Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR
Background: While Helicobacter pylori exists in a bacillary form in both the natural habitat and the human host, detrimental environmental circumstances have been observed to lead to the conversion of H. pylori from the bacillary to the coccoid form. However, the viability or nonviability of coccoi...
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description | Background: While Helicobacter pylori exists in a bacillary form in both the natural habitat and the human host, detrimental environmental circumstances have been observed to lead to the conversion of H. pylori from the bacillary to the coccoid form. However, the viability or nonviability of coccoid forms remains to be established in H. pylori. The aim of this study was to determine whether the quantitative PCR combined with propidium monoazide could be an alternative and good technique to determine H. pylori viability in environmental samples and, to contribute to understanding of the role of the H. pylori forms.
Materials and Methods: Viability, morphological distribution, and the number of live H. pylori cells were determined using a propidium monoazide‐based quantitative PCR method, at various time points.
Results: Under adverse environmental conditions was observed the conversion of H. pylori from the bacillary to the coccoid form, and the decrease in amplification signal, in samples that were treated with propidium monoazide, over the time.
Conclusions: Incorporation of propidium monoazide indicates that there is an increase in H. pylori cells with the damaged membrane over the study, leading to the manifestation of cellular degeneration and death. Consequently, quantitative PCR combined with propidium monoazide contributes to our understanding of the role of H. pylori cells, under adverse environmental conditions. |
doi_str_mv | 10.1111/j.1523-5378.2010.00794.x |
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Materials and Methods: Viability, morphological distribution, and the number of live H. pylori cells were determined using a propidium monoazide‐based quantitative PCR method, at various time points.
Results: Under adverse environmental conditions was observed the conversion of H. pylori from the bacillary to the coccoid form, and the decrease in amplification signal, in samples that were treated with propidium monoazide, over the time.
Conclusions: Incorporation of propidium monoazide indicates that there is an increase in H. pylori cells with the damaged membrane over the study, leading to the manifestation of cellular degeneration and death. Consequently, quantitative PCR combined with propidium monoazide contributes to our understanding of the role of H. pylori cells, under adverse environmental conditions.</description><identifier>ISSN: 1083-4389</identifier><identifier>EISSN: 1523-5378</identifier><identifier>DOI: 10.1111/j.1523-5378.2010.00794.x</identifier><identifier>PMID: 21083754</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Azides - metabolism ; Bacteriologia ; Bacteriological Techniques - methods ; Cells ; cellular viability ; Ciències de la salut ; coccoid forms ; Cèl·lules ; Degeneration ; Environmental conditions ; Environmental Microbiology ; Enzyme Inhibitors - metabolism ; Habitat ; Helicobacter ; Helicobacter pylori ; Helicobacter pylori - cytology ; Helicobacter pylori - genetics ; Helicobacter pylori - physiology ; Humans ; Medicina ; Microbial Viability ; Microscopy ; Nanotechnology ; Nanotecnologia ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Propidium - analogs & derivatives ; Propidium - metabolism ; propidium monoazide ; qPCR ; Àrees temàtiques de la UPC</subject><ispartof>Helicobacter (Cambridge, Mass.), 2010-10, Vol.15 (5), p.473-476</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2010 Blackwell Publishing Ltd.</rights><rights>info:eu-repo/semantics/openAccess</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4794-4ae65d67095c9ae85d0d923e467efd8605ea9a96529c92366ed27e2efec6b29d3</citedby><cites>FETCH-LOGICAL-c4794-4ae65d67095c9ae85d0d923e467efd8605ea9a96529c92366ed27e2efec6b29d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1523-5378.2010.00794.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1523-5378.2010.00794.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,26974,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21083754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Agustí, Gemma</creatorcontrib><creatorcontrib>Codony, Francesc</creatorcontrib><creatorcontrib>Fittipaldi, Mariana</creatorcontrib><creatorcontrib>Adrados, Bárbara</creatorcontrib><creatorcontrib>Morató, Jordi</creatorcontrib><title>Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR</title><title>Helicobacter (Cambridge, Mass.)</title><addtitle>Helicobacter</addtitle><description>Background: While Helicobacter pylori exists in a bacillary form in both the natural habitat and the human host, detrimental environmental circumstances have been observed to lead to the conversion of H. pylori from the bacillary to the coccoid form. However, the viability or nonviability of coccoid forms remains to be established in H. pylori. The aim of this study was to determine whether the quantitative PCR combined with propidium monoazide could be an alternative and good technique to determine H. pylori viability in environmental samples and, to contribute to understanding of the role of the H. pylori forms.
Materials and Methods: Viability, morphological distribution, and the number of live H. pylori cells were determined using a propidium monoazide‐based quantitative PCR method, at various time points.
Results: Under adverse environmental conditions was observed the conversion of H. pylori from the bacillary to the coccoid form, and the decrease in amplification signal, in samples that were treated with propidium monoazide, over the time.
Conclusions: Incorporation of propidium monoazide indicates that there is an increase in H. pylori cells with the damaged membrane over the study, leading to the manifestation of cellular degeneration and death. Consequently, quantitative PCR combined with propidium monoazide contributes to our understanding of the role of H. pylori cells, under adverse environmental conditions.</description><subject>Azides - metabolism</subject><subject>Bacteriologia</subject><subject>Bacteriological Techniques - methods</subject><subject>Cells</subject><subject>cellular viability</subject><subject>Ciències de la salut</subject><subject>coccoid forms</subject><subject>Cèl·lules</subject><subject>Degeneration</subject><subject>Environmental conditions</subject><subject>Environmental Microbiology</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Habitat</subject><subject>Helicobacter</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - cytology</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - physiology</subject><subject>Humans</subject><subject>Medicina</subject><subject>Microbial Viability</subject><subject>Microscopy</subject><subject>Nanotechnology</subject><subject>Nanotecnologia</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Propidium - analogs & derivatives</subject><subject>Propidium - metabolism</subject><subject>propidium monoazide</subject><subject>qPCR</subject><subject>Àrees temàtiques de la UPC</subject><issn>1083-4389</issn><issn>1523-5378</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>XX2</sourceid><recordid>eNqNkd9v0zAQxyMEYmPwLyC_8ZTiOP4RS7ygMlakMjbENsHLyXUuyCWJi51Ay1-Ps5byCJYsn---n7uTvllGCjor0nm5nhWClbkoVTVjNGUpVZrPtg-y02PhYYppVea8rPRJ9iTGNaVUlFw_zk7YVFGCn2afb51ZudYNO_IGBwyd683gfE98QxbYOutXxqY82exaHxy5ia7_Sq6C37jajR1573tvfrkayfVo-sENif6B5Gr-8Wn2qDFtxGeH9yy7eXv-ab7Ilx8u3s1fL3PL0845NyhFLRXVwmqDlahprVmJXCps6kpSgUYbLQXTNuWlxJopZNiglSum6_IsK_Z9bRwtBLQYrBnAG_f3M11GFYNCM8FUYl7smU3w30eMA3QuWmxb06MfI6SxZcW4Yv9W0opLru97Voc9go8xYAOb4DoTdlBQmFyDNUzmwGQOTK7BvWuwTejzw5Bx1WF9BP_YlASv9oKfrsXdfzeGxfkyBQnP97iLA26PuAnfQKo0AO4uL6C4W9zyL9cCLsvfxqu1Ag</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Agustí, Gemma</creator><creator>Codony, Francesc</creator><creator>Fittipaldi, Mariana</creator><creator>Adrados, Bárbara</creator><creator>Morató, Jordi</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>XX2</scope></search><sort><creationdate>201010</creationdate><title>Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR</title><author>Agustí, Gemma ; Codony, Francesc ; Fittipaldi, Mariana ; Adrados, Bárbara ; Morató, Jordi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4794-4ae65d67095c9ae85d0d923e467efd8605ea9a96529c92366ed27e2efec6b29d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Azides - metabolism</topic><topic>Bacteriologia</topic><topic>Bacteriological Techniques - methods</topic><topic>Cells</topic><topic>cellular viability</topic><topic>Ciències de la salut</topic><topic>coccoid forms</topic><topic>Cèl·lules</topic><topic>Degeneration</topic><topic>Environmental conditions</topic><topic>Environmental Microbiology</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Habitat</topic><topic>Helicobacter</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - cytology</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - physiology</topic><topic>Humans</topic><topic>Medicina</topic><topic>Microbial Viability</topic><topic>Microscopy</topic><topic>Nanotechnology</topic><topic>Nanotecnologia</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Propidium - analogs & derivatives</topic><topic>Propidium - metabolism</topic><topic>propidium monoazide</topic><topic>qPCR</topic><topic>Àrees temàtiques de la UPC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Agustí, Gemma</creatorcontrib><creatorcontrib>Codony, Francesc</creatorcontrib><creatorcontrib>Fittipaldi, Mariana</creatorcontrib><creatorcontrib>Adrados, Bárbara</creatorcontrib><creatorcontrib>Morató, Jordi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Recercat</collection><jtitle>Helicobacter (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agustí, Gemma</au><au>Codony, Francesc</au><au>Fittipaldi, Mariana</au><au>Adrados, Bárbara</au><au>Morató, Jordi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR</atitle><jtitle>Helicobacter (Cambridge, Mass.)</jtitle><addtitle>Helicobacter</addtitle><date>2010-10</date><risdate>2010</risdate><volume>15</volume><issue>5</issue><spage>473</spage><epage>476</epage><pages>473-476</pages><issn>1083-4389</issn><eissn>1523-5378</eissn><abstract>Background: While Helicobacter pylori exists in a bacillary form in both the natural habitat and the human host, detrimental environmental circumstances have been observed to lead to the conversion of H. pylori from the bacillary to the coccoid form. However, the viability or nonviability of coccoid forms remains to be established in H. pylori. The aim of this study was to determine whether the quantitative PCR combined with propidium monoazide could be an alternative and good technique to determine H. pylori viability in environmental samples and, to contribute to understanding of the role of the H. pylori forms.
Materials and Methods: Viability, morphological distribution, and the number of live H. pylori cells were determined using a propidium monoazide‐based quantitative PCR method, at various time points.
Results: Under adverse environmental conditions was observed the conversion of H. pylori from the bacillary to the coccoid form, and the decrease in amplification signal, in samples that were treated with propidium monoazide, over the time.
Conclusions: Incorporation of propidium monoazide indicates that there is an increase in H. pylori cells with the damaged membrane over the study, leading to the manifestation of cellular degeneration and death. Consequently, quantitative PCR combined with propidium monoazide contributes to our understanding of the role of H. pylori cells, under adverse environmental conditions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21083754</pmid><doi>10.1111/j.1523-5378.2010.00794.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Azides - metabolism Bacteriologia Bacteriological Techniques - methods Cells cellular viability Ciències de la salut coccoid forms Cèl·lules Degeneration Environmental conditions Environmental Microbiology Enzyme Inhibitors - metabolism Habitat Helicobacter Helicobacter pylori Helicobacter pylori - cytology Helicobacter pylori - genetics Helicobacter pylori - physiology Humans Medicina Microbial Viability Microscopy Nanotechnology Nanotecnologia Polymerase chain reaction Polymerase Chain Reaction - methods Propidium - analogs & derivatives Propidium - metabolism propidium monoazide qPCR Àrees temàtiques de la UPC |
title | Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR |
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