Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling

Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:RSC advances 2015-01, Vol.5 (78), p.63363-63371
Hauptverfasser: Lee, So-Youn, Yun, Hyung-Mun, Perez, Roman A, Gallinetti, Sara, Ginebra, Maria-Pau, Choi, Seong-Jun, Kim, Eun-Cheol, Kim, Hae-Won
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 63371
container_issue 78
container_start_page 63363
container_title RSC advances
container_volume 5
creator Lee, So-Youn
Yun, Hyung-Mun
Perez, Roman A
Gallinetti, Sara
Ginebra, Maria-Pau
Choi, Seong-Jun
Kim, Eun-Cheol
Kim, Hae-Won
description Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological tailoring has recently been achieved on CPCs by simply modulating the sizes of the initial particles. Herein, we aim to investigate the effects of nanotopological-tailored CPCs on the odontogenic differentiation of stem cells derived from human dental pulp (HDPSCs) as well as on their implicated signal pathways. The initial adhesion of the cells was substantially higher on nano-CPCs than on micro-CPCs. A series of indications of odontogenesis, including alkaline phosphatase activity and gene expressions (dentin matrix protein-1, dentin sialophosphoprotein, osteocalcin, ostepontin, and bone sialoprotein) were significantly stimulated on the nano-CPC in comparison to the micro-CPC. Furthermore, the integrin downstream pathways of the cells, including FAK, paxillin, Akt, MAPK, and NF-κB, were highly activated on the nano-CPC with respect to those on the micro-CPCs. Collectively, the nanotopological CPCs significantly enhance the odontogenic differentiation of HDPSCs when compared to conventional micro-CPCs through the integrin-associated signaling pathways, which implies that the nanotopological CPCs may be more potent in the repair and regeneration of dentin-pulp complex tissues. Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.
doi_str_mv 10.1039/c5ra11564g
format Article
fullrecord <record><control><sourceid>proquest_csuc_</sourceid><recordid>TN_cdi_csuc_recercat_oai_recercat_cat_2072_265856</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1730047615</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-5ed4df05d107fe08a6c6a8ed903bfca4c8d3dbb36ed3ae210daec81bfcbfb2f83</originalsourceid><addsrcrecordid>eNqNkUGL1TAUhYs44DDOxr0QdyJUk7RN2-Xw0FEYFIaZdbhNbtpImtQkXfgj_M-T5xscV-KFcO8l3zkknKp6xeh7Rpvxg-oiMNaJdn5WnXPaippTMT7_a35RXab0nZYSHeOCnVe_voIPOWzBhdkqcHUG60JETcqi7L6SbQlpWyAjUbiiz4mYEElekAQdfA4zeqtIynbdHWQbPAmGLPsKnuiCgyPb7rYC4FocnEtFG8M-L8T6jHO0niQ7e3DWzy-rMwMu4eVjv6juP328O3yub75dfzlc3dSq5U2uO9StNrTTjPYG6QBCCRhQj7SZjIJWDbrR09QI1A0gZ1QDqoGVu8lM3AzNRcVOvirtSkZUGBVkGcA-LcfDac8lF93QiaJ5e9JsMfzYMWW52nT8EHgMe5Ksbyhte8G6_0DpyPp-YGNB3z2-JIaUIhq5RbtC_CkZlcdU5aG7vfqd6nWB35zgmNQf7il1uWlTmNf_YpoHjamvKQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1709177819</pqid></control><display><type>article</type><title>Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling</title><source>Royal Society Of Chemistry Journals</source><source>Recercat</source><creator>Lee, So-Youn ; Yun, Hyung-Mun ; Perez, Roman A ; Gallinetti, Sara ; Ginebra, Maria-Pau ; Choi, Seong-Jun ; Kim, Eun-Cheol ; Kim, Hae-Won</creator><creatorcontrib>Lee, So-Youn ; Yun, Hyung-Mun ; Perez, Roman A ; Gallinetti, Sara ; Ginebra, Maria-Pau ; Choi, Seong-Jun ; Kim, Eun-Cheol ; Kim, Hae-Won</creatorcontrib><description>Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological tailoring has recently been achieved on CPCs by simply modulating the sizes of the initial particles. Herein, we aim to investigate the effects of nanotopological-tailored CPCs on the odontogenic differentiation of stem cells derived from human dental pulp (HDPSCs) as well as on their implicated signal pathways. The initial adhesion of the cells was substantially higher on nano-CPCs than on micro-CPCs. A series of indications of odontogenesis, including alkaline phosphatase activity and gene expressions (dentin matrix protein-1, dentin sialophosphoprotein, osteocalcin, ostepontin, and bone sialoprotein) were significantly stimulated on the nano-CPC in comparison to the micro-CPC. Furthermore, the integrin downstream pathways of the cells, including FAK, paxillin, Akt, MAPK, and NF-κB, were highly activated on the nano-CPC with respect to those on the micro-CPCs. Collectively, the nanotopological CPCs significantly enhance the odontogenic differentiation of HDPSCs when compared to conventional micro-CPCs through the integrin-associated signaling pathways, which implies that the nanotopological CPCs may be more potent in the repair and regeneration of dentin-pulp complex tissues. Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c5ra11564g</identifier><language>eng</language><subject>Biomedical materials ; bone ; Bone cements ; Calcium phosphate ; Cements ; Ciments ossis ; delivery ; Differentiation ; Enginyeria de teixits ; Enginyeria dels materials ; expression ; Human ; Materials biomèdics ; Nanostructure ; Odontoblastic differentiation ; osteogenic differentiation ; particle-size ; pathway ; Pathways ; Regeneration ; Repair ; tissue ; Tissue engineering ; Àrees temàtiques de la UPC</subject><ispartof>RSC advances, 2015-01, Vol.5 (78), p.63363-63371</ispartof><rights>info:eu-repo/semantics/openAccess &lt;a href="http://creativecommons.org/licenses/by-nc-nd/3.0/es/"&gt;http://creativecommons.org/licenses/by-nc-nd/3.0/es/&lt;/a&gt;</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-5ed4df05d107fe08a6c6a8ed903bfca4c8d3dbb36ed3ae210daec81bfcbfb2f83</citedby><cites>FETCH-LOGICAL-c423t-5ed4df05d107fe08a6c6a8ed903bfca4c8d3dbb36ed3ae210daec81bfcbfb2f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,26974,27924,27925</link.rule.ids></links><search><creatorcontrib>Lee, So-Youn</creatorcontrib><creatorcontrib>Yun, Hyung-Mun</creatorcontrib><creatorcontrib>Perez, Roman A</creatorcontrib><creatorcontrib>Gallinetti, Sara</creatorcontrib><creatorcontrib>Ginebra, Maria-Pau</creatorcontrib><creatorcontrib>Choi, Seong-Jun</creatorcontrib><creatorcontrib>Kim, Eun-Cheol</creatorcontrib><creatorcontrib>Kim, Hae-Won</creatorcontrib><title>Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling</title><title>RSC advances</title><description>Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological tailoring has recently been achieved on CPCs by simply modulating the sizes of the initial particles. Herein, we aim to investigate the effects of nanotopological-tailored CPCs on the odontogenic differentiation of stem cells derived from human dental pulp (HDPSCs) as well as on their implicated signal pathways. The initial adhesion of the cells was substantially higher on nano-CPCs than on micro-CPCs. A series of indications of odontogenesis, including alkaline phosphatase activity and gene expressions (dentin matrix protein-1, dentin sialophosphoprotein, osteocalcin, ostepontin, and bone sialoprotein) were significantly stimulated on the nano-CPC in comparison to the micro-CPC. Furthermore, the integrin downstream pathways of the cells, including FAK, paxillin, Akt, MAPK, and NF-κB, were highly activated on the nano-CPC with respect to those on the micro-CPCs. Collectively, the nanotopological CPCs significantly enhance the odontogenic differentiation of HDPSCs when compared to conventional micro-CPCs through the integrin-associated signaling pathways, which implies that the nanotopological CPCs may be more potent in the repair and regeneration of dentin-pulp complex tissues. Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.</description><subject>Biomedical materials</subject><subject>bone</subject><subject>Bone cements</subject><subject>Calcium phosphate</subject><subject>Cements</subject><subject>Ciments ossis</subject><subject>delivery</subject><subject>Differentiation</subject><subject>Enginyeria de teixits</subject><subject>Enginyeria dels materials</subject><subject>expression</subject><subject>Human</subject><subject>Materials biomèdics</subject><subject>Nanostructure</subject><subject>Odontoblastic differentiation</subject><subject>osteogenic differentiation</subject><subject>particle-size</subject><subject>pathway</subject><subject>Pathways</subject><subject>Regeneration</subject><subject>Repair</subject><subject>tissue</subject><subject>Tissue engineering</subject><subject>Àrees temàtiques de la UPC</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>XX2</sourceid><recordid>eNqNkUGL1TAUhYs44DDOxr0QdyJUk7RN2-Xw0FEYFIaZdbhNbtpImtQkXfgj_M-T5xscV-KFcO8l3zkknKp6xeh7Rpvxg-oiMNaJdn5WnXPaippTMT7_a35RXab0nZYSHeOCnVe_voIPOWzBhdkqcHUG60JETcqi7L6SbQlpWyAjUbiiz4mYEElekAQdfA4zeqtIynbdHWQbPAmGLPsKnuiCgyPb7rYC4FocnEtFG8M-L8T6jHO0niQ7e3DWzy-rMwMu4eVjv6juP328O3yub75dfzlc3dSq5U2uO9StNrTTjPYG6QBCCRhQj7SZjIJWDbrR09QI1A0gZ1QDqoGVu8lM3AzNRcVOvirtSkZUGBVkGcA-LcfDac8lF93QiaJ5e9JsMfzYMWW52nT8EHgMe5Ksbyhte8G6_0DpyPp-YGNB3z2-JIaUIhq5RbtC_CkZlcdU5aG7vfqd6nWB35zgmNQf7il1uWlTmNf_YpoHjamvKQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Lee, So-Youn</creator><creator>Yun, Hyung-Mun</creator><creator>Perez, Roman A</creator><creator>Gallinetti, Sara</creator><creator>Ginebra, Maria-Pau</creator><creator>Choi, Seong-Jun</creator><creator>Kim, Eun-Cheol</creator><creator>Kim, Hae-Won</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7QQ</scope><scope>7SR</scope><scope>8BQ</scope><scope>JG9</scope><scope>XX2</scope></search><sort><creationdate>20150101</creationdate><title>Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling</title><author>Lee, So-Youn ; Yun, Hyung-Mun ; Perez, Roman A ; Gallinetti, Sara ; Ginebra, Maria-Pau ; Choi, Seong-Jun ; Kim, Eun-Cheol ; Kim, Hae-Won</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-5ed4df05d107fe08a6c6a8ed903bfca4c8d3dbb36ed3ae210daec81bfcbfb2f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomedical materials</topic><topic>bone</topic><topic>Bone cements</topic><topic>Calcium phosphate</topic><topic>Cements</topic><topic>Ciments ossis</topic><topic>delivery</topic><topic>Differentiation</topic><topic>Enginyeria de teixits</topic><topic>Enginyeria dels materials</topic><topic>expression</topic><topic>Human</topic><topic>Materials biomèdics</topic><topic>Nanostructure</topic><topic>Odontoblastic differentiation</topic><topic>osteogenic differentiation</topic><topic>particle-size</topic><topic>pathway</topic><topic>Pathways</topic><topic>Regeneration</topic><topic>Repair</topic><topic>tissue</topic><topic>Tissue engineering</topic><topic>Àrees temàtiques de la UPC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, So-Youn</creatorcontrib><creatorcontrib>Yun, Hyung-Mun</creatorcontrib><creatorcontrib>Perez, Roman A</creatorcontrib><creatorcontrib>Gallinetti, Sara</creatorcontrib><creatorcontrib>Ginebra, Maria-Pau</creatorcontrib><creatorcontrib>Choi, Seong-Jun</creatorcontrib><creatorcontrib>Kim, Eun-Cheol</creatorcontrib><creatorcontrib>Kim, Hae-Won</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Recercat</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, So-Youn</au><au>Yun, Hyung-Mun</au><au>Perez, Roman A</au><au>Gallinetti, Sara</au><au>Ginebra, Maria-Pau</au><au>Choi, Seong-Jun</au><au>Kim, Eun-Cheol</au><au>Kim, Hae-Won</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>78</issue><spage>63363</spage><epage>63371</epage><pages>63363-63371</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Calcium phosphate cements (CPCs) are a unique class of inorganic injectables attractive for the repair and regeneration of hard tissues. Tailoring the crystallite properties of CPC, particularly to represent nanotopological features, is favorable for stimulating biological reactions. Nanotopological tailoring has recently been achieved on CPCs by simply modulating the sizes of the initial particles. Herein, we aim to investigate the effects of nanotopological-tailored CPCs on the odontogenic differentiation of stem cells derived from human dental pulp (HDPSCs) as well as on their implicated signal pathways. The initial adhesion of the cells was substantially higher on nano-CPCs than on micro-CPCs. A series of indications of odontogenesis, including alkaline phosphatase activity and gene expressions (dentin matrix protein-1, dentin sialophosphoprotein, osteocalcin, ostepontin, and bone sialoprotein) were significantly stimulated on the nano-CPC in comparison to the micro-CPC. Furthermore, the integrin downstream pathways of the cells, including FAK, paxillin, Akt, MAPK, and NF-κB, were highly activated on the nano-CPC with respect to those on the micro-CPCs. Collectively, the nanotopological CPCs significantly enhance the odontogenic differentiation of HDPSCs when compared to conventional micro-CPCs through the integrin-associated signaling pathways, which implies that the nanotopological CPCs may be more potent in the repair and regeneration of dentin-pulp complex tissues. Nanotopological-tailored calcium phosphate cements stimulate odontogenesis of human dental pulp cells through integrin-mediated signaling pathways.</abstract><doi>10.1039/c5ra11564g</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2046-2069
ispartof RSC advances, 2015-01, Vol.5 (78), p.63363-63371
issn 2046-2069
2046-2069
language eng
recordid cdi_csuc_recercat_oai_recercat_cat_2072_265856
source Royal Society Of Chemistry Journals; Recercat
subjects Biomedical materials
bone
Bone cements
Calcium phosphate
Cements
Ciments ossis
delivery
Differentiation
Enginyeria de teixits
Enginyeria dels materials
expression
Human
Materials biomèdics
Nanostructure
Odontoblastic differentiation
osteogenic differentiation
particle-size
pathway
Pathways
Regeneration
Repair
tissue
Tissue engineering
Àrees temàtiques de la UPC
title Nanotopological-tailored calcium phosphate cements for the odontogenic stimulation of human dental pulp stem cells through integrin signaling
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A03%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_csuc_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nanotopological-tailored%20calcium%20phosphate%20cements%20for%20the%20odontogenic%20stimulation%20of%20human%20dental%20pulp%20stem%20cells%20through%20integrin%20signaling&rft.jtitle=RSC%20advances&rft.au=Lee,%20So-Youn&rft.date=2015-01-01&rft.volume=5&rft.issue=78&rft.spage=63363&rft.epage=63371&rft.pages=63363-63371&rft.issn=2046-2069&rft.eissn=2046-2069&rft_id=info:doi/10.1039/c5ra11564g&rft_dat=%3Cproquest_csuc_%3E1730047615%3C/proquest_csuc_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1709177819&rft_id=info:pmid/&rfr_iscdi=true