Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus
Nitrosoguanidine-induced melanin-negative mutants of Streptomyces griseus were classified according to their ability to produce streptomycin and A-factor, and to form aerial mycelium. A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or...
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| Veröffentlicht in: | Journal of antibiotics 2001/10/25, Vol.54(10), pp.789-796 |
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| creator | ENDO, KOHKI KAMO, KEIKO HOSONO, KUNIAKI BEPPU, TERUHIKO UEDA, KENJI |
| description | Nitrosoguanidine-induced melanin-negative mutants of Streptomyces griseus were classified according to their ability to produce streptomycin and A-factor, and to form aerial mycelium. A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or both, suggesting close regulatory correlation between melanogenesis and morphological and physiological differentiation. The tyrosinase-encoding mel operon of S. griseus was cloned and examined for its role in melanogenesis of this organism. As in other Streptomyces homologues, the operon consisted of two open reading frames, melCl encoding the putative cofactor and melC2 encoding the tyrosinase. Regardless of the distinct sequence similarity, introduction of the operon on plasmids failed to confer melanin production in the melanin-negative mutants, and the disruption of melC2 barely affected the melanin productivity, which indicated the presence of another enzyme involved in the melanogenesis in S. griseus. On the other hand, mel on a high-copy-number plasmid caused precocious aerial mycelium formation in Streptomyces lividans TK21 suggesting a stimulatory role of tyrosinase in morphological development. |
| doi_str_mv | 10.7164/antibiotics.54.789 |
| format | Article |
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A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or both, suggesting close regulatory correlation between melanogenesis and morphological and physiological differentiation. The tyrosinase-encoding mel operon of S. griseus was cloned and examined for its role in melanogenesis of this organism. As in other Streptomyces homologues, the operon consisted of two open reading frames, melCl encoding the putative cofactor and melC2 encoding the tyrosinase. Regardless of the distinct sequence similarity, introduction of the operon on plasmids failed to confer melanin production in the melanin-negative mutants, and the disruption of melC2 barely affected the melanin productivity, which indicated the presence of another enzyme involved in the melanogenesis in S. griseus. On the other hand, mel on a high-copy-number plasmid caused precocious aerial mycelium formation in Streptomyces lividans TK21 suggesting a stimulatory role of tyrosinase in morphological development.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.7164/antibiotics.54.789</identifier><identifier>PMID: 11776433</identifier><identifier>CODEN: JANTAJ</identifier><language>eng</language><publisher>Tokyo: JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - biosynthesis ; Amino Acid Sequence ; Anti-Bacterial Agents - biosynthesis ; Biological and medical sciences ; Cell Differentiation - physiology ; Cloning, Molecular ; DNA, Fungal - biosynthesis ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Melanins - biosynthesis ; Melanins - genetics ; Microbiology ; Molecular Sequence Data ; Monophenol Monooxygenase - genetics ; Mutagenesis - drug effects ; Mutagens - pharmacology ; Mutation - drug effects ; Mycology ; Nitrosoguanidines - pharmacology ; Open Reading Frames - genetics ; Phenotype ; Plasmids - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Streptomyces griseus - genetics ; Streptomyces griseus - growth & development ; Streptomyces griseus - metabolism ; Streptomycin - biosynthesis</subject><ispartof>The Journal of Antibiotics, 2001/10/25, Vol.54(10), pp.789-796</ispartof><rights>Japan Antibiotics Research Association</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c639t-33b35451ccb81363b523d357e381490df0e0757d65bf9da3a97e5c77e956eab53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14111528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11776433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ENDO, KOHKI</creatorcontrib><creatorcontrib>KAMO, KEIKO</creatorcontrib><creatorcontrib>HOSONO, KUNIAKI</creatorcontrib><creatorcontrib>BEPPU, TERUHIKO</creatorcontrib><creatorcontrib>UEDA, KENJI</creatorcontrib><title>Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus</title><title>Journal of antibiotics</title><addtitle>J. Antibiot.</addtitle><description>Nitrosoguanidine-induced melanin-negative mutants of Streptomyces griseus were classified according to their ability to produce streptomycin and A-factor, and to form aerial mycelium. A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or both, suggesting close regulatory correlation between melanogenesis and morphological and physiological differentiation. The tyrosinase-encoding mel operon of S. griseus was cloned and examined for its role in melanogenesis of this organism. As in other Streptomyces homologues, the operon consisted of two open reading frames, melCl encoding the putative cofactor and melC2 encoding the tyrosinase. Regardless of the distinct sequence similarity, introduction of the operon on plasmids failed to confer melanin production in the melanin-negative mutants, and the disruption of melC2 barely affected the melanin productivity, which indicated the presence of another enzyme involved in the melanogenesis in S. griseus. On the other hand, mel on a high-copy-number plasmid caused precocious aerial mycelium formation in Streptomyces lividans TK21 suggesting a stimulatory role of tyrosinase in morphological development.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - biosynthesis</subject><subject>Amino Acid Sequence</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - physiology</subject><subject>Cloning, Molecular</subject><subject>DNA, Fungal - biosynthesis</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Melanins - biosynthesis</subject><subject>Melanins - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>Mutagenesis - drug effects</subject><subject>Mutagens - pharmacology</subject><subject>Mutation - drug effects</subject><subject>Mycology</subject><subject>Nitrosoguanidines - pharmacology</subject><subject>Open Reading Frames - genetics</subject><subject>Phenotype</subject><subject>Plasmids - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Streptomyces griseus - genetics</subject><subject>Streptomyces griseus - growth & development</subject><subject>Streptomyces griseus - metabolism</subject><subject>Streptomycin - biosynthesis</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU9LAzEQxYMotv75Ah4kF49bk81mkz1K1Sq0eFDPy2x2tkbabElSoX56U1pqLxMGfm_e44WQG85GipfFPbhoG9tHa8JIFiOlqxMy5FrzjBdldUqGjOU80zpnA3IRwjdjQgmlz8mAc6XKQogh-Rp_gQcT0dtfiLZ3tO_obB3T7UAfsUMT7Q9S6-gMF-D6OToMNlBwLQU6SRvtek8_Nr4P1kHArf49elzFfrkxGOjc24DrcEXOOlgEvN6_l-Tz-elj_JJN3yav44dpZkpRxUyIRshCcmMazUUpGpmLVkiFQvOiYm3HkCmp2lI2XdWCgEqhNEphJUuERopLku_umpQoeOzqlbdL8Juas3pbW31UWy2LOtWWRLc70WrdLLH9l-x7SsDdHoBgYNF5cMaGf67gnMtcJ262475DhDkeAPDJboHH3rwq9dY_xdrNFOTAmfQrNTrxB8GjlmQ</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>ENDO, KOHKI</creator><creator>KAMO, KEIKO</creator><creator>HOSONO, KUNIAKI</creator><creator>BEPPU, TERUHIKO</creator><creator>UEDA, KENJI</creator><general>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</general><general>Japan Antibiotics Research Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2001</creationdate><title>Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus</title><author>ENDO, KOHKI ; KAMO, KEIKO ; HOSONO, KUNIAKI ; BEPPU, TERUHIKO ; UEDA, KENJI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c639t-33b35451ccb81363b523d357e381490df0e0757d65bf9da3a97e5c77e956eab53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - biosynthesis</topic><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - physiology</topic><topic>Cloning, Molecular</topic><topic>DNA, Fungal - biosynthesis</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Melanins - biosynthesis</topic><topic>Melanins - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Monophenol Monooxygenase - genetics</topic><topic>Mutagenesis - drug effects</topic><topic>Mutagens - pharmacology</topic><topic>Mutation - drug effects</topic><topic>Mycology</topic><topic>Nitrosoguanidines - pharmacology</topic><topic>Open Reading Frames - genetics</topic><topic>Phenotype</topic><topic>Plasmids - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Streptomyces griseus - genetics</topic><topic>Streptomyces griseus - growth & development</topic><topic>Streptomyces griseus - metabolism</topic><topic>Streptomycin - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ENDO, KOHKI</creatorcontrib><creatorcontrib>KAMO, KEIKO</creatorcontrib><creatorcontrib>HOSONO, KUNIAKI</creatorcontrib><creatorcontrib>BEPPU, TERUHIKO</creatorcontrib><creatorcontrib>UEDA, KENJI</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of antibiotics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ENDO, KOHKI</au><au>KAMO, KEIKO</au><au>HOSONO, KUNIAKI</au><au>BEPPU, TERUHIKO</au><au>UEDA, KENJI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus</atitle><jtitle>Journal of antibiotics</jtitle><addtitle>J. Antibiot.</addtitle><date>2001</date><risdate>2001</risdate><volume>54</volume><issue>10</issue><spage>789</spage><epage>796</epage><pages>789-796</pages><issn>0021-8820</issn><eissn>1881-1469</eissn><coden>JANTAJ</coden><abstract>Nitrosoguanidine-induced melanin-negative mutants of Streptomyces griseus were classified according to their ability to produce streptomycin and A-factor, and to form aerial mycelium. A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or both, suggesting close regulatory correlation between melanogenesis and morphological and physiological differentiation. The tyrosinase-encoding mel operon of S. griseus was cloned and examined for its role in melanogenesis of this organism. As in other Streptomyces homologues, the operon consisted of two open reading frames, melCl encoding the putative cofactor and melC2 encoding the tyrosinase. Regardless of the distinct sequence similarity, introduction of the operon on plasmids failed to confer melanin production in the melanin-negative mutants, and the disruption of melC2 barely affected the melanin productivity, which indicated the presence of another enzyme involved in the melanogenesis in S. griseus. On the other hand, mel on a high-copy-number plasmid caused precocious aerial mycelium formation in Streptomyces lividans TK21 suggesting a stimulatory role of tyrosinase in morphological development.</abstract><cop>Tokyo</cop><pub>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</pub><pmid>11776433</pmid><doi>10.7164/antibiotics.54.789</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of Antibiotics, 2001/10/25, Vol.54(10), pp.789-796 |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - biosynthesis Amino Acid Sequence Anti-Bacterial Agents - biosynthesis Biological and medical sciences Cell Differentiation - physiology Cloning, Molecular DNA, Fungal - biosynthesis DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Melanins - biosynthesis Melanins - genetics Microbiology Molecular Sequence Data Monophenol Monooxygenase - genetics Mutagenesis - drug effects Mutagens - pharmacology Mutation - drug effects Mycology Nitrosoguanidines - pharmacology Open Reading Frames - genetics Phenotype Plasmids - genetics Reverse Transcriptase Polymerase Chain Reaction Streptomyces griseus - genetics Streptomyces griseus - growth & development Streptomyces griseus - metabolism Streptomycin - biosynthesis |
| title | Characterization of Mutants Defective in Melanogenesis and a Gene for Tyrosinase of Streptomyces griseus |
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