A Straightforward Method to Scale-Up Plasmid DNA Intermediate Recovery by Tangential Flow Ultrafiltration
The interest in gene therapy and production of vaccines based on plasmid DNA (pDNA) has increased in recent years because they are novel techniques for the treatment or prevention of genetic diseases or infections. A typical bioprocess for the production of pDNA includes four stages: fermentation, p...
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| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2013, Vol.27 (4), p.4014-4017 |
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| container_title | Biotechnology, biotechnological equipment |
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| creator | Figueroa-Rosette, Julieth Alejandra Guerrero-Germán, Patricia Montesinos-Cisneros, Rosa María Mártin-García, Abraham Rogelio Tejeda-Mansir, Armando |
| description | The interest in gene therapy and production of vaccines based on plasmid DNA (pDNA) has increased in recent years because they are novel techniques for the treatment or prevention of genetic diseases or infections. A typical bioprocess for the production of pDNA includes four stages: fermentation, primary recovery intermediate recovery, and final purification. The crucial stage in the process is the capture of pDNA, as it is essential to provide good quality material for the purification stage. In this work, the behavior of tangential flow ultrafiltration (TFUF) in the intermediate recovery of plasmid pVAX1-LipL32 from lysates of E. coli ferments was investigated as an alternative to recovery operations that use chemical agents harmful to the environment. The concentration profile of the nucleic acids in both filtrate and retentate were determined by global analysis. The plasmid integrity during the recovery stages was verified at several intervals by means of electrophoresis analysis. A simple mathematical model was obtained to describe the process behavior that can be used as a straightforward method for the scale-up of the recovery process. |
| doi_str_mv | 10.5504/BBEQ.2013.0053 |
| format | Article |
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A simple mathematical model was obtained to describe the process behavior that can be used as a straightforward method for the scale-up of the recovery process.</description><identifier>ISSN: 1310-2818</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.5504/BBEQ.2013.0053</identifier><language>eng</language><publisher>Sofia: Taylor & Francis</publisher><subject>bioprocess ; Chemical agents ; Deoxyribonucleic acid ; DNA ; DNA vaccines ; E coli ; Electrophoresis ; Escherichia coli ; Fermentation ; Filtrate ; Gene therapy ; intermediate recovery ; Lysates ; Mathematical models ; Medical treatment ; Nucleic acids ; plasmid DNA ; Plasmids ; Purification ; Recovery ; Ultrafiltration ; Vaccines</subject><ispartof>Biotechnology, biotechnological equipment, 2013, Vol.27 (4), p.4014-4017</ispartof><rights>2013 Taylor and Francis Group, LLC 2013</rights><rights>2013 Taylor and Francis Group, LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c305t-d1a0827c2e3bb1a225188c15acb749340162fe61dfc4d963076b4eb5cbd44e9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids></links><search><creatorcontrib>Figueroa-Rosette, Julieth Alejandra</creatorcontrib><creatorcontrib>Guerrero-Germán, Patricia</creatorcontrib><creatorcontrib>Montesinos-Cisneros, Rosa María</creatorcontrib><creatorcontrib>Mártin-García, Abraham Rogelio</creatorcontrib><creatorcontrib>Tejeda-Mansir, Armando</creatorcontrib><title>A Straightforward Method to Scale-Up Plasmid DNA Intermediate Recovery by Tangential Flow Ultrafiltration</title><title>Biotechnology, biotechnological equipment</title><description>The interest in gene therapy and production of vaccines based on plasmid DNA (pDNA) has increased in recent years because they are novel techniques for the treatment or prevention of genetic diseases or infections. 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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | bioprocess Chemical agents Deoxyribonucleic acid DNA DNA vaccines E coli Electrophoresis Escherichia coli Fermentation Filtrate Gene therapy intermediate recovery Lysates Mathematical models Medical treatment Nucleic acids plasmid DNA Plasmids Purification Recovery Ultrafiltration Vaccines |
| title | A Straightforward Method to Scale-Up Plasmid DNA Intermediate Recovery by Tangential Flow Ultrafiltration |
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