-hybrid assay, electrophoretic mobility shift
assay, luciferase reporter assay, and virus-induced gene silencing.
Transgenic tobacco plants overexpressing PeWRKY1 had improved expression
of NtHA4, which has a cis-acting W-box in the regulatory region, and H+
pumping activity in both in vivo and in vitro assays. We conclude that
salt stress upregulated PeHA1 transcription due to the binding of PeWRKY1
to the W-box in the promoter region of PeHA1. Thus, we conclude that
enhanced H+ pumping activity enabled salt-stressed plants to retain Na+
homeostasis.</description><subject>DNA affinity purification sequencing</subject><subject>Electrophoretic mobility shift assay</subject><subject>H+-ATPase</subject><subject>luciferase reporter assay</subject><subject>NaCl</subject><subject>non-invasive microtest technique</subject><subject>PeWRKY1</subject><subject>virus-induced gene silencing</subject><subject>W-box</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2020</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNqVzrEKwjAUQNEsDqKO7u8HWhtKpauIUnApIohTeG1e20CbhCQF-_da6Q843eUMl7E9T-IsOfKDdBPKOE91m-TZmlWlsWM_eqDRdg6DqhGe99uLQ6W09BA6AuvMYAI5MA2UVJw4BAOkO9Q1QUuagN7WkffKaEAtwWMfvqYnN5MtWzXYe9ot3bDoenmci0hiwFoFEtapAd0keCLmR_F7FMtj-q__AEc7TJA</recordid><startdate>20201112</startdate><enddate>20201112</enddate><creator>Yao, Jun</creator><creator>Shen, Zedan</creator><creator>Zhang, Yanli</creator><creator>Wu, Xia</creator><creator>Wang, Jianhui</creator><creator>Sa, Gang</creator><creator>Zhang, Yuhong</creator><creator>Zhang, Huilong</creator><creator>Deng, Chen</creator><creator>Liu, Jian</creator><creator>Hou, Siyuan</creator><creator>Zhang, Ying</creator><creator>Zhang, Yinan</creator><creator>Zhao, Nan</creator><creator>Deng, Shurong</creator><creator>Lin, Shanzhi</creator><creator>Zhao, Rui</creator><creator>Chen, Shaoliang</creator><general>Dryad</general><scope>DYCCY</scope><scope>PQ8</scope><orcidid>https://orcid.org/0000-0002-4934-5224</orcidid><orcidid>https://orcid.org/0000-0001-7511-3067</orcidid></search><sort><creationdate>20201112</creationdate><title>Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance</title><author>Yao, Jun ; Shen, Zedan ; Zhang, Yanli ; Wu, Xia ; Wang, Jianhui ; Sa, Gang ; Zhang, Yuhong ; Zhang, Huilong ; Deng, Chen ; Liu, Jian ; Hou, Siyuan ; Zhang, Ying ; Zhang, Yinan ; Zhao, Nan ; Deng, Shurong ; Lin, Shanzhi ; Zhao, Rui ; Chen, Shaoliang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_5061_dryad_83ng0853</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2020</creationdate><topic>DNA affinity purification sequencing</topic><topic>Electrophoretic mobility shift assay</topic><topic>H+-ATPase</topic><topic>luciferase reporter assay</topic><topic>NaCl</topic><topic>non-invasive microtest technique</topic><topic>PeWRKY1</topic><topic>virus-induced gene silencing</topic><topic>W-box</topic><toplevel>online_resources</toplevel><creatorcontrib>Yao, Jun</creatorcontrib><creatorcontrib>Shen, Zedan</creatorcontrib><creatorcontrib>Zhang, Yanli</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Wang, Jianhui</creatorcontrib><creatorcontrib>Sa, Gang</creatorcontrib><creatorcontrib>Zhang, Yuhong</creatorcontrib><creatorcontrib>Zhang, Huilong</creatorcontrib><creatorcontrib>Deng, Chen</creatorcontrib><creatorcontrib>Liu, Jian</creatorcontrib><creatorcontrib>Hou, Siyuan</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Zhang, Yinan</creatorcontrib><creatorcontrib>Zhao, Nan</creatorcontrib><creatorcontrib>Deng, Shurong</creatorcontrib><creatorcontrib>Lin, Shanzhi</creatorcontrib><creatorcontrib>Zhao, Rui</creatorcontrib><creatorcontrib>Chen, Shaoliang</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Yao, Jun</au><au>Shen, Zedan</au><au>Zhang, Yanli</au><au>Wu, Xia</au><au>Wang, Jianhui</au><au>Sa, Gang</au><au>Zhang, Yuhong</au><au>Zhang, Huilong</au><au>Deng, Chen</au><au>Liu, Jian</au><au>Hou, Siyuan</au><au>Zhang, Ying</au><au>Zhang, Yinan</au><au>Zhao, Nan</au><au>Deng, Shurong</au><au>Lin, Shanzhi</au><au>Zhao, Rui</au><au>Chen, Shaoliang</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance</title><date>2020-11-12</date><risdate>2020</risdate><abstract>Plasma membrane proton pumps play a crucial role in maintaining ionic
homeostasis in salt-resistant Populus euphratica under saline conditions.
High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica
roots and leaves. We isolated a 2022-bp promoter fragment upstream of the
translational start of PeHA1 from P. euphratica. The promoter-reporter
construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating
that β-glucuronidase activities increased in root, leaf, and stem tissues
under salt stress. DNA affinity purification sequencing revealed that
PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced
transcriptional response of PeWRKY1 and its interaction with PeHA1 in P.
euphratica. PeWRKY1 binding to the PeHA1 W-box in promoter region was
verified by a yeast one-hybrid assay, electrophoretic mobility shift
assay, luciferase reporter assay, and virus-induced gene silencing.
Transgenic tobacco plants overexpressing PeWRKY1 had improved expression
of NtHA4, which has a cis-acting W-box in the regulatory region, and H+
pumping activity in both in vivo and in vitro assays. We conclude that
salt stress upregulated PeHA1 transcription due to the binding of PeWRKY1
to the W-box in the promoter region of PeHA1. Thus, we conclude that
enhanced H+ pumping activity enabled salt-stressed plants to retain Na+
homeostasis.</abstract><pub>Dryad</pub><doi>10.5061/dryad.83ng085</doi><orcidid>https://orcid.org/0000-0002-4934-5224</orcidid><orcidid>https://orcid.org/0000-0001-7511-3067</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | DOI: 10.5061/dryad.83ng085 |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_datacite_primary_10_5061_dryad_83ng085 |
| source | DataCite |
| subjects | DNA affinity purification sequencing Electrophoretic mobility shift assay H+-ATPase luciferase reporter assay NaCl non-invasive microtest technique PeWRKY1 virus-induced gene silencing W-box |
| title | Populus euphratica WRKY1 binds the promoter of PeHA1 to enhance gene expression and salt tolerance |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T19%3A42%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-datacite_PQ8&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.au=Yao,%20Jun&rft.date=2020-11-12&rft_id=info:doi/10.5061/dryad.83ng085&rft_dat=%3Cdatacite_PQ8%3E10_5061_dryad_83ng085%3C/datacite_PQ8%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |