Characterization of glucans and glucansucrases from novel Leuconostoc strains (including sp. S-51)
Nine kinds of glucan-producing strains were isolated in their culture supernatant from the lines of a sugar-manufacturing factory. Eight strains produced water-soluble glucan.13C NMR analysis suggested that five out of the nine glucans mainly consisted of 6-Glcp and the other four glucans were branc...
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| Veröffentlicht in: | Journal of Applied Glycoscience 2003/07/20, Vol.50(3), pp.379-382 |
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| container_title | Journal of Applied Glycoscience |
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| creator | Funane, K. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) Matsuo, T Ono, H Ishii, T Gibu, S Tokashiki, T Kobayashi, M |
| description | Nine kinds of glucan-producing strains were isolated in their culture supernatant from the lines of a sugar-manufacturing factory. Eight strains produced water-soluble glucan.13C NMR analysis suggested that five out of the nine glucans mainly consisted of 6-Glcp and the other four glucans were branched. Among the 6-Glcp glucan-producing strains, S-51 was selected because it had the highest extracellular glucansucrase activity. S-51 strain was identified as Leuconostoc sp. Only one kind of glucansucrase with a molecular mass of 165 kDa was found in the culture supernatant of strain S-51. Optimum temperature and pH of the S-51 glucansucrase were 35°C and 5.5, respectively. Compared with the B-512F glucansucrase, S-51 glucansucrase has a lower Km value and was slightly more stable at varying pHs and temperatures. |
| doi_str_mv | 10.5458/jag.50.379 |
| format | Article |
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S-51)</title><source>J-STAGE Free</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Funane, K. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) ; Matsuo, T ; Ono, H ; Ishii, T ; Gibu, S ; Tokashiki, T ; Kobayashi, M</creator><creatorcontrib>Funane, K. (National Food Research Inst., Tsukuba, Ibaraki (Japan)) ; Matsuo, T ; Ono, H ; Ishii, T ; Gibu, S ; Tokashiki, T ; Kobayashi, M</creatorcontrib><description>Nine kinds of glucan-producing strains were isolated in their culture supernatant from the lines of a sugar-manufacturing factory. Eight strains produced water-soluble glucan.13C NMR analysis suggested that five out of the nine glucans mainly consisted of 6-Glcp and the other four glucans were branched. Among the 6-Glcp glucan-producing strains, S-51 was selected because it had the highest extracellular glucansucrase activity. S-51 strain was identified as Leuconostoc sp. Only one kind of glucansucrase with a molecular mass of 165 kDa was found in the culture supernatant of strain S-51. Optimum temperature and pH of the S-51 glucansucrase were 35°C and 5.5, respectively. Compared with the B-512F glucansucrase, S-51 glucansucrase has a lower Km value and was slightly more stable at varying pHs and temperatures.</description><identifier>ISSN: 1344-7882</identifier><identifier>EISSN: 1880-7291</identifier><identifier>DOI: 10.5458/jag.50.379</identifier><language>eng</language><publisher>The Japanese Society of Applied Glycoscience</publisher><subject>DEXTRANS ; glucan, glucansucrase, dextran, dextransucrase ; GLUCANS ; HYDROLASES ; LEUCONOSTOC</subject><ispartof>Journal of Applied Glycoscience, 2003/07/20, Vol.50(3), pp.379-382</ispartof><rights>The Japanese Society of Applied Glycoscience</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2199-356f6cae9cacfad60729180f60d683809e7a19949f14afa0a3381b220ab755ca3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Funane, K. 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S-51 strain was identified as Leuconostoc sp. Only one kind of glucansucrase with a molecular mass of 165 kDa was found in the culture supernatant of strain S-51. Optimum temperature and pH of the S-51 glucansucrase were 35°C and 5.5, respectively. Compared with the B-512F glucansucrase, S-51 glucansucrase has a lower Km value and was slightly more stable at varying pHs and temperatures.</description><subject>DEXTRANS</subject><subject>glucan, glucansucrase, dextran, dextransucrase</subject><subject>GLUCANS</subject><subject>HYDROLASES</subject><subject>LEUCONOSTOC</subject><issn>1344-7882</issn><issn>1880-7291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNo9kE1LxDAQhosouK5evAs5qtA1aZo2OUrxkwUF9Rxm06Rm6SaStIL-erNW9zIzkGfeCU-WnRK8YCXjV2voFgwvaC32shnhHOd1Ich-mmlZ5jXnxWF2FOMa47IivJplq-YdAqhBB_sNg_UOeYO6flTgIgLX_s-jChB1RCb4DXL-U_doqUflnY-DVygOAWzaOLdO9WNrXYfixwK95IxcHGcHBvqoT_76PHu7vXlt7vPl091Dc73MVUGEyCmrTKVACwXKQFvh7c85NhVuK045FrqGxJXCkBIMYKCUk1VRYFjVjCmg8-xyylXBxxi0kR_BbiB8SYLl1o5MdiTDMtlJcDPB6zhAp3cohMGqXm_RdEz84lNJW7tXlaRJ7VLK2ZRiwEvogo3y8blIcjHGBaP0B0APeTM</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Funane, K. 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(National Food Research Inst., Tsukuba, Ibaraki (Japan))</creatorcontrib><creatorcontrib>Matsuo, T</creatorcontrib><creatorcontrib>Ono, H</creatorcontrib><creatorcontrib>Ishii, T</creatorcontrib><creatorcontrib>Gibu, S</creatorcontrib><creatorcontrib>Tokashiki, T</creatorcontrib><creatorcontrib>Kobayashi, M</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Journal of Applied Glycoscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Funane, K. (National Food Research Inst., Tsukuba, Ibaraki (Japan))</au><au>Matsuo, T</au><au>Ono, H</au><au>Ishii, T</au><au>Gibu, S</au><au>Tokashiki, T</au><au>Kobayashi, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of glucans and glucansucrases from novel Leuconostoc strains (including sp. S-51)</atitle><jtitle>Journal of Applied Glycoscience</jtitle><addtitle>J. Appl. Glycosci.</addtitle><date>2003</date><risdate>2003</risdate><volume>50</volume><issue>3</issue><spage>379</spage><epage>382</epage><pages>379-382</pages><issn>1344-7882</issn><eissn>1880-7291</eissn><abstract>Nine kinds of glucan-producing strains were isolated in their culture supernatant from the lines of a sugar-manufacturing factory. Eight strains produced water-soluble glucan.13C NMR analysis suggested that five out of the nine glucans mainly consisted of 6-Glcp and the other four glucans were branched. Among the 6-Glcp glucan-producing strains, S-51 was selected because it had the highest extracellular glucansucrase activity. S-51 strain was identified as Leuconostoc sp. Only one kind of glucansucrase with a molecular mass of 165 kDa was found in the culture supernatant of strain S-51. Optimum temperature and pH of the S-51 glucansucrase were 35°C and 5.5, respectively. Compared with the B-512F glucansucrase, S-51 glucansucrase has a lower Km value and was slightly more stable at varying pHs and temperatures.</abstract><pub>The Japanese Society of Applied Glycoscience</pub><doi>10.5458/jag.50.379</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | DEXTRANS glucan, glucansucrase, dextran, dextransucrase GLUCANS HYDROLASES LEUCONOSTOC |
| title | Characterization of glucans and glucansucrases from novel Leuconostoc strains (including sp. S-51) |
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