Multifunctional Enzymatically Generated Hydrogels for Chronic Wound Application
The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes and high bacterial load. In this work, multifunctional hydrogels for c...
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| Veröffentlicht in: | Biomacromolecules 2017-05, Vol.18 (5), p.1544-1555 |
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| creator | Stefanov, Ivaylo Pérez-Rafael, Sílvia Hoyo, Javier Cailloux, Jonathan Santana Pérez, Orlando O Hinojosa-Caballero, Dolores Tzanov, Tzanko |
| description | The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic cross-linking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress, and bacterial contamination. In vitro studies revealed above 90% antioxidant activity, and MPO and collagenase inhibition by up to 98 and 23%, respectively. Ex vivo studies with venous leg ulcer exudates confirmed the inhibitory capacity of the dressings against MPO and MMPs. Additionally, the hydrogels reduced the population of the most frequently encountered in nonhealing wounds bacterial strains. The stable at physiological conditions and resistant to lysozyme degradation hydrogels showed high biocompatibility with human skin fibroblasts. |
| doi_str_mv | 10.1021/acs.biomac.7b00111 |
| format | Article |
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Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic cross-linking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress, and bacterial contamination. In vitro studies revealed above 90% antioxidant activity, and MPO and collagenase inhibition by up to 98 and 23%, respectively. Ex vivo studies with venous leg ulcer exudates confirmed the inhibitory capacity of the dressings against MPO and MMPs. Additionally, the hydrogels reduced the population of the most frequently encountered in nonhealing wounds bacterial strains. The stable at physiological conditions and resistant to lysozyme degradation hydrogels showed high biocompatibility with human skin fibroblasts.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/acs.biomac.7b00111</identifier><identifier>PMID: 28421746</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Bandages ; Biocatalysis ; Cells, Cultured ; Chitosan - analogs & derivatives ; Colloids in medicine ; Col·loides en medicina ; Cross-Linking Reagents - chemistry ; Enginyeria química ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Gallic Acid - analogs & derivatives ; Humans ; Hydrogels - chemical synthesis ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Hydrogels - therapeutic use ; Matrix Metalloproteinases - metabolism ; Oxidative Stress ; Peroxidase - metabolism ; Sulfhydryl Compounds - chemistry ; Wound Healing ; Àrees temàtiques de la UPC</subject><ispartof>Biomacromolecules, 2017-05, Vol.18 (5), p.1544-1555</ispartof><rights>Copyright © 2017 American Chemical Society</rights><rights>Attribution-NonCommercial-NoDerivs 3.0 Spain info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by-nc-nd/3.0/es/">http://creativecommons.org/licenses/by-nc-nd/3.0/es/</a></rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a428t-aef501ad0407ff4351573730ced380ee1877395444a71674915df02d2cf141f13</citedby><cites>FETCH-LOGICAL-a428t-aef501ad0407ff4351573730ced380ee1877395444a71674915df02d2cf141f13</cites><orcidid>0000-0002-8568-1110 ; 0000-0001-9658-6090</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.biomac.7b00111$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.biomac.7b00111$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,26953,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28421746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stefanov, Ivaylo</creatorcontrib><creatorcontrib>Pérez-Rafael, Sílvia</creatorcontrib><creatorcontrib>Hoyo, Javier</creatorcontrib><creatorcontrib>Cailloux, Jonathan</creatorcontrib><creatorcontrib>Santana Pérez, Orlando O</creatorcontrib><creatorcontrib>Hinojosa-Caballero, Dolores</creatorcontrib><creatorcontrib>Tzanov, Tzanko</creatorcontrib><title>Multifunctional Enzymatically Generated Hydrogels for Chronic Wound Application</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic cross-linking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress, and bacterial contamination. In vitro studies revealed above 90% antioxidant activity, and MPO and collagenase inhibition by up to 98 and 23%, respectively. Ex vivo studies with venous leg ulcer exudates confirmed the inhibitory capacity of the dressings against MPO and MMPs. Additionally, the hydrogels reduced the population of the most frequently encountered in nonhealing wounds bacterial strains. The stable at physiological conditions and resistant to lysozyme degradation hydrogels showed high biocompatibility with human skin fibroblasts.</description><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bandages</subject><subject>Biocatalysis</subject><subject>Cells, Cultured</subject><subject>Chitosan - analogs & derivatives</subject><subject>Colloids in medicine</subject><subject>Col·loides en medicina</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Enginyeria química</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Gallic Acid - analogs & derivatives</subject><subject>Humans</subject><subject>Hydrogels - chemical synthesis</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Hydrogels - therapeutic use</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Oxidative Stress</subject><subject>Peroxidase - metabolism</subject><subject>Sulfhydryl Compounds - chemistry</subject><subject>Wound Healing</subject><subject>Àrees temàtiques de la UPC</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>XX2</sourceid><recordid>eNp9kD1PwzAURS0E4qPwBxhQRpYUP3_EzlhVpUUq6gJitFzHhiAnLnYylF9PSgtsDE9-lu65ejoIXQMeAyZwp00ar-vQaDMWa4wB4AidAydFzgpMjr93ngtRijN0kdI7xrikjJ-iMyIZAcGKc7R67H1Xu741XR1a7bNZ-7ltdFcb7f02m9vWRt3ZKltsqxherU-ZCzGbvsXQ1iZ7CX1bZZPNxg_AruESnTjtk706vCP0fD97mi7y5Wr-MJ0sc82I7HJtHcegK8ywcI5RDlxQQbGxFZXYWpBC0JIzxrSAQrASeOUwqYhxwMABHSHY95rUGxWtsXE4QAVd_312Q7AgikgqpRyY2z2zieGjt6lTTZ2M9V63NvRJgZSlKCSnbIiSQ30MKUXr1CbWjY5bBVjt5KtBvtrLVwf5A3Rz6O_Xja1-kR_bQ2C8D-zg99DHQXj6r_ELFw6Riw</recordid><startdate>20170508</startdate><enddate>20170508</enddate><creator>Stefanov, Ivaylo</creator><creator>Pérez-Rafael, Sílvia</creator><creator>Hoyo, Javier</creator><creator>Cailloux, Jonathan</creator><creator>Santana Pérez, Orlando O</creator><creator>Hinojosa-Caballero, Dolores</creator><creator>Tzanov, Tzanko</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>XX2</scope><orcidid>https://orcid.org/0000-0002-8568-1110</orcidid><orcidid>https://orcid.org/0000-0001-9658-6090</orcidid></search><sort><creationdate>20170508</creationdate><title>Multifunctional Enzymatically Generated Hydrogels for Chronic Wound Application</title><author>Stefanov, Ivaylo ; 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Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic cross-linking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress, and bacterial contamination. In vitro studies revealed above 90% antioxidant activity, and MPO and collagenase inhibition by up to 98 and 23%, respectively. Ex vivo studies with venous leg ulcer exudates confirmed the inhibitory capacity of the dressings against MPO and MMPs. Additionally, the hydrogels reduced the population of the most frequently encountered in nonhealing wounds bacterial strains. 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| subjects | Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Bandages Biocatalysis Cells, Cultured Chitosan - analogs & derivatives Colloids in medicine Col·loides en medicina Cross-Linking Reagents - chemistry Enginyeria química Fibroblasts - drug effects Fibroblasts - metabolism Gallic Acid - analogs & derivatives Humans Hydrogels - chemical synthesis Hydrogels - chemistry Hydrogels - pharmacology Hydrogels - therapeutic use Matrix Metalloproteinases - metabolism Oxidative Stress Peroxidase - metabolism Sulfhydryl Compounds - chemistry Wound Healing Àrees temàtiques de la UPC |
| title | Multifunctional Enzymatically Generated Hydrogels for Chronic Wound Application |
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