Identification and characterization of the zebrafish ClC-2 chloride channel orthologs
ClC-2 is a Cl − channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and Clcn2 knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which i...
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| Veröffentlicht in: | Pflügers Archiv 2015-08, Vol.467 (8), p.1769-1781 |
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| creator | Pérez-Rius, Carla Gaitán-Peñas, Héctor Estévez, Raúl Barrallo-Gimeno, Alejandro |
| description | ClC-2 is a Cl
−
channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and
Clcn2
knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish.
clcn2a
and
clcn2b
resulted from the teleost-specific whole genome duplication, while
clcn2c
arose from a gene duplication from
clcn2b
. The expression patterns in adult tissues and embryos of zebrafish
clcn2
paralogs support their subfunctionalization after the duplications, with
clcn2a
being enriched in excitable tissues and
clcn2c
in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in
Xenopus
oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies. |
| doi_str_mv | 10.1007/s00424-014-1614-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_csuc_</sourceid><recordid>TN_cdi_csuc_recercat_oai_recercat_cat_2072_356043</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1697209446</sourcerecordid><originalsourceid>FETCH-LOGICAL-c626t-87e5eb6a89dd17f2d5fb3ddc945938be52b5db7c322a0918c75dd9ca0202fe683</originalsourceid><addsrcrecordid>eNqNkUFv1DAQhS0EokvhB3BBkbhwCYzHsR0f0arQSpW40LPl2JNuqmxc7OTA_vo6SoEKCYmDbXn8vecZPcbecvjIAfSnDNBgUwNvaq7KdnrGdrwRWCNw8ZztAASvlVbtGXuV8x0AYNPiS3aGEoUyCDt2cxVomod-8G4e4lS5KVT-4JLzM6XhtBVjX80Hqk7UJdcP-VDtx32NhRtjGgKtgmmisYppPsQx3ubX7EXvxkxvHs9zdvPl4vv-sr7-9vVq__m69grVXLeaJHXKtSYErnsMsu9ECN400oi2I4mdDJ32AtGB4a3XMgTjHSBgT6oV54xvvj4v3ibylMocNrrhz2VdCBqtkAoaUTQfNs19ij8WyrM9DtnTOLqJ4pIt1xy1MoL_B6qMRjBNowr6_i_0Li5pKsOvlOISjFZP-k0x50S9vU_D0aWfloNdI7VbpLZEatdI7alo3j06L92Rwm_FrwwLgBuQy9N0S-nJ1_90fQBfnatY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1696150976</pqid></control><display><type>article</type><title>Identification and characterization of the zebrafish ClC-2 chloride channel orthologs</title><source>MEDLINE</source><source>SpringerLink Journals (MCLS)</source><source>Recercat</source><creator>Pérez-Rius, Carla ; Gaitán-Peñas, Héctor ; Estévez, Raúl ; Barrallo-Gimeno, Alejandro</creator><creatorcontrib>Pérez-Rius, Carla ; Gaitán-Peñas, Héctor ; Estévez, Raúl ; Barrallo-Gimeno, Alejandro</creatorcontrib><description>ClC-2 is a Cl
−
channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and
Clcn2
knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish.
clcn2a
and
clcn2b
resulted from the teleost-specific whole genome duplication, while
clcn2c
arose from a gene duplication from
clcn2b
. The expression patterns in adult tissues and embryos of zebrafish
clcn2
paralogs support their subfunctionalization after the duplications, with
clcn2a
being enriched in excitable tissues and
clcn2c
in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in
Xenopus
oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s00424-014-1614-z</identifier><identifier>PMID: 25236920</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amino Acid Sequence ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Canals de clorur ; Cell Biology ; Chloride channels ; Chloride Channels - chemistry ; Chloride Channels - genetics ; Chloride Channels - metabolism ; Chlorides - metabolism ; Danio rerio ; Databases, Genetic ; Gene Expression Regulation, Developmental ; Human Physiology ; Ion Channels ; Kinetics ; Membrane Potentials ; Metabolisme de proteïnes ; Molecular Medicine ; Molecular Sequence Data ; Neurosciences ; Nucleotide sequence ; Oocytes ; Peix zebra ; Phylogeny ; Protein Binding ; Protein metabolism ; Protein Transport ; Proteins - metabolism ; Receptors ; Receptors and Transporters ; RNA, Messenger - metabolism ; Seqüència de nucleòtids ; Xenopus ; Zebra danio ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish - metabolism ; Zebrafish Proteins - chemistry ; Zebrafish Proteins - genetics ; Zebrafish Proteins - metabolism</subject><ispartof>Pflügers Archiv, 2015-08, Vol.467 (8), p.1769-1781</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>(c) Springer Verlag, 2015 info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c626t-87e5eb6a89dd17f2d5fb3ddc945938be52b5db7c322a0918c75dd9ca0202fe683</citedby><cites>FETCH-LOGICAL-c626t-87e5eb6a89dd17f2d5fb3ddc945938be52b5db7c322a0918c75dd9ca0202fe683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00424-014-1614-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00424-014-1614-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,26951,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25236920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pérez-Rius, Carla</creatorcontrib><creatorcontrib>Gaitán-Peñas, Héctor</creatorcontrib><creatorcontrib>Estévez, Raúl</creatorcontrib><creatorcontrib>Barrallo-Gimeno, Alejandro</creatorcontrib><title>Identification and characterization of the zebrafish ClC-2 chloride channel orthologs</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch - Eur J Physiol</addtitle><addtitle>Pflugers Arch</addtitle><description>ClC-2 is a Cl
−
channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and
Clcn2
knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish.
clcn2a
and
clcn2b
resulted from the teleost-specific whole genome duplication, while
clcn2c
arose from a gene duplication from
clcn2b
. The expression patterns in adult tissues and embryos of zebrafish
clcn2
paralogs support their subfunctionalization after the duplications, with
clcn2a
being enriched in excitable tissues and
clcn2c
in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in
Xenopus
oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Canals de clorur</subject><subject>Cell Biology</subject><subject>Chloride channels</subject><subject>Chloride Channels - chemistry</subject><subject>Chloride Channels - genetics</subject><subject>Chloride Channels - metabolism</subject><subject>Chlorides - metabolism</subject><subject>Danio rerio</subject><subject>Databases, Genetic</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Human Physiology</subject><subject>Ion Channels</subject><subject>Kinetics</subject><subject>Membrane Potentials</subject><subject>Metabolisme de proteïnes</subject><subject>Molecular Medicine</subject><subject>Molecular Sequence Data</subject><subject>Neurosciences</subject><subject>Nucleotide sequence</subject><subject>Oocytes</subject><subject>Peix zebra</subject><subject>Phylogeny</subject><subject>Protein Binding</subject><subject>Protein metabolism</subject><subject>Protein Transport</subject><subject>Proteins - metabolism</subject><subject>Receptors</subject><subject>Receptors and Transporters</subject><subject>RNA, Messenger - metabolism</subject><subject>Seqüència de nucleòtids</subject><subject>Xenopus</subject><subject>Zebra danio</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - metabolism</subject><subject>Zebrafish Proteins - chemistry</subject><subject>Zebrafish Proteins - genetics</subject><subject>Zebrafish Proteins - metabolism</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>XX2</sourceid><recordid>eNqNkUFv1DAQhS0EokvhB3BBkbhwCYzHsR0f0arQSpW40LPl2JNuqmxc7OTA_vo6SoEKCYmDbXn8vecZPcbecvjIAfSnDNBgUwNvaq7KdnrGdrwRWCNw8ZztAASvlVbtGXuV8x0AYNPiS3aGEoUyCDt2cxVomod-8G4e4lS5KVT-4JLzM6XhtBVjX80Hqk7UJdcP-VDtx32NhRtjGgKtgmmisYppPsQx3ubX7EXvxkxvHs9zdvPl4vv-sr7-9vVq__m69grVXLeaJHXKtSYErnsMsu9ECN400oi2I4mdDJ32AtGB4a3XMgTjHSBgT6oV54xvvj4v3ibylMocNrrhz2VdCBqtkAoaUTQfNs19ij8WyrM9DtnTOLqJ4pIt1xy1MoL_B6qMRjBNowr6_i_0Li5pKsOvlOISjFZP-k0x50S9vU_D0aWfloNdI7VbpLZEatdI7alo3j06L92Rwm_FrwwLgBuQy9N0S-nJ1_90fQBfnatY</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Pérez-Rius, Carla</creator><creator>Gaitán-Peñas, Héctor</creator><creator>Estévez, Raúl</creator><creator>Barrallo-Gimeno, Alejandro</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>XX2</scope></search><sort><creationdate>20150801</creationdate><title>Identification and characterization of the zebrafish ClC-2 chloride channel orthologs</title><author>Pérez-Rius, Carla ; Gaitán-Peñas, Héctor ; Estévez, Raúl ; Barrallo-Gimeno, Alejandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c626t-87e5eb6a89dd17f2d5fb3ddc945938be52b5db7c322a0918c75dd9ca0202fe683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Canals de clorur</topic><topic>Cell Biology</topic><topic>Chloride channels</topic><topic>Chloride Channels - chemistry</topic><topic>Chloride Channels - genetics</topic><topic>Chloride Channels - metabolism</topic><topic>Chlorides - metabolism</topic><topic>Danio rerio</topic><topic>Databases, Genetic</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Human Physiology</topic><topic>Ion Channels</topic><topic>Kinetics</topic><topic>Membrane Potentials</topic><topic>Metabolisme de proteïnes</topic><topic>Molecular Medicine</topic><topic>Molecular Sequence Data</topic><topic>Neurosciences</topic><topic>Nucleotide sequence</topic><topic>Oocytes</topic><topic>Peix zebra</topic><topic>Phylogeny</topic><topic>Protein Binding</topic><topic>Protein metabolism</topic><topic>Protein Transport</topic><topic>Proteins - metabolism</topic><topic>Receptors</topic><topic>Receptors and Transporters</topic><topic>RNA, Messenger - metabolism</topic><topic>Seqüència de nucleòtids</topic><topic>Xenopus</topic><topic>Zebra danio</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - metabolism</topic><topic>Zebrafish Proteins - chemistry</topic><topic>Zebrafish Proteins - genetics</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pérez-Rius, Carla</creatorcontrib><creatorcontrib>Gaitán-Peñas, Héctor</creatorcontrib><creatorcontrib>Estévez, Raúl</creatorcontrib><creatorcontrib>Barrallo-Gimeno, Alejandro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Recercat</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Rius, Carla</au><au>Gaitán-Peñas, Héctor</au><au>Estévez, Raúl</au><au>Barrallo-Gimeno, Alejandro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of the zebrafish ClC-2 chloride channel orthologs</atitle><jtitle>Pflügers Archiv</jtitle><stitle>Pflugers Arch - Eur J Physiol</stitle><addtitle>Pflugers Arch</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>467</volume><issue>8</issue><spage>1769</spage><epage>1781</epage><pages>1769-1781</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>ClC-2 is a Cl
−
channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and
Clcn2
knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish.
clcn2a
and
clcn2b
resulted from the teleost-specific whole genome duplication, while
clcn2c
arose from a gene duplication from
clcn2b
. The expression patterns in adult tissues and embryos of zebrafish
clcn2
paralogs support their subfunctionalization after the duplications, with
clcn2a
being enriched in excitable tissues and
clcn2c
in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in
Xenopus
oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25236920</pmid><doi>10.1007/s00424-014-1614-z</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-6768 |
| ispartof | Pflügers Archiv, 2015-08, Vol.467 (8), p.1769-1781 |
| issn | 0031-6768 1432-2013 |
| language | eng |
| recordid | cdi_csuc_recercat_oai_recercat_cat_2072_356043 |
| source | MEDLINE; SpringerLink Journals (MCLS); Recercat |
| subjects | Amino Acid Sequence Animals Biomedical and Life Sciences Biomedicine Canals de clorur Cell Biology Chloride channels Chloride Channels - chemistry Chloride Channels - genetics Chloride Channels - metabolism Chlorides - metabolism Danio rerio Databases, Genetic Gene Expression Regulation, Developmental Human Physiology Ion Channels Kinetics Membrane Potentials Metabolisme de proteïnes Molecular Medicine Molecular Sequence Data Neurosciences Nucleotide sequence Oocytes Peix zebra Phylogeny Protein Binding Protein metabolism Protein Transport Proteins - metabolism Receptors Receptors and Transporters RNA, Messenger - metabolism Seqüència de nucleòtids Xenopus Zebra danio Zebrafish - embryology Zebrafish - genetics Zebrafish - metabolism Zebrafish Proteins - chemistry Zebrafish Proteins - genetics Zebrafish Proteins - metabolism |
| title | Identification and characterization of the zebrafish ClC-2 chloride channel orthologs |
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