Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR
The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human hea...
Gespeichert in:
| Veröffentlicht in: | Journal of virological methods 2010-09, Vol.168 (1), p.228-232 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 232 |
|---|---|
| container_issue | 1 |
| container_start_page | 228 |
| container_title | Journal of virological methods |
| container_volume | 168 |
| creator | Fittipaldi, Mariana Rodriguez, Nancy J. Pino Codony, Francesc Adrados, Bárbara Peñuela, Gustavo A. Morató, Jordi |
| description | The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human health hazard. To assess whether propidium monoazide (PMA) pre-treatment is a good approach to inhibiting DNA amplification from non-infectious viruses, bacteriophage T4 survival was measured using cell culture titration and real-time PCR with and without PMA pre-treatment. Heat (85
°C) and proteolysis methods were carried out. After these inactivation treatments, the results indicated that the PMA pre-treatment approach is not appropriate for differentiating infectious viruses. However, when a heat treatment at 110
°C was undertaken, PMA pre-treatment did allow differentiation of non-infectious from infectious viruses. In this case, effective binding of PMA to bacteriophage T4 DNA could be taken to indicate capsid damage. Therefore, PMA pre-treatment may be appropriate for assessing effective disinfection treatments and for a more reliable understanding of the factors that contribute to viral inactivation through capsid damage monitoring. The PMA-PCR approach could be useful as a rapid and inexpensive analytical tool for screening and evaluation of the efficacy of disinfectants. |
| doi_str_mv | 10.1016/j.jviromet.2010.06.011 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_csuc_</sourceid><recordid>TN_cdi_csuc_recercat_oai_recercat_cat_2072_195675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0166093410002235</els_id><sourcerecordid>807256399</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-3e1db87e537c25016e2ade7da730cd044ce49ff9a95c937b76f97ca1ad2b507b3</originalsourceid><addsrcrecordid>eNqFUcFu1DAQtRCILoVfqHxBnLKM49iOb6At0EqVQKhI3CzHnoBXSbzYSaXy9TjaLT32YNkzes_z5j1CLhhsGTD5fr_d34UUR5y3NZQmyC0w9oxsWKt0BbptnpNNAcry5s0ZeZXzHgCE4vwlOatBaC0kbMjPy5BdCmOY7BziRGNPw9SjK8WSaWfdjCnEw2_7C-ltQ8vMtX1PDykegg_LSMc4Rfs3eKQJ7VDNYUT6bff9NXnR2yHjm9N9Tn58_nS7u6puvn653n28qVyj2FxxZL5rFQquXC2KYKytR-Wt4uA8NI3DRve9tlo4zVWnZK-Vs8z6uhOgOn5O2PFflxdnEjpMzs4m2vBYrKcGVRtWllaicN4dOWWLPwvm2YzFBRwGO2FZ27QFKyTX-kmkajSwVmpZkPKkI8WcE_bmUGy16d4wMGtkZm8eIjNrZAakKZEV4sVpxNKN6P_THjIqgLcngM3ODn2ykwv5EcehbUW9av1wxGGx-y5gMtkFnBz6UJyYjY_hKS3_AFQIuL0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>749018696</pqid></control><display><type>article</type><title>Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Recercat</source><creator>Fittipaldi, Mariana ; Rodriguez, Nancy J. Pino ; Codony, Francesc ; Adrados, Bárbara ; Peñuela, Gustavo A. ; Morató, Jordi</creator><creatorcontrib>Fittipaldi, Mariana ; Rodriguez, Nancy J. Pino ; Codony, Francesc ; Adrados, Bárbara ; Peñuela, Gustavo A. ; Morató, Jordi</creatorcontrib><description>The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human health hazard. To assess whether propidium monoazide (PMA) pre-treatment is a good approach to inhibiting DNA amplification from non-infectious viruses, bacteriophage T4 survival was measured using cell culture titration and real-time PCR with and without PMA pre-treatment. Heat (85
°C) and proteolysis methods were carried out. After these inactivation treatments, the results indicated that the PMA pre-treatment approach is not appropriate for differentiating infectious viruses. However, when a heat treatment at 110
°C was undertaken, PMA pre-treatment did allow differentiation of non-infectious from infectious viruses. In this case, effective binding of PMA to bacteriophage T4 DNA could be taken to indicate capsid damage. Therefore, PMA pre-treatment may be appropriate for assessing effective disinfection treatments and for a more reliable understanding of the factors that contribute to viral inactivation through capsid damage monitoring. The PMA-PCR approach could be useful as a rapid and inexpensive analytical tool for screening and evaluation of the efficacy of disinfectants.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2010.06.011</identifier><identifier>PMID: 20599560</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Azides - metabolism ; Bacteria ; Bacteriologia ; Bacteriology ; Bacteriophage T4 ; Bacteriophage T4 - growth & development ; Bacteriophage T4 - isolation & purification ; Biological and medical sciences ; Capsids ; Ciències de la salut ; Fundamental and applied biological sciences. Psychology ; Hot Temperature ; Humans ; Intercalating Agents - metabolism ; Medicina ; Microbial Viability ; Microbiology ; PMA ; Polymerase Chain Reaction - methods ; Propidium - analogs & derivatives ; Propidium - metabolism ; Real-time PCR ; Sensitivity and Specificity ; Specimen Handling - methods ; Techniques used in virology ; Virology ; Virology - methods ; Virus ; Virus infectivity ; Viruses ; Àrees temàtiques de la UPC</subject><ispartof>Journal of virological methods, 2010-09, Vol.168 (1), p.228-232</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier B.V. All rights reserved.</rights><rights>info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-3e1db87e537c25016e2ade7da730cd044ce49ff9a95c937b76f97ca1ad2b507b3</citedby><cites>FETCH-LOGICAL-c471t-3e1db87e537c25016e2ade7da730cd044ce49ff9a95c937b76f97ca1ad2b507b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2010.06.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,26955,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23088529$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20599560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fittipaldi, Mariana</creatorcontrib><creatorcontrib>Rodriguez, Nancy J. Pino</creatorcontrib><creatorcontrib>Codony, Francesc</creatorcontrib><creatorcontrib>Adrados, Bárbara</creatorcontrib><creatorcontrib>Peñuela, Gustavo A.</creatorcontrib><creatorcontrib>Morató, Jordi</creatorcontrib><title>Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human health hazard. To assess whether propidium monoazide (PMA) pre-treatment is a good approach to inhibiting DNA amplification from non-infectious viruses, bacteriophage T4 survival was measured using cell culture titration and real-time PCR with and without PMA pre-treatment. Heat (85
°C) and proteolysis methods were carried out. After these inactivation treatments, the results indicated that the PMA pre-treatment approach is not appropriate for differentiating infectious viruses. However, when a heat treatment at 110
°C was undertaken, PMA pre-treatment did allow differentiation of non-infectious from infectious viruses. In this case, effective binding of PMA to bacteriophage T4 DNA could be taken to indicate capsid damage. Therefore, PMA pre-treatment may be appropriate for assessing effective disinfection treatments and for a more reliable understanding of the factors that contribute to viral inactivation through capsid damage monitoring. The PMA-PCR approach could be useful as a rapid and inexpensive analytical tool for screening and evaluation of the efficacy of disinfectants.</description><subject>Azides - metabolism</subject><subject>Bacteria</subject><subject>Bacteriologia</subject><subject>Bacteriology</subject><subject>Bacteriophage T4</subject><subject>Bacteriophage T4 - growth & development</subject><subject>Bacteriophage T4 - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Capsids</subject><subject>Ciències de la salut</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Intercalating Agents - metabolism</subject><subject>Medicina</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>PMA</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Propidium - analogs & derivatives</subject><subject>Propidium - metabolism</subject><subject>Real-time PCR</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling - methods</subject><subject>Techniques used in virology</subject><subject>Virology</subject><subject>Virology - methods</subject><subject>Virus</subject><subject>Virus infectivity</subject><subject>Viruses</subject><subject>Àrees temàtiques de la UPC</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>XX2</sourceid><recordid>eNqFUcFu1DAQtRCILoVfqHxBnLKM49iOb6At0EqVQKhI3CzHnoBXSbzYSaXy9TjaLT32YNkzes_z5j1CLhhsGTD5fr_d34UUR5y3NZQmyC0w9oxsWKt0BbptnpNNAcry5s0ZeZXzHgCE4vwlOatBaC0kbMjPy5BdCmOY7BziRGNPw9SjK8WSaWfdjCnEw2_7C-ltQ8vMtX1PDykegg_LSMc4Rfs3eKQJ7VDNYUT6bff9NXnR2yHjm9N9Tn58_nS7u6puvn653n28qVyj2FxxZL5rFQquXC2KYKytR-Wt4uA8NI3DRve9tlo4zVWnZK-Vs8z6uhOgOn5O2PFflxdnEjpMzs4m2vBYrKcGVRtWllaicN4dOWWLPwvm2YzFBRwGO2FZ27QFKyTX-kmkajSwVmpZkPKkI8WcE_bmUGy16d4wMGtkZm8eIjNrZAakKZEV4sVpxNKN6P_THjIqgLcngM3ODn2ykwv5EcehbUW9av1wxGGx-y5gMtkFnBz6UJyYjY_hKS3_AFQIuL0</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Fittipaldi, Mariana</creator><creator>Rodriguez, Nancy J. Pino</creator><creator>Codony, Francesc</creator><creator>Adrados, Bárbara</creator><creator>Peñuela, Gustavo A.</creator><creator>Morató, Jordi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>XX2</scope></search><sort><creationdate>20100901</creationdate><title>Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR</title><author>Fittipaldi, Mariana ; Rodriguez, Nancy J. Pino ; Codony, Francesc ; Adrados, Bárbara ; Peñuela, Gustavo A. ; Morató, Jordi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-3e1db87e537c25016e2ade7da730cd044ce49ff9a95c937b76f97ca1ad2b507b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Azides - metabolism</topic><topic>Bacteria</topic><topic>Bacteriologia</topic><topic>Bacteriology</topic><topic>Bacteriophage T4</topic><topic>Bacteriophage T4 - growth & development</topic><topic>Bacteriophage T4 - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Capsids</topic><topic>Ciències de la salut</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Intercalating Agents - metabolism</topic><topic>Medicina</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>PMA</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Propidium - analogs & derivatives</topic><topic>Propidium - metabolism</topic><topic>Real-time PCR</topic><topic>Sensitivity and Specificity</topic><topic>Specimen Handling - methods</topic><topic>Techniques used in virology</topic><topic>Virology</topic><topic>Virology - methods</topic><topic>Virus</topic><topic>Virus infectivity</topic><topic>Viruses</topic><topic>Àrees temàtiques de la UPC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fittipaldi, Mariana</creatorcontrib><creatorcontrib>Rodriguez, Nancy J. Pino</creatorcontrib><creatorcontrib>Codony, Francesc</creatorcontrib><creatorcontrib>Adrados, Bárbara</creatorcontrib><creatorcontrib>Peñuela, Gustavo A.</creatorcontrib><creatorcontrib>Morató, Jordi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Recercat</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fittipaldi, Mariana</au><au>Rodriguez, Nancy J. Pino</au><au>Codony, Francesc</au><au>Adrados, Bárbara</au><au>Peñuela, Gustavo A.</au><au>Morató, Jordi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2010-09-01</date><risdate>2010</risdate><volume>168</volume><issue>1</issue><spage>228</spage><epage>232</epage><pages>228-232</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>The advent of quantitative PCR has improved the detection of human viral pathogens in the environment. However, a serious limitation of this method may arise from the inability to discriminate between viruses that are infectious and viruses that have been inactivated and do not represent a human health hazard. To assess whether propidium monoazide (PMA) pre-treatment is a good approach to inhibiting DNA amplification from non-infectious viruses, bacteriophage T4 survival was measured using cell culture titration and real-time PCR with and without PMA pre-treatment. Heat (85
°C) and proteolysis methods were carried out. After these inactivation treatments, the results indicated that the PMA pre-treatment approach is not appropriate for differentiating infectious viruses. However, when a heat treatment at 110
°C was undertaken, PMA pre-treatment did allow differentiation of non-infectious from infectious viruses. In this case, effective binding of PMA to bacteriophage T4 DNA could be taken to indicate capsid damage. Therefore, PMA pre-treatment may be appropriate for assessing effective disinfection treatments and for a more reliable understanding of the factors that contribute to viral inactivation through capsid damage monitoring. The PMA-PCR approach could be useful as a rapid and inexpensive analytical tool for screening and evaluation of the efficacy of disinfectants.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>20599560</pmid><doi>10.1016/j.jviromet.2010.06.011</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 2010-09, Vol.168 (1), p.228-232 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_csuc_recercat_oai_recercat_cat_2072_195675 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Recercat |
| subjects | Azides - metabolism Bacteria Bacteriologia Bacteriology Bacteriophage T4 Bacteriophage T4 - growth & development Bacteriophage T4 - isolation & purification Biological and medical sciences Capsids Ciències de la salut Fundamental and applied biological sciences. Psychology Hot Temperature Humans Intercalating Agents - metabolism Medicina Microbial Viability Microbiology PMA Polymerase Chain Reaction - methods Propidium - analogs & derivatives Propidium - metabolism Real-time PCR Sensitivity and Specificity Specimen Handling - methods Techniques used in virology Virology Virology - methods Virus Virus infectivity Viruses Àrees temàtiques de la UPC |
| title | Discrimination of infectious bacteriophage T4 virus by propidium monoazide real-time PCR |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T04%3A34%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_csuc_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Discrimination%20of%20infectious%20bacteriophage%20T4%20virus%20by%20propidium%20monoazide%20real-time%20PCR&rft.jtitle=Journal%20of%20virological%20methods&rft.au=Fittipaldi,%20Mariana&rft.date=2010-09-01&rft.volume=168&rft.issue=1&rft.spage=228&rft.epage=232&rft.pages=228-232&rft.issn=0166-0934&rft.eissn=1879-0984&rft.coden=JVMEDH&rft_id=info:doi/10.1016/j.jviromet.2010.06.011&rft_dat=%3Cproquest_csuc_%3E807256399%3C/proquest_csuc_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=749018696&rft_id=info:pmid/20599560&rft_els_id=S0166093410002235&rfr_iscdi=true |