Application of enzyme immunoassay kits for the determination of pig plasma progesterone levels
Enzyme immunoassay kits, Ovucheck Plasma/Serum Progesterone EIA Kit (EIA, Cambridge Veterinary Science Ltd) and Ovucheck Cow Side W (Cow Side W, Cambridge Veterinary Science Ltd), were applied to determination of pigs plasma progesterone (P) levels. We tried to study the modification of EIA assay sy...
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| Veröffentlicht in: | Japanese Journal of Swine Science (Japan) 1994/09/16, Vol.31(3), pp.109-115 |
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| container_title | Japanese Journal of Swine Science (Japan) |
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| creator | Kamei, M. (Kanagawa-ken. Livestock Experiment Station, Ebina (Japan)) Iwamura, S Yoshioka, K Kamomae, H |
| description | Enzyme immunoassay kits, Ovucheck Plasma/Serum Progesterone EIA Kit (EIA, Cambridge Veterinary Science Ltd) and Ovucheck Cow Side W (Cow Side W, Cambridge Veterinary Science Ltd), were applied to determination of pigs plasma progesterone (P) levels. We tried to study the modification of EIA assay system for pig P measurement; ie., examined for best dilution methodes with castrated serum/plasma, boar/sow serum and normal saline as dilution solution with 2, 5 and 10 times dilution. All solutions have no effect on the plasma P levels, but castrated serum/plasma and boar serum showed much more suitable for plasma dilution in 2 and 5 times dilution. Porcine plasma P levels assyed by EIA with and without dilution showed significant positive correlation with those by RIA. Cow Side W is used as a qualitative assay system, but it seems to be usable for a semiquantitative assay when using known value solution as standard. Plasma dilution with pigs serum have no effect on Cow Side W assay system too. We demonstrated that modification in assay methods of commercial EIA kits developed for cow were applicable for the determination of pig plasma P levels. Then these system would supply a suitable tool for the diagnosis of pregnancy and ovarian function in pigs. |
| doi_str_mv | 10.5938/youton.31.109 |
| format | Article |
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(Kanagawa-ken. Livestock Experiment Station, Ebina (Japan)) ; Iwamura, S ; Yoshioka, K ; Kamomae, H</creator><creatorcontrib>Kamei, M. (Kanagawa-ken. Livestock Experiment Station, Ebina (Japan)) ; Iwamura, S ; Yoshioka, K ; Kamomae, H</creatorcontrib><description>Enzyme immunoassay kits, Ovucheck Plasma/Serum Progesterone EIA Kit (EIA, Cambridge Veterinary Science Ltd) and Ovucheck Cow Side W (Cow Side W, Cambridge Veterinary Science Ltd), were applied to determination of pigs plasma progesterone (P) levels. We tried to study the modification of EIA assay system for pig P measurement; ie., examined for best dilution methodes with castrated serum/plasma, boar/sow serum and normal saline as dilution solution with 2, 5 and 10 times dilution. All solutions have no effect on the plasma P levels, but castrated serum/plasma and boar serum showed much more suitable for plasma dilution in 2 and 5 times dilution. Porcine plasma P levels assyed by EIA with and without dilution showed significant positive correlation with those by RIA. Cow Side W is used as a qualitative assay system, but it seems to be usable for a semiquantitative assay when using known value solution as standard. Plasma dilution with pigs serum have no effect on Cow Side W assay system too. We demonstrated that modification in assay methods of commercial EIA kits developed for cow were applicable for the determination of pig plasma P levels. Then these system would supply a suitable tool for the diagnosis of pregnancy and ovarian function in pigs.</description><identifier>ISSN: 0913-882X</identifier><identifier>EISSN: 1881-655X</identifier><identifier>DOI: 10.5938/youton.31.109</identifier><language>jpn</language><publisher>The Japanese Society of Swine Science</publisher><subject>ANALYTICAL METHODS ; BLOOD PLASMA ; CERDO ; enzyme immunoassay ; EQUIPO ; IMMUNOENZYME TECHNIQUES ; MATERIEL ; Ovucheck EIA Kit ; PLASMA SANGUIN ; PLASMA SANGUINEO ; PORCIN ; progesteron ; PROGESTERONA ; PROGESTERONE ; radio immunoassay ; SWINE ; TECHNIQUE ANALYTIQUE ; TECHNIQUE IMMUNOENZYMATIQUE ; TECNICAS ANALITICAS ; TECNICAS INMUNOENZIMATICAS</subject><ispartof>Nihon Yoton Gakkaishi, 1994/09/16, Vol.31(3), pp.109-115</ispartof><rights>The Japanese Society of Swine Science</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2609-1796a0054e50b695547c02bf63f7debb4937168cf127e40e342e43c7af70091d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Kamei, M. (Kanagawa-ken. Livestock Experiment Station, Ebina (Japan))</creatorcontrib><creatorcontrib>Iwamura, S</creatorcontrib><creatorcontrib>Yoshioka, K</creatorcontrib><creatorcontrib>Kamomae, H</creatorcontrib><title>Application of enzyme immunoassay kits for the determination of pig plasma progesterone levels</title><title>Japanese Journal of Swine Science (Japan)</title><addtitle>Nihon Yoton Gakkaishi</addtitle><description>Enzyme immunoassay kits, Ovucheck Plasma/Serum Progesterone EIA Kit (EIA, Cambridge Veterinary Science Ltd) and Ovucheck Cow Side W (Cow Side W, Cambridge Veterinary Science Ltd), were applied to determination of pigs plasma progesterone (P) levels. We tried to study the modification of EIA assay system for pig P measurement; ie., examined for best dilution methodes with castrated serum/plasma, boar/sow serum and normal saline as dilution solution with 2, 5 and 10 times dilution. All solutions have no effect on the plasma P levels, but castrated serum/plasma and boar serum showed much more suitable for plasma dilution in 2 and 5 times dilution. Porcine plasma P levels assyed by EIA with and without dilution showed significant positive correlation with those by RIA. Cow Side W is used as a qualitative assay system, but it seems to be usable for a semiquantitative assay when using known value solution as standard. Plasma dilution with pigs serum have no effect on Cow Side W assay system too. We demonstrated that modification in assay methods of commercial EIA kits developed for cow were applicable for the determination of pig plasma P levels. Then these system would supply a suitable tool for the diagnosis of pregnancy and ovarian function in pigs.</description><subject>ANALYTICAL METHODS</subject><subject>BLOOD PLASMA</subject><subject>CERDO</subject><subject>enzyme immunoassay</subject><subject>EQUIPO</subject><subject>IMMUNOENZYME TECHNIQUES</subject><subject>MATERIEL</subject><subject>Ovucheck EIA Kit</subject><subject>PLASMA SANGUIN</subject><subject>PLASMA SANGUINEO</subject><subject>PORCIN</subject><subject>progesteron</subject><subject>PROGESTERONA</subject><subject>PROGESTERONE</subject><subject>radio immunoassay</subject><subject>SWINE</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECHNIQUE IMMUNOENZYMATIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>TECNICAS INMUNOENZIMATICAS</subject><issn>0913-882X</issn><issn>1881-655X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo9kEtrwzAQhEVpoaHNsZee9AecSpasxzGEpg8C7aGFnGpkZ-WotS0jOQX319fBIZdd2P1mYAahO0oWmWbqYfCH3rcLRheU6As0o0rRRGTZ9hLNiKYsUSrdXqN5jK4gJFVECMJn6GvZdbUrTe98i73F0P4NDWDXNIfWmxjNgH9cH7H1Afd7wDvoITSuPQs6V-GuNrExuAu-gjj-fQu4hl-o4y26sqaOMD_tG_S5fvxYPSebt6eX1XKTlKkgOqFSC0NIxiEjhdBZxmVJ0sIKZuUOioJrJqlQpaWpBE6A8RQ4K6WxkozhduwGJZNvGXyMAWzeBdeYMOSU5Md-8qmfnNHxokd-PfHfsTcVnGkTelfWcKKpVvKoYNMYhWeg3JuQQzsa3U9G1vjcVMHF_PVdZ2MUwdk_cU59MQ</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Kamei, M. 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(Kanagawa-ken. Livestock Experiment Station, Ebina (Japan))</creatorcontrib><creatorcontrib>Iwamura, S</creatorcontrib><creatorcontrib>Yoshioka, K</creatorcontrib><creatorcontrib>Kamomae, H</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Japanese Journal of Swine Science (Japan)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamei, M. (Kanagawa-ken. Livestock Experiment Station, Ebina (Japan))</au><au>Iwamura, S</au><au>Yoshioka, K</au><au>Kamomae, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of enzyme immunoassay kits for the determination of pig plasma progesterone levels</atitle><jtitle>Japanese Journal of Swine Science (Japan)</jtitle><addtitle>Nihon Yoton Gakkaishi</addtitle><date>1994</date><risdate>1994</risdate><volume>31</volume><issue>3</issue><spage>109</spage><epage>115</epage><pages>109-115</pages><issn>0913-882X</issn><eissn>1881-655X</eissn><abstract>Enzyme immunoassay kits, Ovucheck Plasma/Serum Progesterone EIA Kit (EIA, Cambridge Veterinary Science Ltd) and Ovucheck Cow Side W (Cow Side W, Cambridge Veterinary Science Ltd), were applied to determination of pigs plasma progesterone (P) levels. We tried to study the modification of EIA assay system for pig P measurement; ie., examined for best dilution methodes with castrated serum/plasma, boar/sow serum and normal saline as dilution solution with 2, 5 and 10 times dilution. All solutions have no effect on the plasma P levels, but castrated serum/plasma and boar serum showed much more suitable for plasma dilution in 2 and 5 times dilution. Porcine plasma P levels assyed by EIA with and without dilution showed significant positive correlation with those by RIA. Cow Side W is used as a qualitative assay system, but it seems to be usable for a semiquantitative assay when using known value solution as standard. Plasma dilution with pigs serum have no effect on Cow Side W assay system too. We demonstrated that modification in assay methods of commercial EIA kits developed for cow were applicable for the determination of pig plasma P levels. Then these system would supply a suitable tool for the diagnosis of pregnancy and ovarian function in pigs.</abstract><pub>The Japanese Society of Swine Science</pub><doi>10.5938/youton.31.109</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0913-882X |
| ispartof | Nihon Yoton Gakkaishi, 1994/09/16, Vol.31(3), pp.109-115 |
| issn | 0913-882X 1881-655X |
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| source | J-STAGE Free; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | ANALYTICAL METHODS BLOOD PLASMA CERDO enzyme immunoassay EQUIPO IMMUNOENZYME TECHNIQUES MATERIEL Ovucheck EIA Kit PLASMA SANGUIN PLASMA SANGUINEO PORCIN progesteron PROGESTERONA PROGESTERONE radio immunoassay SWINE TECHNIQUE ANALYTIQUE TECHNIQUE IMMUNOENZYMATIQUE TECNICAS ANALITICAS TECNICAS INMUNOENZIMATICAS |
| title | Application of enzyme immunoassay kits for the determination of pig plasma progesterone levels |
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