Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA
Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River...
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| Veröffentlicht in: | Journal of wildlife diseases 2020-07, Vol.56 (3), p.702-706 |
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| container_title | Journal of wildlife diseases |
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| creator | Barnes, Matthew A. Brown, Audrey D. Daum, Mikaela N. de la Garza, Karla A. Driskill, Jackson Garrett, Kylie Goldstein, Madeleine S. Luk, Alaia Maguire, Joel I. Moke, Robert Ostermaier, Emily M. Sanders, Yorick M. Sandhu, Theodore Stith, Aryiah Suresh, Varshini V. |
| description | Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region. |
| doi_str_mv | 10.7589/2019-08-212 |
| format | Article |
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The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.</description><identifier>ISSN: 0090-3558</identifier><identifier>EISSN: 1943-3700</identifier><identifier>DOI: 10.7589/2019-08-212</identifier><identifier>PMID: 32243244</identifier><language>eng</language><publisher>United States: Wildlife Disease Association</publisher><subject>Amphibian disease ; Amphibians ; Animals ; Batrachochytrium - isolation & purification ; chytrid fungus ; DNA Virus Infections - epidemiology ; DNA Virus Infections - veterinary ; DNA Virus Infections - virology ; eDNA ; Environmental DNA - genetics ; Environmental DNA - isolation & purification ; Mycoses - epidemiology ; Mycoses - microbiology ; Mycoses - veterinary ; Ranavirus ; Ranavirus - isolation & purification ; Rivers ; SHORT COMMUNICATIONS ; Texas ; west Texas</subject><ispartof>Journal of wildlife diseases, 2020-07, Vol.56 (3), p.702-706</ispartof><rights>Wildlife Disease Association 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b323t-e9c36865fb69912891a4f25ec124a352be6d802a4f8ef0a6aa2fbbfa4cb35c33</citedby><cites>FETCH-LOGICAL-b323t-e9c36865fb69912891a4f25ec124a352be6d802a4f8ef0a6aa2fbbfa4cb35c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32243244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barnes, Matthew A.</creatorcontrib><creatorcontrib>Brown, Audrey D.</creatorcontrib><creatorcontrib>Daum, Mikaela N.</creatorcontrib><creatorcontrib>de la Garza, Karla A.</creatorcontrib><creatorcontrib>Driskill, Jackson</creatorcontrib><creatorcontrib>Garrett, Kylie</creatorcontrib><creatorcontrib>Goldstein, Madeleine S.</creatorcontrib><creatorcontrib>Luk, Alaia</creatorcontrib><creatorcontrib>Maguire, Joel I.</creatorcontrib><creatorcontrib>Moke, Robert</creatorcontrib><creatorcontrib>Ostermaier, Emily M.</creatorcontrib><creatorcontrib>Sanders, Yorick M.</creatorcontrib><creatorcontrib>Sandhu, Theodore</creatorcontrib><creatorcontrib>Stith, Aryiah</creatorcontrib><creatorcontrib>Suresh, Varshini V.</creatorcontrib><title>Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA</title><title>Journal of wildlife diseases</title><addtitle>J Wildl Dis</addtitle><description>Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. The amphibian pathogens Batrachochytrium dendrobatidis (Bd) and Ranavirus have been relatively understudied in Texas, US, so we applied eDNA assays for the surveillance of these pathogens in the upper Brazos River basin near the Texas panhandle. We collected water samples from five urban playa lakes and one reservoir in and around Lubbock, Texas. Quantitative PCR detected both Bd and Ranavirus at one playa lake, representing novel detection of both pathogens in the region. Based on these results, we recommend increased monitoring for the pathogens and symptoms of amphibian disease throughout the region.</description><subject>Amphibian disease</subject><subject>Amphibians</subject><subject>Animals</subject><subject>Batrachochytrium - isolation & purification</subject><subject>chytrid fungus</subject><subject>DNA Virus Infections - epidemiology</subject><subject>DNA Virus Infections - veterinary</subject><subject>DNA Virus Infections - virology</subject><subject>eDNA</subject><subject>Environmental DNA - genetics</subject><subject>Environmental DNA - isolation & purification</subject><subject>Mycoses - epidemiology</subject><subject>Mycoses - microbiology</subject><subject>Mycoses - veterinary</subject><subject>Ranavirus</subject><subject>Ranavirus - isolation & purification</subject><subject>Rivers</subject><subject>SHORT COMMUNICATIONS</subject><subject>Texas</subject><subject>west Texas</subject><issn>0090-3558</issn><issn>1943-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAURS0EoqUwsSOPVBBw7CR1xtAPQEKAoIgxek6cxqixq9hB9E_wm3EpMLK8q3d1dIeD0HFILkYxTy8pCdOA8ICGdAf1wzRiARsRsov6hKQkYHHMe-jA2jdCaOyffdRjlEaMRlEffU6kk4VTRmNTYVdLnDWrWgkFGj-Cq81CaovH9dq1qsSzTi86i0-vwLVQ1Kb47rsGl1KXrRHgVKnsEIMu8RNoeFetx5XGr9I6PJcfYM_xy3Pmj1V6gafaE0Y3UjtY4sl9doj2KlhaefSTAzSfTefjm-Du4fp2nN0FglHmApkWLOFJXIkkTUPK0xCiisayCGkELKZCJiUn1JdcVgQSAFoJUUFUCBYXjA3Q2Xa2aI21razyVasaaNd5SPKN1HwjNSc891I9fbKlV51oZPnH_lr0wHALCGWMlv-OfQGdeYA_</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Barnes, Matthew A.</creator><creator>Brown, Audrey D.</creator><creator>Daum, Mikaela N.</creator><creator>de la Garza, Karla A.</creator><creator>Driskill, Jackson</creator><creator>Garrett, Kylie</creator><creator>Goldstein, Madeleine S.</creator><creator>Luk, Alaia</creator><creator>Maguire, Joel I.</creator><creator>Moke, Robert</creator><creator>Ostermaier, Emily M.</creator><creator>Sanders, Yorick M.</creator><creator>Sandhu, Theodore</creator><creator>Stith, Aryiah</creator><creator>Suresh, Varshini V.</creator><general>Wildlife Disease Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200701</creationdate><title>Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA</title><author>Barnes, Matthew A. ; Brown, Audrey D. ; Daum, Mikaela N. ; de la Garza, Karla A. ; Driskill, Jackson ; Garrett, Kylie ; Goldstein, Madeleine S. ; Luk, Alaia ; Maguire, Joel I. ; Moke, Robert ; Ostermaier, Emily M. ; Sanders, Yorick M. ; Sandhu, Theodore ; Stith, Aryiah ; Suresh, Varshini V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b323t-e9c36865fb69912891a4f25ec124a352be6d802a4f8ef0a6aa2fbbfa4cb35c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amphibian disease</topic><topic>Amphibians</topic><topic>Animals</topic><topic>Batrachochytrium - isolation & purification</topic><topic>chytrid fungus</topic><topic>DNA Virus Infections - epidemiology</topic><topic>DNA Virus Infections - veterinary</topic><topic>DNA Virus Infections - virology</topic><topic>eDNA</topic><topic>Environmental DNA - genetics</topic><topic>Environmental DNA - isolation & purification</topic><topic>Mycoses - epidemiology</topic><topic>Mycoses - microbiology</topic><topic>Mycoses - veterinary</topic><topic>Ranavirus</topic><topic>Ranavirus - isolation & purification</topic><topic>Rivers</topic><topic>SHORT COMMUNICATIONS</topic><topic>Texas</topic><topic>west Texas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barnes, Matthew A.</creatorcontrib><creatorcontrib>Brown, Audrey D.</creatorcontrib><creatorcontrib>Daum, Mikaela N.</creatorcontrib><creatorcontrib>de la Garza, Karla A.</creatorcontrib><creatorcontrib>Driskill, Jackson</creatorcontrib><creatorcontrib>Garrett, Kylie</creatorcontrib><creatorcontrib>Goldstein, Madeleine S.</creatorcontrib><creatorcontrib>Luk, Alaia</creatorcontrib><creatorcontrib>Maguire, Joel I.</creatorcontrib><creatorcontrib>Moke, Robert</creatorcontrib><creatorcontrib>Ostermaier, Emily M.</creatorcontrib><creatorcontrib>Sanders, Yorick M.</creatorcontrib><creatorcontrib>Sandhu, Theodore</creatorcontrib><creatorcontrib>Stith, Aryiah</creatorcontrib><creatorcontrib>Suresh, Varshini V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of wildlife diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barnes, Matthew A.</au><au>Brown, Audrey D.</au><au>Daum, Mikaela N.</au><au>de la Garza, Karla A.</au><au>Driskill, Jackson</au><au>Garrett, Kylie</au><au>Goldstein, Madeleine S.</au><au>Luk, Alaia</au><au>Maguire, Joel I.</au><au>Moke, Robert</au><au>Ostermaier, Emily M.</au><au>Sanders, Yorick M.</au><au>Sandhu, Theodore</au><au>Stith, Aryiah</au><au>Suresh, Varshini V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA</atitle><jtitle>Journal of wildlife diseases</jtitle><addtitle>J Wildl Dis</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>56</volume><issue>3</issue><spage>702</spage><epage>706</epage><pages>702-706</pages><issn>0090-3558</issn><eissn>1943-3700</eissn><abstract>Environmental DNA (eDNA) methods provide novel options for the detection of pathogens. 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| ispartof | Journal of wildlife diseases, 2020-07, Vol.56 (3), p.702-706 |
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| language | eng |
| recordid | cdi_crossref_primary_10_7589_2019_08_212 |
| source | MEDLINE; Allen Press Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Amphibian disease Amphibians Animals Batrachochytrium - isolation & purification chytrid fungus DNA Virus Infections - epidemiology DNA Virus Infections - veterinary DNA Virus Infections - virology eDNA Environmental DNA - genetics Environmental DNA - isolation & purification Mycoses - epidemiology Mycoses - microbiology Mycoses - veterinary Ranavirus Ranavirus - isolation & purification Rivers SHORT COMMUNICATIONS Texas west Texas |
| title | Detection of the Amphibian Pathogens Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in West Texas, USA, Using Environmental DNA |
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