Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores
Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an effective monitoring approach for elusive, wide-ranging species at low densities. However, few studies have attempted to maximize sampling efficiency. We present a model for combining sample accumulation and DNA degradation t...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Dataset |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Lonsinger, Robert C. Gese, Eric M. Dempsey, Steven J. Kluever, Bryan M. Waits, Lisette P. Johnson, Timothy R. |
| description | Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an
effective monitoring approach for elusive, wide-ranging species at low
densities. However, few studies have attempted to maximize sampling
efficiency. We present a model for combining sample accumulation and DNA
degradation to identify the most efficient (i.e. minimal cost per
successful sample) NDS temporal design for capture–recapture analyses. We
use scat accumulation and faecal DNA degradation rates for two sympatric
carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across
two seasons (summer and winter) in Utah, USA, to demonstrate
implementation of this approach. We estimated scat accumulation rates by
clearing and surveying transects for scats. We evaluated mitochondrial
(mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA
samples under natural field conditions for 20 fresh scats/species/season
from |
| doi_str_mv | 10.5061/dryad.23k27 |
| format | Dataset |
| fullrecord | <record><control><sourceid>datacite_PQ8</sourceid><recordid>TN_cdi_datacite_primary_10_5061_dryad_23k27</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_5061_dryad_23k27</sourcerecordid><originalsourceid>FETCH-datacite_primary_10_5061_dryad_23k273</originalsourceid><addsrcrecordid>eNqVj71KBEEQhCcxEDXyBTo_PHfvOA_M1FOMjMyHYqZ3adzpWXrmFtbUF_d-fAGjgqL4is-527ZZbpqH9j7ajLhcrb9W20v3s0MFdZbTIz1jgAbRngrSODAhhH3aD6iSlaCRdh9PFLk3xHNnqFyoZspjlSTfTJpVdEKRialn5SrhTDtic0dlTiOqHdoAU5mycbl2Fx2Gwjd_eeUWb6-fL-93hxcEqexHkwSbfdv4o4I_KfiTwvp_61-eH1hO</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>dataset</recordtype></control><display><type>dataset</type><title>Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores</title><source>DataCite</source><creator>Lonsinger, Robert C. ; Gese, Eric M. ; Dempsey, Steven J. ; Kluever, Bryan M. ; Waits, Lisette P. ; Johnson, Timothy R.</creator><creatorcontrib>Lonsinger, Robert C. ; Gese, Eric M. ; Dempsey, Steven J. ; Kluever, Bryan M. ; Waits, Lisette P. ; Johnson, Timothy R.</creatorcontrib><description>Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an
effective monitoring approach for elusive, wide-ranging species at low
densities. However, few studies have attempted to maximize sampling
efficiency. We present a model for combining sample accumulation and DNA
degradation to identify the most efficient (i.e. minimal cost per
successful sample) NDS temporal design for capture–recapture analyses. We
use scat accumulation and faecal DNA degradation rates for two sympatric
carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across
two seasons (summer and winter) in Utah, USA, to demonstrate
implementation of this approach. We estimated scat accumulation rates by
clearing and surveying transects for scats. We evaluated mitochondrial
(mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA
samples under natural field conditions for 20 fresh scats/species/season
from <1–112 days. Mean accumulation rates were nearly three times
greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day)
across seasons. Across species and seasons, mtDNA amplification success
was ≥95% through day 21. Fox nDNA amplification success was ≥70% through
day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in
winter, but declined to <50% by day 7 in summer. We identified a
common temporal sampling frame of approximately 14 days that allowed
species to be monitored simultaneously, further reducing time, survey
effort and costs. Our results suggest that when conducting repeated
surveys for capture–recapture analyses, overall cost-efficiency for NDS
may be improved with a temporal design that balances field and laboratory
costs along with deposition and degradation rates.</description><identifier>DOI: 10.5061/dryad.23k27</identifier><language>eng</language><publisher>Dryad</publisher><subject>Canis latrans ; DNA degradation ; genotyping error ; scat deposition ; Vulpes macrotis</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.5061/dryad.23k27$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Lonsinger, Robert C.</creatorcontrib><creatorcontrib>Gese, Eric M.</creatorcontrib><creatorcontrib>Dempsey, Steven J.</creatorcontrib><creatorcontrib>Kluever, Bryan M.</creatorcontrib><creatorcontrib>Waits, Lisette P.</creatorcontrib><creatorcontrib>Johnson, Timothy R.</creatorcontrib><title>Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores</title><description>Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an
effective monitoring approach for elusive, wide-ranging species at low
densities. However, few studies have attempted to maximize sampling
efficiency. We present a model for combining sample accumulation and DNA
degradation to identify the most efficient (i.e. minimal cost per
successful sample) NDS temporal design for capture–recapture analyses. We
use scat accumulation and faecal DNA degradation rates for two sympatric
carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across
two seasons (summer and winter) in Utah, USA, to demonstrate
implementation of this approach. We estimated scat accumulation rates by
clearing and surveying transects for scats. We evaluated mitochondrial
(mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA
samples under natural field conditions for 20 fresh scats/species/season
from <1–112 days. Mean accumulation rates were nearly three times
greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day)
across seasons. Across species and seasons, mtDNA amplification success
was ≥95% through day 21. Fox nDNA amplification success was ≥70% through
day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in
winter, but declined to <50% by day 7 in summer. We identified a
common temporal sampling frame of approximately 14 days that allowed
species to be monitored simultaneously, further reducing time, survey
effort and costs. Our results suggest that when conducting repeated
surveys for capture–recapture analyses, overall cost-efficiency for NDS
may be improved with a temporal design that balances field and laboratory
costs along with deposition and degradation rates.</description><subject>Canis latrans</subject><subject>DNA degradation</subject><subject>genotyping error</subject><subject>scat deposition</subject><subject>Vulpes macrotis</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2014</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNqVj71KBEEQhCcxEDXyBTo_PHfvOA_M1FOMjMyHYqZ3adzpWXrmFtbUF_d-fAGjgqL4is-527ZZbpqH9j7ajLhcrb9W20v3s0MFdZbTIz1jgAbRngrSODAhhH3aD6iSlaCRdh9PFLk3xHNnqFyoZspjlSTfTJpVdEKRialn5SrhTDtic0dlTiOqHdoAU5mycbl2Fx2Gwjd_eeUWb6-fL-93hxcEqexHkwSbfdv4o4I_KfiTwvp_61-eH1hO</recordid><startdate>20141204</startdate><enddate>20141204</enddate><creator>Lonsinger, Robert C.</creator><creator>Gese, Eric M.</creator><creator>Dempsey, Steven J.</creator><creator>Kluever, Bryan M.</creator><creator>Waits, Lisette P.</creator><creator>Johnson, Timothy R.</creator><general>Dryad</general><scope>DYCCY</scope><scope>PQ8</scope></search><sort><creationdate>20141204</creationdate><title>Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores</title><author>Lonsinger, Robert C. ; Gese, Eric M. ; Dempsey, Steven J. ; Kluever, Bryan M. ; Waits, Lisette P. ; Johnson, Timothy R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_5061_dryad_23k273</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Canis latrans</topic><topic>DNA degradation</topic><topic>genotyping error</topic><topic>scat deposition</topic><topic>Vulpes macrotis</topic><toplevel>online_resources</toplevel><creatorcontrib>Lonsinger, Robert C.</creatorcontrib><creatorcontrib>Gese, Eric M.</creatorcontrib><creatorcontrib>Dempsey, Steven J.</creatorcontrib><creatorcontrib>Kluever, Bryan M.</creatorcontrib><creatorcontrib>Waits, Lisette P.</creatorcontrib><creatorcontrib>Johnson, Timothy R.</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Lonsinger, Robert C.</au><au>Gese, Eric M.</au><au>Dempsey, Steven J.</au><au>Kluever, Bryan M.</au><au>Waits, Lisette P.</au><au>Johnson, Timothy R.</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores</title><date>2014-12-04</date><risdate>2014</risdate><abstract>Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an
effective monitoring approach for elusive, wide-ranging species at low
densities. However, few studies have attempted to maximize sampling
efficiency. We present a model for combining sample accumulation and DNA
degradation to identify the most efficient (i.e. minimal cost per
successful sample) NDS temporal design for capture–recapture analyses. We
use scat accumulation and faecal DNA degradation rates for two sympatric
carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across
two seasons (summer and winter) in Utah, USA, to demonstrate
implementation of this approach. We estimated scat accumulation rates by
clearing and surveying transects for scats. We evaluated mitochondrial
(mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA
samples under natural field conditions for 20 fresh scats/species/season
from <1–112 days. Mean accumulation rates were nearly three times
greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day)
across seasons. Across species and seasons, mtDNA amplification success
was ≥95% through day 21. Fox nDNA amplification success was ≥70% through
day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in
winter, but declined to <50% by day 7 in summer. We identified a
common temporal sampling frame of approximately 14 days that allowed
species to be monitored simultaneously, further reducing time, survey
effort and costs. Our results suggest that when conducting repeated
surveys for capture–recapture analyses, overall cost-efficiency for NDS
may be improved with a temporal design that balances field and laboratory
costs along with deposition and degradation rates.</abstract><pub>Dryad</pub><doi>10.5061/dryad.23k27</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | DOI: 10.5061/dryad.23k27 |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_datacite_primary_10_5061_dryad_23k27 |
| source | DataCite |
| subjects | Canis latrans DNA degradation genotyping error scat deposition Vulpes macrotis |
| title | Data from: Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T04%3A00%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-datacite_PQ8&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.au=Lonsinger,%20Robert%20C.&rft.date=2014-12-04&rft_id=info:doi/10.5061/dryad.23k27&rft_dat=%3Cdatacite_PQ8%3E10_5061_dryad_23k27%3C/datacite_PQ8%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |