Synthesis and Properties of Hetero-branched Cyclodextrins
Hetero-branched cyclodextrins (CDs) were synthesized by transglycosylation or the reverse action of several enzymes such as βββ-galactosidases, α-galactosidases, α-mannosidases, lysozyme and R-Nacetylhexosaminidase. Their structures were analyzed by methylation, FAB-MS, and NMR spectroscopies. β-Gal...
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Veröffentlicht in: | Journal of Applied Glycoscience 1999/06/30, Vol.46(2), pp.217-225 |
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container_title | Journal of Applied Glycoscience |
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creator | Hamayasu, Kenichi Hara, Koji Fujita, Koki Hashimoto, Hitoshi Matsuda, Kazuha Okada, Yasuyo Tanimoto, Toshiko Koizumi, Kyoko Nakano, Hirofumi Kitahata, Sumio |
description | Hetero-branched cyclodextrins (CDs) were synthesized by transglycosylation or the reverse action of several enzymes such as βββ-galactosidases, α-galactosidases, α-mannosidases, lysozyme and R-Nacetylhexosaminidase. Their structures were analyzed by methylation, FAB-MS, and NMR spectroscopies. β-Galactosidase and a-galactosidase from microorganisms synthesized hetero-branched CDs, of which the galactose residues were linked at side chains of the branched CDs. But these enzymes could not synthesize galactosyl-CDs, directly linked to the CD ring. However, α-galactosidase from coffee bean and α-mannosidase and N-acetylhexosaminidase from jack bean could bind galactosyl, mannosyl and N-acetylglucosaminyl residues directly to the CD rings, respectively, by transglycosylation or reverse action. The effects of the side-chain residues in branched CDs, on solubility, hemolytic activity, and inclusion reactions with 6-O-α-D-glucosyl-CDs, 6-O-α-D-galactosyl-CDs, and 6-O-α-Dmannosyl-CDs, were examined. |
doi_str_mv | 10.5458/jag.46.217 |
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Their structures were analyzed by methylation, FAB-MS, and NMR spectroscopies. β-Galactosidase and a-galactosidase from microorganisms synthesized hetero-branched CDs, of which the galactose residues were linked at side chains of the branched CDs. But these enzymes could not synthesize galactosyl-CDs, directly linked to the CD ring. However, α-galactosidase from coffee bean and α-mannosidase and N-acetylhexosaminidase from jack bean could bind galactosyl, mannosyl and N-acetylglucosaminyl residues directly to the CD rings, respectively, by transglycosylation or reverse action. The effects of the side-chain residues in branched CDs, on solubility, hemolytic activity, and inclusion reactions with 6-O-α-D-glucosyl-CDs, 6-O-α-D-galactosyl-CDs, and 6-O-α-Dmannosyl-CDs, were examined.</description><identifier>ISSN: 1344-7882</identifier><identifier>EISSN: 1880-7291</identifier><identifier>DOI: 10.5458/jag.46.217</identifier><language>eng</language><publisher>The Japanese Society of Applied Glycoscience</publisher><ispartof>Journal of Applied Glycoscience, 1999/06/30, Vol.46(2), pp.217-225</ispartof><rights>The Japanese Society of Applied Glycoscience</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids></links><search><creatorcontrib>Hamayasu, Kenichi</creatorcontrib><creatorcontrib>Hara, Koji</creatorcontrib><creatorcontrib>Fujita, Koki</creatorcontrib><creatorcontrib>Hashimoto, Hitoshi</creatorcontrib><creatorcontrib>Matsuda, Kazuha</creatorcontrib><creatorcontrib>Okada, Yasuyo</creatorcontrib><creatorcontrib>Tanimoto, Toshiko</creatorcontrib><creatorcontrib>Koizumi, Kyoko</creatorcontrib><creatorcontrib>Nakano, Hirofumi</creatorcontrib><creatorcontrib>Kitahata, Sumio</creatorcontrib><title>Synthesis and Properties of Hetero-branched Cyclodextrins</title><title>Journal of Applied Glycoscience</title><addtitle>J. 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title | Synthesis and Properties of Hetero-branched Cyclodextrins |
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