Two photon versus one photon fluorescence excitation in whispering gallery mode microresonators
We investigate the feasibility of both one photon and two photon fluorescence excitation using whispering gallery mode microresonators. We report the linear and non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a silica whispering gallery mode resonator (...
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| creator | Pastells, Carme Marco, M. Pilar Merino, David Loza-Álvarez, Pablo Pasquardini, Laura Lorenzo, Lunelli Pederzolli, Cecilia Daldosso, Nicola Farnesi, Daniele Berneschi, Simone Righini, Giancarlo C Quercioli, Franco Conti, Gualtiero Nunzi Soria, Silvia |
| description | We investigate the feasibility of both one photon and two photon fluorescence excitation using whispering gallery mode microresonators. We report the linear and non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a silica whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also investigate the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are dylight800, tetramethyl rhodamine isothiocyanate, rhodamine 6G and fluorescein. All measurements were performed in a modified confocal microscope. |
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Pilar ; Merino, David ; Loza-Álvarez, Pablo ; Pasquardini, Laura ; Lorenzo, Lunelli ; Pederzolli, Cecilia ; Daldosso, Nicola ; Farnesi, Daniele ; Berneschi, Simone ; Righini, Giancarlo C ; Quercioli, Franco ; Conti, Gualtiero Nunzi ; Soria, Silvia</creator><creatorcontrib>Pastells, Carme ; Marco, M. Pilar ; Merino, David ; Loza-Álvarez, Pablo ; Pasquardini, Laura ; Lorenzo, Lunelli ; Pederzolli, Cecilia ; Daldosso, Nicola ; Farnesi, Daniele ; Berneschi, Simone ; Righini, Giancarlo C ; Quercioli, Franco ; Conti, Gualtiero Nunzi ; Soria, Silvia</creatorcontrib><description>We investigate the feasibility of both one photon and two photon fluorescence excitation using whispering gallery mode microresonators. We report the linear and non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a silica whispering gallery mode resonator (WGMR). 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We report the linear and non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a silica whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also investigate the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are dylight800, tetramethyl rhodamine isothiocyanate, rhodamine 6G and fluorescein. 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| source | Elsevier ScienceDirect Journals Complete; Recercat |
| subjects | Fluorescence Fluorescència Física Àrees temàtiques de la UPC |
| title | Two photon versus one photon fluorescence excitation in whispering gallery mode microresonators |
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