Nickel toxicity to the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi, the diatom Thalassiosira weissflogii during laboratory experiments in 2021
In laboratory culture experiments, phytoplankton species were exposed to a range of nickel concentrations at GEOMAR Helmholtz Centre for Ocean Research Kiel. The experiments were perfomed between Feburary and August 2021. Overall, three experiments were conducted, each with a different taxonomical s...
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| creator | Xin, Xiaoke Faucher, Giulia Riebesell, Ulf |
| description | In laboratory culture experiments, phytoplankton species were exposed to a range of nickel concentrations at GEOMAR Helmholtz Centre for Ocean Research Kiel. The experiments were perfomed between Feburary and August 2021. Overall, three experiments were conducted, each with a different taxonomical species (the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi and the diatom Thalassiosira weissflogii). Cells were acclimated to experiment conditions for at least 1 week (under salinity 33, 18˚C, 12:12 light and dark cycle). Throughout the experiment cell density was recorded with BD Accuri C6 Flow Cytometer. After the experiment, nickel concentration was measured with ThermoFisher Scientific ElementXR. The study was supported by the OCEAN-ALK-ALIGN project funded by the Carbon to Sea and the Thistledown Foundation. |
| doi_str_mv | 10.1594/pangaea.964086 |
| format | Dataset |
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The experiments were perfomed between Feburary and August 2021. Overall, three experiments were conducted, each with a different taxonomical species (the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi and the diatom Thalassiosira weissflogii). Cells were acclimated to experiment conditions for at least 1 week (under salinity 33, 18˚C, 12:12 light and dark cycle). Throughout the experiment cell density was recorded with BD Accuri C6 Flow Cytometer. After the experiment, nickel concentration was measured with ThermoFisher Scientific ElementXR. The study was supported by the OCEAN-ALK-ALIGN project funded by the Carbon to Sea and the Thistledown Foundation.</description><identifier>DOI: 10.1594/pangaea.964086</identifier><language>eng</language><publisher>PANGAEA</publisher><subject>Amphidinium carterae ; Cell density ; Comment ; DAM CDRmare - RETAKE: CO2 removal by alkalinity enhancement: potential, benefits and risks (RETAKE) ; Day ; Dilution factor ; Emiliania huxleyi ; Flow cytometer, BD Biosciences, BD Accuri C6 ; Inductively coupled plasma mass spectrometer (ICP-MS), ThermoFisher Scientific, Element XR ; Laboratory experiment ; nickel ; ocean alkalinity enhancement ; Phytoplankton ; Phytoplankton, forward scatter ; Phytoplankton, red fluorescence ; Replicate ; Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare) ; Sample volume ; Sampling date/time, experiment ; Species, unique identification ; Species, unique identification (Semantic URI) ; Species, unique identification (URI) ; Thalassiosira weissflogii ; toxicity ; Treatment: nickel ; Type of study</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-9442-452X ; 0000-0001-8930-477X ; 0009-0001-2886-9205</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>780,1894</link.rule.ids><linktorsrc>$$Uhttps://commons.datacite.org/doi.org/10.1594/pangaea.964086$$EView_record_in_DataCite.org$$FView_record_in_$$GDataCite.org$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Xin, Xiaoke</creatorcontrib><creatorcontrib>Faucher, Giulia</creatorcontrib><creatorcontrib>Riebesell, Ulf</creatorcontrib><title>Nickel toxicity to the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi, the diatom Thalassiosira weissflogii during laboratory experiments in 2021</title><description>In laboratory culture experiments, phytoplankton species were exposed to a range of nickel concentrations at GEOMAR Helmholtz Centre for Ocean Research Kiel. The experiments were perfomed between Feburary and August 2021. Overall, three experiments were conducted, each with a different taxonomical species (the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi and the diatom Thalassiosira weissflogii). Cells were acclimated to experiment conditions for at least 1 week (under salinity 33, 18˚C, 12:12 light and dark cycle). Throughout the experiment cell density was recorded with BD Accuri C6 Flow Cytometer. After the experiment, nickel concentration was measured with ThermoFisher Scientific ElementXR. The study was supported by the OCEAN-ALK-ALIGN project funded by the Carbon to Sea and the Thistledown Foundation.</description><subject>Amphidinium carterae</subject><subject>Cell density</subject><subject>Comment</subject><subject>DAM CDRmare - RETAKE: CO2 removal by alkalinity enhancement: potential, benefits and risks (RETAKE)</subject><subject>Day</subject><subject>Dilution factor</subject><subject>Emiliania huxleyi</subject><subject>Flow cytometer, BD Biosciences, BD Accuri C6</subject><subject>Inductively coupled plasma mass spectrometer (ICP-MS), ThermoFisher Scientific, Element XR</subject><subject>Laboratory experiment</subject><subject>nickel</subject><subject>ocean alkalinity enhancement</subject><subject>Phytoplankton</subject><subject>Phytoplankton, forward scatter</subject><subject>Phytoplankton, red fluorescence</subject><subject>Replicate</subject><subject>Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare)</subject><subject>Sample volume</subject><subject>Sampling date/time, experiment</subject><subject>Species, unique identification</subject><subject>Species, unique identification (Semantic URI)</subject><subject>Species, unique identification (URI)</subject><subject>Thalassiosira weissflogii</subject><subject>toxicity</subject><subject>Treatment: nickel</subject><subject>Type of study</subject><fulltext>true</fulltext><rsrctype>dataset</rsrctype><creationdate>2023</creationdate><recordtype>dataset</recordtype><sourceid>PQ8</sourceid><recordid>eNqVj71OxDAQhN1QIKCl3gc4QnJ3nLgSoUNUVNdbi7OJV6x_ZDsieSjeEQN5AaodrWZG8yl127VN93Dc30f0IxI2x8O-fTxcqq83Nh8kUMLMhstSBRRL0LMPg-BIIlgInly0XH88OTCYCiWkza_RBGOCcLEh2pAITo6F0TOCnWahhTdrH5bg4GxRMGcOmRPCJ3HOg4SRGfopsR9B8D2kak0L0BwpsSNfMrCHbbvtrtXFgJLpZr1Xqnk5nZ9f73osWOeTjjWBadFdq3949cqr_3h3_w58A6q9aqk</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Xin, Xiaoke</creator><creator>Faucher, Giulia</creator><creator>Riebesell, Ulf</creator><general>PANGAEA</general><scope>DYCCY</scope><scope>PQ8</scope><orcidid>https://orcid.org/0000-0002-9442-452X</orcidid><orcidid>https://orcid.org/0000-0001-8930-477X</orcidid><orcidid>https://orcid.org/0009-0001-2886-9205</orcidid></search><sort><creationdate>2023</creationdate><title>Nickel toxicity to the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi, the diatom Thalassiosira weissflogii during laboratory experiments in 2021</title><author>Xin, Xiaoke ; Faucher, Giulia ; Riebesell, Ulf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-datacite_primary_10_1594_pangaea_9640863</frbrgroupid><rsrctype>datasets</rsrctype><prefilter>datasets</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amphidinium carterae</topic><topic>Cell density</topic><topic>Comment</topic><topic>DAM CDRmare - RETAKE: CO2 removal by alkalinity enhancement: potential, benefits and risks (RETAKE)</topic><topic>Day</topic><topic>Dilution factor</topic><topic>Emiliania huxleyi</topic><topic>Flow cytometer, BD Biosciences, BD Accuri C6</topic><topic>Inductively coupled plasma mass spectrometer (ICP-MS), ThermoFisher Scientific, Element XR</topic><topic>Laboratory experiment</topic><topic>nickel</topic><topic>ocean alkalinity enhancement</topic><topic>Phytoplankton</topic><topic>Phytoplankton, forward scatter</topic><topic>Phytoplankton, red fluorescence</topic><topic>Replicate</topic><topic>Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare)</topic><topic>Sample volume</topic><topic>Sampling date/time, experiment</topic><topic>Species, unique identification</topic><topic>Species, unique identification (Semantic URI)</topic><topic>Species, unique identification (URI)</topic><topic>Thalassiosira weissflogii</topic><topic>toxicity</topic><topic>Treatment: nickel</topic><topic>Type of study</topic><toplevel>online_resources</toplevel><creatorcontrib>Xin, Xiaoke</creatorcontrib><creatorcontrib>Faucher, Giulia</creatorcontrib><creatorcontrib>Riebesell, Ulf</creatorcontrib><collection>DataCite (Open Access)</collection><collection>DataCite</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Xin, Xiaoke</au><au>Faucher, Giulia</au><au>Riebesell, Ulf</au><format>book</format><genre>unknown</genre><ristype>DATA</ristype><title>Nickel toxicity to the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi, the diatom Thalassiosira weissflogii during laboratory experiments in 2021</title><date>2023</date><risdate>2023</risdate><abstract>In laboratory culture experiments, phytoplankton species were exposed to a range of nickel concentrations at GEOMAR Helmholtz Centre for Ocean Research Kiel. The experiments were perfomed between Feburary and August 2021. Overall, three experiments were conducted, each with a different taxonomical species (the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi and the diatom Thalassiosira weissflogii). Cells were acclimated to experiment conditions for at least 1 week (under salinity 33, 18˚C, 12:12 light and dark cycle). Throughout the experiment cell density was recorded with BD Accuri C6 Flow Cytometer. After the experiment, nickel concentration was measured with ThermoFisher Scientific ElementXR. The study was supported by the OCEAN-ALK-ALIGN project funded by the Carbon to Sea and the Thistledown Foundation.</abstract><pub>PANGAEA</pub><doi>10.1594/pangaea.964086</doi><orcidid>https://orcid.org/0000-0002-9442-452X</orcidid><orcidid>https://orcid.org/0000-0001-8930-477X</orcidid><orcidid>https://orcid.org/0009-0001-2886-9205</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | DOI: 10.1594/pangaea.964086 |
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| language | eng |
| recordid | cdi_datacite_primary_10_1594_pangaea_964086 |
| source | DataCite |
| subjects | Amphidinium carterae Cell density Comment DAM CDRmare - RETAKE: CO2 removal by alkalinity enhancement: potential, benefits and risks (RETAKE) Day Dilution factor Emiliania huxleyi Flow cytometer, BD Biosciences, BD Accuri C6 Inductively coupled plasma mass spectrometer (ICP-MS), ThermoFisher Scientific, Element XR Laboratory experiment nickel ocean alkalinity enhancement Phytoplankton Phytoplankton, forward scatter Phytoplankton, red fluorescence Replicate Research Mission of the German Marine Research Alliance (DAM): Marine carbon sinks in decarbonisation pathways (CDRmare) Sample volume Sampling date/time, experiment Species, unique identification Species, unique identification (Semantic URI) Species, unique identification (URI) Thalassiosira weissflogii toxicity Treatment: nickel Type of study |
| title | Nickel toxicity to the dinoflagellate Amphidinium carterae, the coccolithophore Emiliania huxleyi, the diatom Thalassiosira weissflogii during laboratory experiments in 2021 |
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