Lack of glyphosate resistance gene transfer from Roundup Ready soybean to Bradyrhizobium japonicum under field and laboratory conditions

A field study was conducted at the Russell E. Larson Agricultural Research Center to determine the effect of transgenic glyphosate-resistant soybean in combination with herbicide (Roundup) application on its endosymbiont Bradyrhizobium japonicum. DNA of bacteroids from isolated nodules was analysed...

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Veröffentlicht in:Zeitschrift für Naturforschung C. A journal of biosciences 2011-11, Vol.66 (11-12), p.595
Hauptverfasser: Isaza, Laura Arango, Opelt, Katja, Wagner, Tobias, Mattes, Elke, Bieber, Evi, Hatley, Elwood O, Roth, Greg, Sanjuán, Juan, Fischer, Hans-Martin, Sandermann, Heinrich, Hartmann, Anton, Ernst, Dieter
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container_issue 11-12
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container_title Zeitschrift für Naturforschung C. A journal of biosciences
container_volume 66
creator Isaza, Laura Arango
Opelt, Katja
Wagner, Tobias
Mattes, Elke
Bieber, Evi
Hatley, Elwood O
Roth, Greg
Sanjuán, Juan
Fischer, Hans-Martin
Sandermann, Heinrich
Hartmann, Anton
Ernst, Dieter
description A field study was conducted at the Russell E. Larson Agricultural Research Center to determine the effect of transgenic glyphosate-resistant soybean in combination with herbicide (Roundup) application on its endosymbiont Bradyrhizobium japonicum. DNA of bacteroids from isolated nodules was analysed for the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) DNA sequence using polymerase chain reaction (PCR). To further assess the likelihood that the EPSPS gene may be transferred from the Roundup Ready (RR) soybean to B. japonicum, we have examined the natural transformation efficiency of B. japonicum strain 110spc4. Analyses of nodules showed the presence of the transgenic EPSPS DNA sequence. In bacteroids that were isolated from nodules of transgenic soybean plants and then cultivated in the presence of glyphosate this sequence could not be detected. This indicates that no stable horizontal gene transfer (HGT) of the EPSPS gene had occurred under field conditions. Under laboratory conditions, no natural transformation was detected in B. japonicum strain 110spc4 in the presence of various amounts of recombinant plasmid DNA. Our results indicate that no natural competence state exists in B. japonicum 110spc4. Results from field and laboratory studies indicate the lack of functional transfer of the CP4-EPSPS gene from glyphosate-tolerant soybean treated with glyphosate to root-associated B. japonicum.
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subjects Base Sequence
Bradyrhizobium - genetics
DNA Primers
Gene Transfer Techniques
Glycine - analogs & derivatives
Glycine - pharmacology
Glycine max - genetics
Glyphosate
Insecticide Resistance - genetics
Plants, Genetically Modified
Polymerase Chain Reaction
title Lack of glyphosate resistance gene transfer from Roundup Ready soybean to Bradyrhizobium japonicum under field and laboratory conditions
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