Influence of Some Antibiotics and Essential Oils Used Alone or in Combination on the Vitality of Presumptive Probiotic Lactic acid Bacteria

Aims: The aim of this study was to assess the in vitro antibacterial activity of selected antibiotics and essential oils alone or in combination, on selected presumptive probiotic lactic acid bacteria. Study Design: Experimental studies. Place and Duration of Study: Department of Microbiology of the University of Yaounde I between August 2017 and December 2017 (5 months). Methodology: The chemical composition of five essential oils was determined by gas chromatography coupled with Solid-phase micro extraction. Then the sensitivity of four lactic acid bacteria to the essential oils and four antibiotics was assessed by the well diffusion and macrodilution method. Subsequently, two essential oils active on these bacteria and broad spectrum antibiotics were combined according to the central composite design plan. Results: In general, the chemical composition of essential oils is very diverse, with the example of carvacrol found only in Origanum compactum at 53.24% and thymol in Thymus vulgaris at 56.19% and in Origanum compactum at 15.28%. The antibacterial activity shows that the majority of antibiotics used are active on the bacteria in the study compared to the essential oils where two were active (Origanum compactum and Cymbopogon winterianus). The evaluation of the combinations of essential oils and antibiotics in terms of kinetics has given us three cases: the first case is the one with no acidity or no growth at all; the second is the one where growth is normal; the third where growth is delayed with a more pronounced latency phase. Conclusion: This study suggest that the effect of essential oils and medicinal plant used alone or in combination to antibiotics on the gut microbiota have to be evaluated for validation as well as their toxicity activities before using them for human therapy.