Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies'

Dataset underlying the publication 'Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies'

The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines. To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISA...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Krzysica, Paulina, Verhoog, Loes, de Vries, Sonja, Smits, Coen, Savelkoul, H.F.J. (Huub), Tijhaar, E.J. (Edwin)
Format: Dataset
Sprache:eng
Schlagworte:
antibodies
capture ELISA
chicken
cytokines
FOS: Clinical medicine
FOS: Other agricultural sciences
FOS: Veterinary sciences
Immunology
interferon
interleukin
Other Agricultural and Veterinary Sciences
poultry
Time: 2018-2019
Veterinary Sciences
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Beschreibung
Zusammenfassung:The research is about optimization and validation of capture ELISA for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-y using commercially available antibodies and recombinant cytokines. To optimize antibody concentrations, checkerboard titration was performed. Then, validation tests for capture ELISAs were performed to assess lower limit of detection (LLOD), matrix effect, parallelism, and inter-assay variation.
DOI:10.4121/21494520.v1