Influence of fabrication parameters in cellular microarrays for stem cell studies
Lately there has been an increasing interest in the development of tools that enable the high throughput analysis of combinations of surface-immobilized signaling factors and which examine their effect on stem cell biology and differentiation. These surface-immobilized factors function as artificial...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2009-07, Vol.20 (7), p.1525-1533 |
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| creator | Rodríguez-Seguí, Santiago A. Pla-Roca, Mateu Engel, Elisabeth Planell, Josep A. Martínez, Elena Samitier, Josep |
| description | Lately there has been an increasing interest in the development of tools that enable the high throughput analysis of combinations of surface-immobilized signaling factors and which examine their effect on stem cell biology and differentiation. These surface-immobilized factors function as artificial microenvironments that can be ordered in a microarray format. These microarrays could be useful for applications such as the study of stem cell biology to get a deeper understanding of their differentiation process. Here, the evaluation of several key process parameters affecting the cellular microarray fabrication is reported in terms of its effects on the mesenchymal stem cell culture time on these microarrays. Substrate and protein solution requirements, passivation strategies and cell culture conditions are investigated. The results described in this article serve as a basis for the future development of cellular microarrays aiming to provide a deeper understanding of the stem cell differentiation process. |
| doi_str_mv | 10.1007/s10856-009-3716-2 |
| format | Article |
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These surface-immobilized factors function as artificial microenvironments that can be ordered in a microarray format. These microarrays could be useful for applications such as the study of stem cell biology to get a deeper understanding of their differentiation process. Here, the evaluation of several key process parameters affecting the cellular microarray fabrication is reported in terms of its effects on the mesenchymal stem cell culture time on these microarrays. Substrate and protein solution requirements, passivation strategies and cell culture conditions are investigated. 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Materials in medicine, 2009-07, Vol.20 (7), p.1525-1533</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><rights>Attribution-NonCommercial-NoDerivs 3.0 Spain info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by-nc-nd/3.0/es/">http://creativecommons.org/licenses/by-nc-nd/3.0/es/</a></rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-f43563b740a4d724d1fa6cc2517588dfabff7238cc5b99e108976d5d159ae84d3</citedby><cites>FETCH-LOGICAL-c473t-f43563b740a4d724d1fa6cc2517588dfabff7238cc5b99e108976d5d159ae84d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-009-3716-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10856-009-3716-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,26973,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19301106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez-Seguí, Santiago A.</creatorcontrib><creatorcontrib>Pla-Roca, Mateu</creatorcontrib><creatorcontrib>Engel, Elisabeth</creatorcontrib><creatorcontrib>Planell, Josep A.</creatorcontrib><creatorcontrib>Martínez, Elena</creatorcontrib><creatorcontrib>Samitier, Josep</creatorcontrib><title>Influence of fabrication parameters in cellular microarrays for stem cell studies</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Lately there has been an increasing interest in the development of tools that enable the high throughput analysis of combinations of surface-immobilized signaling factors and which examine their effect on stem cell biology and differentiation. These surface-immobilized factors function as artificial microenvironments that can be ordered in a microarray format. These microarrays could be useful for applications such as the study of stem cell biology to get a deeper understanding of their differentiation process. Here, the evaluation of several key process parameters affecting the cellular microarray fabrication is reported in terms of its effects on the mesenchymal stem cell culture time on these microarrays. Substrate and protein solution requirements, passivation strategies and cell culture conditions are investigated. The results described in this article serve as a basis for the future development of cellular microarrays aiming to provide a deeper understanding of the stem cell differentiation process.</description><subject>Animals</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Cèl·lules</subject><subject>DNA microarrays</subject><subject>Enginyeria dels materials</subject><subject>Equipment Design</subject><subject>Flow Cytometry - instrumentation</subject><subject>Flow Cytometry - methods</subject><subject>Glass</subject><subject>Humans</subject><subject>Materials Science</subject><subject>Microarray Analysis - 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| subjects | Animals Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Ceramics Chemistry and Materials Science Composites Cèl·lules DNA microarrays Enginyeria dels materials Equipment Design Flow Cytometry - instrumentation Flow Cytometry - methods Glass Humans Materials Science Microarray Analysis - instrumentation Microarray Analysis - methods Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microxips d'ADN Natural Materials Polymer Sciences Proteome - analysis Regenerative Medicine/Tissue Engineering Stem cells Stem Cells - cytology Stem Cells - metabolism Surfaces and Interfaces Technology Assessment, Biomedical Thin Films Àrees temàtiques de la UPC |
| title | Influence of fabrication parameters in cellular microarrays for stem cell studies |
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