Prevalence and Concentration of Non-tuberculous Mycobacteria in Cooling Towers by Means of Quantitative PCR: A Prospective Study
There is an increasing level of interest in non-tuberculous mycobacteria (NTM) due to the increasing reported rates of diseases caused by them. Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In t...
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| Veröffentlicht in: | Current microbiology 2011-01, Vol.62 (1), p.313-319 |
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| creator | Adrados, Bárbara Julián, Esther Codony, Francesc Torrents, Eduard Luquin, Marina Morató, Jordi |
| description | There is an increasing level of interest in non-tuberculous mycobacteria (NTM) due to the increasing reported rates of diseases caused by them. Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In this study, we aimed to investigate the occurrence and levels of NTM in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. In the current study, we detected and quantified the presence of NTM by means of a rapid method in water samples taken from 53 cooling towers of an urban area (Barcelona, Spain). A genus-specific quantitative PCR (Q-PCR) assay with a quantification limit (QL) of 500 cells l⁻¹ was used. 56% (30) of samples were positive with a concentration range from 4.6 × 10³ to 1.79 × 10⁶ cells l⁻¹. In some cases (9/30), samples were positive but with levels below the QL. The colonization rate confirmed that cooling towers could be considered as a potential reservoir for NTM. This study also evaluated Q-PCR as a useful method to detect and quantify NTM in samples coming from environmental sources. |
| doi_str_mv | 10.1007/s00284-010-9706-2 |
| format | Article |
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Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In this study, we aimed to investigate the occurrence and levels of NTM in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. In the current study, we detected and quantified the presence of NTM by means of a rapid method in water samples taken from 53 cooling towers of an urban area (Barcelona, Spain). A genus-specific quantitative PCR (Q-PCR) assay with a quantification limit (QL) of 500 cells l⁻¹ was used. 56% (30) of samples were positive with a concentration range from 4.6 × 10³ to 1.79 × 10⁶ cells l⁻¹. In some cases (9/30), samples were positive but with levels below the QL. The colonization rate confirmed that cooling towers could be considered as a potential reservoir for NTM. 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Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In this study, we aimed to investigate the occurrence and levels of NTM in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. In the current study, we detected and quantified the presence of NTM by means of a rapid method in water samples taken from 53 cooling towers of an urban area (Barcelona, Spain). A genus-specific quantitative PCR (Q-PCR) assay with a quantification limit (QL) of 500 cells l⁻¹ was used. 56% (30) of samples were positive with a concentration range from 4.6 × 10³ to 1.79 × 10⁶ cells l⁻¹. In some cases (9/30), samples were positive but with levels below the QL. The colonization rate confirmed that cooling towers could be considered as a potential reservoir for NTM. This study also evaluated Q-PCR as a useful method to detect and quantify NTM in samples coming from environmental sources.</description><subject>Aerosols</subject><subject>Aigua</subject><subject>Bacteria</subject><subject>Bacterial Load - methods</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Ciències de la terra i de la vida</subject><subject>Contaminació</subject><subject>Cooling towers</subject><subject>Energies</subject><subject>Enginyeria agroalimentària</subject><subject>Genes</subject><subject>Humans</subject><subject>Infections</subject><subject>Life Sciences</subject><subject>Microbiologia</subject><subject>Microbiology</subject><subject>Mycobacteria</subject><subject>Mycobacterium - classification</subject><subject>Mycobacterium - genetics</subject><subject>Mycobacterium - isolation & purification</subject><subject>Plant diseases</subject><subject>Pollution</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prevalence</subject><subject>Prospective Studies</subject><subject>Refrigeració</subject><subject>Spain</subject><subject>Termoenergètica</subject><subject>Torres de refrigeració</subject><subject>Tuberculosis</subject><subject>Urban areas</subject><subject>Water</subject><subject>Water analysis</subject><subject>Water Microbiology</subject><subject>Water sampling</subject><subject>Àrees temàtiques de la UPC</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>XX2</sourceid><recordid>eNqFUctu1DAUtRCIDoUPYAMWG1aB60cSh1014iW1MNB2bTnO9ShVxp7aSdHs-HQcUihiAZYs29fnnPs4hDxl8IoB1K8TAFeyAAZFU0NV8HtkxaTgBTQNu09WIKQoVFWyI_IopSsAxhtgD8kRh0qCKsWKfN9EvDEDeovU-I6uQ775MZqxD54GRz8FX4xTi9FOQ5gSPTvY0Bo7YuwN7X0mhKH3W3oRvmFMtD3QMzQ-zdQvk_FjP2apG6Sb9dc39IRuYkh7tD9D5-PUHR6TB84MCZ_cnsfk8t3bi_WH4vTz-4_rk9PCykqNheusK5XKmTk6UFJZY6ToZM0dg7ZtnLB5ccuswbLMUTBohKuFVbIFXopjwhZdmyarI9rckRl1MP3dY94caq55xZSoM-flwtnHcD1hGvWuTxaHwXjMs9BKKWhEU_L_I7Nsw3kza774C3kVpuhz61oxVefOZPVHsXlcKaLT-9jvTDxoBnq2Xi_W62y9nq3XcwnPboWndofdb8YvrzOAL4CUv_wW413mf6k-X0jOBG22sU_68pwDE8ByP0ww8QPAhcNv</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Adrados, Bárbara</creator><creator>Julián, Esther</creator><creator>Codony, Francesc</creator><creator>Torrents, Eduard</creator><creator>Luquin, Marina</creator><creator>Morató, Jordi</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>XX2</scope></search><sort><creationdate>20110101</creationdate><title>Prevalence and Concentration of Non-tuberculous Mycobacteria in Cooling Towers by Means of Quantitative PCR: A Prospective Study</title><author>Adrados, Bárbara ; Julián, Esther ; Codony, Francesc ; Torrents, Eduard ; Luquin, Marina ; Morató, Jordi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-fdcf588bac2ef0848caa43d472f10bb9f3cccc2c1cae5572f0aea3f73c84b0253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aerosols</topic><topic>Aigua</topic><topic>Bacteria</topic><topic>Bacterial Load - 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Academic</collection><collection>Recercat</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adrados, Bárbara</au><au>Julián, Esther</au><au>Codony, Francesc</au><au>Torrents, Eduard</au><au>Luquin, Marina</au><au>Morató, Jordi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and Concentration of Non-tuberculous Mycobacteria in Cooling Towers by Means of Quantitative PCR: A Prospective Study</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>62</volume><issue>1</issue><spage>313</spage><epage>319</epage><pages>313-319</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>There is an increasing level of interest in non-tuberculous mycobacteria (NTM) due to the increasing reported rates of diseases caused by them. Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In this study, we aimed to investigate the occurrence and levels of NTM in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. In the current study, we detected and quantified the presence of NTM by means of a rapid method in water samples taken from 53 cooling towers of an urban area (Barcelona, Spain). A genus-specific quantitative PCR (Q-PCR) assay with a quantification limit (QL) of 500 cells l⁻¹ was used. 56% (30) of samples were positive with a concentration range from 4.6 × 10³ to 1.79 × 10⁶ cells l⁻¹. In some cases (9/30), samples were positive but with levels below the QL. The colonization rate confirmed that cooling towers could be considered as a potential reservoir for NTM. This study also evaluated Q-PCR as a useful method to detect and quantify NTM in samples coming from environmental sources.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>20640853</pmid><doi>10.1007/s00284-010-9706-2</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aerosols Aigua Bacteria Bacterial Load - methods Biomedical and Life Sciences Biotechnology Ciències de la terra i de la vida Contaminació Cooling towers Energies Enginyeria agroalimentària Genes Humans Infections Life Sciences Microbiologia Microbiology Mycobacteria Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Plant diseases Pollution Polymerase Chain Reaction - methods Prevalence Prospective Studies Refrigeració Spain Termoenergètica Torres de refrigeració Tuberculosis Urban areas Water Water analysis Water Microbiology Water sampling Àrees temàtiques de la UPC |
| title | Prevalence and Concentration of Non-tuberculous Mycobacteria in Cooling Towers by Means of Quantitative PCR: A Prospective Study |
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