Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B
Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selecti...
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| Veröffentlicht in: | Journal of Oleo Science 2016, Vol.65(11), pp.923-928 |
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| container_issue | 11 |
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| container_title | Journal of Oleo Science |
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| creator | Hori, Ryuji Sano, Takashi Imagi, Jun Watanabe, Yomi |
| description | Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG. |
| doi_str_mv | 10.5650/jos.ess16106 |
| format | Article |
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Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. 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Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG.</description><subject>2-monoacylglycerol</subject><subject>Candida antarctica lipase B</subject><subject>crude oil</subject><subject>phospholipids</subject><subject>regiospecific distribution</subject><subject>triacylglycerol</subject><issn>1345-8957</issn><issn>1347-3352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNo9kNtO4zAQhiMEEsc7HmAeYMPGce20l91sYZGK4ILd22hsj1tXqV3ZRqvwTrwjKQVu5vjNr9FfFNesuhFSVD83Id1QSkyySh4VZ4xPmpJzUR9_1KKczkRzWpyntKmqcS6as-JtYS3pnCBYeHA-RGjDdhc8-cOsjS-G4B-tKKPqCR5dD8FDXhMs_Ouwxew0PFBeBwM5wNxjP7wSPIXksgtjB7eY8wBz7Qz8dilHp172mwTOw3N0qId-1Q-aYugT_Hd53aI3ziCgzxj1qI-wdDtMBL8uixOLfaKrz3xR_L1dPLd_yuXj3X07X5a65kKWZImriTEKTSVrNbUT0lZapSrkaGomSBs0zVQaPZWqViMuOM7sjNW8ISX4RfHjoKtjSCmS7XbRbTEOHau6vdXdaHX3ZfWItwd8kzKu6BvGOH7f0wcsRcfYPn5dfW_1GmNHnr8DkmyRJw</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Hori, Ryuji</creator><creator>Sano, Takashi</creator><creator>Imagi, Jun</creator><creator>Watanabe, Yomi</creator><general>Japan Oil Chemists' Society</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2016</creationdate><title>Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B</title><author>Hori, Ryuji ; Sano, Takashi ; Imagi, Jun ; Watanabe, Yomi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2356-efe3b4ddbad062b8f4ecf6fbb0a3ad215ecdad786dc86b2bfe353a9f91237eb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>2-monoacylglycerol</topic><topic>Candida antarctica lipase B</topic><topic>crude oil</topic><topic>phospholipids</topic><topic>regiospecific distribution</topic><topic>triacylglycerol</topic><toplevel>online_resources</toplevel><creatorcontrib>Hori, Ryuji</creatorcontrib><creatorcontrib>Sano, Takashi</creatorcontrib><creatorcontrib>Imagi, Jun</creatorcontrib><creatorcontrib>Watanabe, Yomi</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Oleo Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hori, Ryuji</au><au>Sano, Takashi</au><au>Imagi, Jun</au><au>Watanabe, Yomi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B</atitle><jtitle>Journal of Oleo Science</jtitle><date>2016</date><risdate>2016</risdate><volume>65</volume><issue>11</issue><spage>923</spage><epage>928</epage><pages>923-928</pages><issn>1345-8957</issn><eissn>1347-3352</eissn><abstract>Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG.</abstract><pub>Japan Oil Chemists' Society</pub><doi>10.5650/jos.ess16106</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 2-monoacylglycerol Candida antarctica lipase B crude oil phospholipids regiospecific distribution triacylglycerol |
| title | Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols withCandida antarctica Lipase B |
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