Study on Analysis of N-Nitroso Compounds in the Environment (II): Determination of Nitrite in Environmental Samples by HPLC using Diazotization Reaction

A new methods have been developped for the determination of nitrite in the environmental water samples by HPLC with post-column and the flow injection analysis (FIA) using diazotization reaction. The optimum temperature and reaction time were investigated on the diazotization reaction of nitrite. We could detect 0.001ppm of nitrite by HPLC and FIA using diazotization reaction and spectrophotometric detection where 540 nm of wave length was used. But it was observed in this method that some interferences existed. Finally, we determined successfully nitrite in samples conteining high concentration of chloride such as tap and sea water by post-column HPLC using derivarization with diazonium salt.