MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro
In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and...
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| Veröffentlicht in: | Acta neurobiologiae experimentalis 2004-01, Vol.64 (4), p.461-466 |
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| container_title | Acta neurobiologiae experimentalis |
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| creator | Kabadere, Selda Oztopçu, Pinar Korkmaz, Seval Erol, Kevser Uyar, Ruhi |
| description | In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells. |
| doi_str_mv | 10.55782/ane-2004-1528 |
| format | Article |
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C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.</description><identifier>ISSN: 0065-1400</identifier><identifier>EISSN: 1689-0035</identifier><identifier>DOI: 10.55782/ane-2004-1528</identifier><identifier>PMID: 15586662</identifier><language>eng</language><publisher>Poland</publisher><subject>Animals ; Antioxidants - pharmacology ; Calcium Channel Blockers - pharmacology ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Chromans - pharmacology ; Dose-Response Relationship, Drug ; Drug Interactions ; Glioma ; Glutamic Acid - pharmacology ; Humans ; Inhibitory Concentration 50 ; Magnesium Sulfate - pharmacology ; Mice ; Neuroglia - cytology ; Neuroglia - drug effects ; Piperazines - pharmacology ; Tetrazolium Salts ; Thiazoles</subject><ispartof>Acta neurobiologiae experimentalis, 2004-01, Vol.64 (4), p.461-466</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c289t-6556e9ad162d094bf7539658d676049df7dbf7f80893c69a3ec5f8f044d854133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15586662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kabadere, Selda</creatorcontrib><creatorcontrib>Oztopçu, Pinar</creatorcontrib><creatorcontrib>Korkmaz, Seval</creatorcontrib><creatorcontrib>Erol, Kevser</creatorcontrib><creatorcontrib>Uyar, Ruhi</creatorcontrib><title>MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro</title><title>Acta neurobiologiae experimentalis</title><addtitle>Acta Neurobiol Exp (Wars)</addtitle><description>In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chromans - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Interactions</subject><subject>Glioma</subject><subject>Glutamic Acid - pharmacology</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Magnesium Sulfate - pharmacology</subject><subject>Mice</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - drug effects</subject><subject>Piperazines - pharmacology</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><issn>0065-1400</issn><issn>1689-0035</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0EtLAzEUBeAgiq3VrUvJUhepN89JliL1AZUutOshnWRKZB4lScX6653agqsLh3vO4kPomsJUykKze9t5wgAEoZLpEzSmShsCwOUpGgMoSagAGKGLlD4BWFEwOEcjKqVWSrExsm_r94XAtnO4sT829sHh2yXRXHM1u8MbG3OwTbPDm9hnX-WE103oW4sr3zQJ27UNXcpDuM22tdnj3H-HKuQdDh3-Cjn2l-istk3yV8c7Qcun2cfjC5kvnl8fH-akYtpkoqRU3lhHFXNgxKouJDdKaqcKBcK4unBDVmvQhlfKWO4rWesahHBaCsr5BE0Pu1XsU4q-LjcxtDbuSgrln1U5WJV7q3JvNRRuDoXNdtV69_9-xOG_2TNkJw</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Kabadere, Selda</creator><creator>Oztopçu, Pinar</creator><creator>Korkmaz, Seval</creator><creator>Erol, Kevser</creator><creator>Uyar, Ruhi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20040101</creationdate><title>MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro</title><author>Kabadere, Selda ; Oztopçu, Pinar ; Korkmaz, Seval ; Erol, Kevser ; Uyar, Ruhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-6556e9ad162d094bf7539658d676049df7dbf7f80893c69a3ec5f8f044d854133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Chromans - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Interactions</topic><topic>Glioma</topic><topic>Glutamic Acid - pharmacology</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>Magnesium Sulfate - pharmacology</topic><topic>Mice</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - drug effects</topic><topic>Piperazines - pharmacology</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kabadere, Selda</creatorcontrib><creatorcontrib>Oztopçu, Pinar</creatorcontrib><creatorcontrib>Korkmaz, Seval</creatorcontrib><creatorcontrib>Erol, Kevser</creatorcontrib><creatorcontrib>Uyar, Ruhi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Acta neurobiologiae experimentalis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kabadere, Selda</au><au>Oztopçu, Pinar</au><au>Korkmaz, Seval</au><au>Erol, Kevser</au><au>Uyar, Ruhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro</atitle><jtitle>Acta neurobiologiae experimentalis</jtitle><addtitle>Acta Neurobiol Exp (Wars)</addtitle><date>2004-01-01</date><risdate>2004</risdate><volume>64</volume><issue>4</issue><spage>461</spage><epage>466</epage><pages>461-466</pages><issn>0065-1400</issn><eissn>1689-0035</eissn><abstract>In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.</abstract><cop>Poland</cop><pmid>15586662</pmid><doi>10.55782/ane-2004-1528</doi><tpages>6</tpages></addata></record> |
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| ispartof | Acta neurobiologiae experimentalis, 2004-01, Vol.64 (4), p.461-466 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antioxidants - pharmacology Calcium Channel Blockers - pharmacology Cell Line, Tumor Cell Proliferation - drug effects Cell Survival - drug effects Chromans - pharmacology Dose-Response Relationship, Drug Drug Interactions Glioma Glutamic Acid - pharmacology Humans Inhibitory Concentration 50 Magnesium Sulfate - pharmacology Mice Neuroglia - cytology Neuroglia - drug effects Piperazines - pharmacology Tetrazolium Salts Thiazoles |
| title | MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro |
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