The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis
BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated good perform...
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| Veröffentlicht in: | The international journal of tuberculosis and lung disease 2015-12, Vol.19 (12), p.1547-1552 |
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| creator | Ardizzoni, E. Mulders, W. Kotrikadze, T. Aspindzelashvili, R. Goginashvili, L. Pangtey, H. Varaine, F. Bastard, M. Rigouts, L. de Jong, B. C. |
| description | BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated
good performance for INH and RMP; however, evidence is still limited, and its applicability for DST of ofloxacin (OFX) and kanamycin (KM) is unknown.DESIGN: We compared 279 TLA DST results with those of MGIT for INH and RMP, and 280 results for OFX and KM with those of the 7H11 agar proportion
method, obtained from 320 smear-positive samples from 165 Georgian TB patients. Discrepancies were solved by comparison with a composite reference standard. The prevalence of multidrug-resistant tuberculosis (TB) was 30 of 164 patients (18.3%), 2 (6.7%) of whom had extensively drug-resistant
TB.RESULTS: TLA showed 94.7%, 98.2%, 100% and 78.9% sensitivity, respectively, for INH, RMP, OFX and KM, with 100% specificity. Average time to results was 7 days in TLA, 23 in MGIT and 49 for 7H11 agar.CONCLUSIONS: In low-resource settings, TLA can be applied for the rapid detection
of resistance to INH, RMP and fluoroquinolones. Further studies are necessary to improve sensitivity to KM and further assess its performance for OFX and other drugs and its applicability in field conditions. |
| doi_str_mv | 10.5588/ijtld.15.0136 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_5588_ijtld_15_0136</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><ingid>iuatld/ijtld/2015/00000019/00000012/art00024</ingid><sourcerecordid>1737480707</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-ea3f774cbefc8dea4ed2574512b70d52deb07b1a4b062992c28bb75184b487053</originalsourceid><addsrcrecordid>eNqFkU2L1DAYgIso7rp69Co5eumYpEmTHmX9hAVB1nPIx9uZDG0yJumy4683Mx29ibnk5eXhIeRpmtcEbziX8p3fl8ltCN9g0vVPmmsiCW_FQPHTOmMq2k6Q4ap5kfMeY0oIEc-bK9r3hFGMr5vD_Q5Q2fnQTvoICemtTmiGsosOjTEh5xPYgg47CLEcD94iB6VufAwojmhepuJbpIND8FggZP8A0xG5tGzbBNnnokNBZTGQ7DLFunjZPBv1lOHV5b5pfnz6eH_7pb379vnr7fu71rKelhZ0NwrBrIHRSgeagaNcME6oEdhx6sBgYYhmBvd0GKil0hjBiWSGSYF5d9O8Xb2HFH8ukIuafbYwTTpAXLIiEktBMO_x_1HRCSaxwKKi7YraFHNOMKpD8rNOR0WwOvVQ5x6KcHXqUfk3F_ViZnB_6T8BKvB9BXzYQiha7eOSQv0Y5a3yiz7LasZTRfVAhkCoorUjlpQrwvigHIy6VlBFJ7X9pTJlVfrhX9LVuD6S4tMzz6fKLwNVOpU6VM1vWiC55A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1737480707</pqid></control><display><type>article</type><title>The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Ardizzoni, E. ; Mulders, W. ; Kotrikadze, T. ; Aspindzelashvili, R. ; Goginashvili, L. ; Pangtey, H. ; Varaine, F. ; Bastard, M. ; Rigouts, L. ; de Jong, B. C.</creator><creatorcontrib>Ardizzoni, E. ; Mulders, W. ; Kotrikadze, T. ; Aspindzelashvili, R. ; Goginashvili, L. ; Pangtey, H. ; Varaine, F. ; Bastard, M. ; Rigouts, L. ; de Jong, B. C.</creatorcontrib><description>BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated
good performance for INH and RMP; however, evidence is still limited, and its applicability for DST of ofloxacin (OFX) and kanamycin (KM) is unknown.DESIGN: We compared 279 TLA DST results with those of MGIT for INH and RMP, and 280 results for OFX and KM with those of the 7H11 agar proportion
method, obtained from 320 smear-positive samples from 165 Georgian TB patients. Discrepancies were solved by comparison with a composite reference standard. The prevalence of multidrug-resistant tuberculosis (TB) was 30 of 164 patients (18.3%), 2 (6.7%) of whom had extensively drug-resistant
TB.RESULTS: TLA showed 94.7%, 98.2%, 100% and 78.9% sensitivity, respectively, for INH, RMP, OFX and KM, with 100% specificity. Average time to results was 7 days in TLA, 23 in MGIT and 49 for 7H11 agar.CONCLUSIONS: In low-resource settings, TLA can be applied for the rapid detection
of resistance to INH, RMP and fluoroquinolones. Further studies are necessary to improve sensitivity to KM and further assess its performance for OFX and other drugs and its applicability in field conditions.</description><identifier>ISSN: 1027-3719</identifier><identifier>EISSN: 1815-7920</identifier><identifier>DOI: 10.5588/ijtld.15.0136</identifier><identifier>PMID: 26614200</identifier><language>eng</language><publisher>France: International Union Against Tuberculosis and Lung Disease</publisher><subject>Antitubercular Agents - pharmacology ; Drug Resistance, Multiple, Bacterial - genetics ; Fluoroquinolones - pharmacology ; Humans ; Isoniazid - pharmacology ; Kanamycin - pharmacology ; Mdr-Tb ; Microbial Sensitivity Tests - methods ; Mycobacterium ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - genetics ; Non-Commercial Method ; Ofloxacin - pharmacology ; Resistance Detection ; Rifampin - pharmacology ; Sensitivity and Specificity ; Sputum - microbiology ; Tuberculosis ; Xdr-Tb</subject><ispartof>The international journal of tuberculosis and lung disease, 2015-12, Vol.19 (12), p.1547-1552</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-ea3f774cbefc8dea4ed2574512b70d52deb07b1a4b062992c28bb75184b487053</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26614200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ardizzoni, E.</creatorcontrib><creatorcontrib>Mulders, W.</creatorcontrib><creatorcontrib>Kotrikadze, T.</creatorcontrib><creatorcontrib>Aspindzelashvili, R.</creatorcontrib><creatorcontrib>Goginashvili, L.</creatorcontrib><creatorcontrib>Pangtey, H.</creatorcontrib><creatorcontrib>Varaine, F.</creatorcontrib><creatorcontrib>Bastard, M.</creatorcontrib><creatorcontrib>Rigouts, L.</creatorcontrib><creatorcontrib>de Jong, B. C.</creatorcontrib><title>The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis</title><title>The international journal of tuberculosis and lung disease</title><addtitle>Int J Tuberc Lung Dis</addtitle><description>BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated
good performance for INH and RMP; however, evidence is still limited, and its applicability for DST of ofloxacin (OFX) and kanamycin (KM) is unknown.DESIGN: We compared 279 TLA DST results with those of MGIT for INH and RMP, and 280 results for OFX and KM with those of the 7H11 agar proportion
method, obtained from 320 smear-positive samples from 165 Georgian TB patients. Discrepancies were solved by comparison with a composite reference standard. The prevalence of multidrug-resistant tuberculosis (TB) was 30 of 164 patients (18.3%), 2 (6.7%) of whom had extensively drug-resistant
TB.RESULTS: TLA showed 94.7%, 98.2%, 100% and 78.9% sensitivity, respectively, for INH, RMP, OFX and KM, with 100% specificity. Average time to results was 7 days in TLA, 23 in MGIT and 49 for 7H11 agar.CONCLUSIONS: In low-resource settings, TLA can be applied for the rapid detection
of resistance to INH, RMP and fluoroquinolones. Further studies are necessary to improve sensitivity to KM and further assess its performance for OFX and other drugs and its applicability in field conditions.</description><subject>Antitubercular Agents - pharmacology</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Fluoroquinolones - pharmacology</subject><subject>Humans</subject><subject>Isoniazid - pharmacology</subject><subject>Kanamycin - pharmacology</subject><subject>Mdr-Tb</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Mycobacterium</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Non-Commercial Method</subject><subject>Ofloxacin - pharmacology</subject><subject>Resistance Detection</subject><subject>Rifampin - pharmacology</subject><subject>Sensitivity and Specificity</subject><subject>Sputum - microbiology</subject><subject>Tuberculosis</subject><subject>Xdr-Tb</subject><issn>1027-3719</issn><issn>1815-7920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1DAYgIso7rp69Co5eumYpEmTHmX9hAVB1nPIx9uZDG0yJumy4683Mx29ibnk5eXhIeRpmtcEbziX8p3fl8ltCN9g0vVPmmsiCW_FQPHTOmMq2k6Q4ap5kfMeY0oIEc-bK9r3hFGMr5vD_Q5Q2fnQTvoICemtTmiGsosOjTEh5xPYgg47CLEcD94iB6VufAwojmhepuJbpIND8FggZP8A0xG5tGzbBNnnokNBZTGQ7DLFunjZPBv1lOHV5b5pfnz6eH_7pb379vnr7fu71rKelhZ0NwrBrIHRSgeagaNcME6oEdhx6sBgYYhmBvd0GKil0hjBiWSGSYF5d9O8Xb2HFH8ukIuafbYwTTpAXLIiEktBMO_x_1HRCSaxwKKi7YraFHNOMKpD8rNOR0WwOvVQ5x6KcHXqUfk3F_ViZnB_6T8BKvB9BXzYQiha7eOSQv0Y5a3yiz7LasZTRfVAhkCoorUjlpQrwvigHIy6VlBFJ7X9pTJlVfrhX9LVuD6S4tMzz6fKLwNVOpU6VM1vWiC55A</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Ardizzoni, E.</creator><creator>Mulders, W.</creator><creator>Kotrikadze, T.</creator><creator>Aspindzelashvili, R.</creator><creator>Goginashvili, L.</creator><creator>Pangtey, H.</creator><creator>Varaine, F.</creator><creator>Bastard, M.</creator><creator>Rigouts, L.</creator><creator>de Jong, B. C.</creator><general>International Union Against Tuberculosis and Lung Disease</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20151201</creationdate><title>The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis</title><author>Ardizzoni, E. ; Mulders, W. ; Kotrikadze, T. ; Aspindzelashvili, R. ; Goginashvili, L. ; Pangtey, H. ; Varaine, F. ; Bastard, M. ; Rigouts, L. ; de Jong, B. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-ea3f774cbefc8dea4ed2574512b70d52deb07b1a4b062992c28bb75184b487053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antitubercular Agents - pharmacology</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Fluoroquinolones - pharmacology</topic><topic>Humans</topic><topic>Isoniazid - pharmacology</topic><topic>Kanamycin - pharmacology</topic><topic>Mdr-Tb</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Mycobacterium</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Non-Commercial Method</topic><topic>Ofloxacin - pharmacology</topic><topic>Resistance Detection</topic><topic>Rifampin - pharmacology</topic><topic>Sensitivity and Specificity</topic><topic>Sputum - microbiology</topic><topic>Tuberculosis</topic><topic>Xdr-Tb</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ardizzoni, E.</creatorcontrib><creatorcontrib>Mulders, W.</creatorcontrib><creatorcontrib>Kotrikadze, T.</creatorcontrib><creatorcontrib>Aspindzelashvili, R.</creatorcontrib><creatorcontrib>Goginashvili, L.</creatorcontrib><creatorcontrib>Pangtey, H.</creatorcontrib><creatorcontrib>Varaine, F.</creatorcontrib><creatorcontrib>Bastard, M.</creatorcontrib><creatorcontrib>Rigouts, L.</creatorcontrib><creatorcontrib>de Jong, B. C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>The international journal of tuberculosis and lung disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ardizzoni, E.</au><au>Mulders, W.</au><au>Kotrikadze, T.</au><au>Aspindzelashvili, R.</au><au>Goginashvili, L.</au><au>Pangtey, H.</au><au>Varaine, F.</au><au>Bastard, M.</au><au>Rigouts, L.</au><au>de Jong, B. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis</atitle><jtitle>The international journal of tuberculosis and lung disease</jtitle><addtitle>Int J Tuberc Lung Dis</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>19</volume><issue>12</issue><spage>1547</spage><epage>1552</epage><pages>1547-1552</pages><issn>1027-3719</issn><eissn>1815-7920</eissn><abstract>BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated
good performance for INH and RMP; however, evidence is still limited, and its applicability for DST of ofloxacin (OFX) and kanamycin (KM) is unknown.DESIGN: We compared 279 TLA DST results with those of MGIT for INH and RMP, and 280 results for OFX and KM with those of the 7H11 agar proportion
method, obtained from 320 smear-positive samples from 165 Georgian TB patients. Discrepancies were solved by comparison with a composite reference standard. The prevalence of multidrug-resistant tuberculosis (TB) was 30 of 164 patients (18.3%), 2 (6.7%) of whom had extensively drug-resistant
TB.RESULTS: TLA showed 94.7%, 98.2%, 100% and 78.9% sensitivity, respectively, for INH, RMP, OFX and KM, with 100% specificity. Average time to results was 7 days in TLA, 23 in MGIT and 49 for 7H11 agar.CONCLUSIONS: In low-resource settings, TLA can be applied for the rapid detection
of resistance to INH, RMP and fluoroquinolones. Further studies are necessary to improve sensitivity to KM and further assess its performance for OFX and other drugs and its applicability in field conditions.</abstract><cop>France</cop><pub>International Union Against Tuberculosis and Lung Disease</pub><pmid>26614200</pmid><doi>10.5588/ijtld.15.0136</doi><tpages>6</tpages></addata></record> |
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| subjects | Antitubercular Agents - pharmacology Drug Resistance, Multiple, Bacterial - genetics Fluoroquinolones - pharmacology Humans Isoniazid - pharmacology Kanamycin - pharmacology Mdr-Tb Microbial Sensitivity Tests - methods Mycobacterium Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Non-Commercial Method Ofloxacin - pharmacology Resistance Detection Rifampin - pharmacology Sensitivity and Specificity Sputum - microbiology Tuberculosis Xdr-Tb |
| title | The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis |
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