Electrochemical Analysis of Guanine Base in the Yeast Cell
The cellular harm or mutation results from the indirect reaction through reactive oxygen species (ROS) generated by ultraviolet (UV) ray or direct reaction (such as cyclobutane pyrimidine dimmer (CPD) and 6-4 photoproduct). The amount of intracellular guanine nucleotide irradiated by the 0-80 J/m2 U...
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| Veröffentlicht in: | Hoken butsuri 2009, Vol.44(1), pp.46-52 |
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| container_title | Hoken butsuri |
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| creator | MATUO, Youichirou YOKOYAMA, Kazuya YASUTOMI, Takuya AKIYAMA, Yoko IZUMI, Yoshinobu SHIMIZU, Kikuo NISHIJIMA, Shigehiro |
| description | The cellular harm or mutation results from the indirect reaction through reactive oxygen species (ROS) generated by ultraviolet (UV) ray or direct reaction (such as cyclobutane pyrimidine dimmer (CPD) and 6-4 photoproduct). The amount of intracellular guanine nucleotide irradiated by the 0-80 J/m2 UV-C (254 nm) was measured by means of cyclic voltammetry (CV). The oxidation current peak of intracellular guanine nucleotide was observed. The current peak which reflects the concentration of intracellular guanine base decreased to about 80% of initial value with 80 J/m2 of UV-irradiation. The concentration of guanine nucleotide could be estimated from the oxidation peak current in CV. Moreover, formation of 8-oxodG was estimated by the increase in the mutation frequency from UV-irradiated cells of base excision repair deficient strain. It was shown that the decrease in the peak current is due to the oxidation of the guanine base. This analytical method is useful for the elucidation of the oxidation mechanism of the cell and biological tissues. |
| doi_str_mv | 10.5453/jhps.44.46 |
| format | Article |
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The amount of intracellular guanine nucleotide irradiated by the 0-80 J/m2 UV-C (254 nm) was measured by means of cyclic voltammetry (CV). The oxidation current peak of intracellular guanine nucleotide was observed. The current peak which reflects the concentration of intracellular guanine base decreased to about 80% of initial value with 80 J/m2 of UV-irradiation. The concentration of guanine nucleotide could be estimated from the oxidation peak current in CV. Moreover, formation of 8-oxodG was estimated by the increase in the mutation frequency from UV-irradiated cells of base excision repair deficient strain. It was shown that the decrease in the peak current is due to the oxidation of the guanine base. This analytical method is useful for the elucidation of the oxidation mechanism of the cell and biological tissues.</description><identifier>ISSN: 0367-6110</identifier><identifier>EISSN: 1884-7560</identifier><identifier>DOI: 10.5453/jhps.44.46</identifier><language>eng</language><publisher>Japan Health Physics Society</publisher><subject>8-oxodG ; Cyclic voltammetry ; GTP ; Guanine ; UV-C</subject><ispartof>Japanese Journal of Health Physics, 2009, Vol.44(1), pp.46-52</ispartof><rights>2009 Japan Health Physics Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1606-71f3b049059ee5a01dd1844eee27d9642229a57e31fbb9506f0d3be565b92c4f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>MATUO, Youichirou</creatorcontrib><creatorcontrib>YOKOYAMA, Kazuya</creatorcontrib><creatorcontrib>YASUTOMI, Takuya</creatorcontrib><creatorcontrib>AKIYAMA, Yoko</creatorcontrib><creatorcontrib>IZUMI, Yoshinobu</creatorcontrib><creatorcontrib>SHIMIZU, Kikuo</creatorcontrib><creatorcontrib>NISHIJIMA, Shigehiro</creatorcontrib><title>Electrochemical Analysis of Guanine Base in the Yeast Cell</title><title>Hoken butsuri</title><addtitle>Jpn. 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This analytical method is useful for the elucidation of the oxidation mechanism of the cell and biological tissues.</description><subject>8-oxodG</subject><subject>Cyclic voltammetry</subject><subject>GTP</subject><subject>Guanine</subject><subject>UV-C</subject><issn>0367-6110</issn><issn>1884-7560</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNo9j01LAzEYhIMoWGov_oKcha1vNh_beJG6tFUoeNGDp5DNvnFT0m1J1kP_vS0tvcwwzMPAEPLIYCqF5M-bbp-nQkyFuiEjNpuJopIKbskIuKoKxRjck0nOoQEAzY9ZjsjLIqIb0s51uA3ORjrvbTzkkOnO09Wf7UOP9M1mpKGnQ4f0B20eaI0xPpA7b2PGycXH5Hu5-Krfi_Xn6qOerwvHFKiiYp43IDRIjSgtsLZlMyEQsaxarURZltrKCjnzTaMlKA8tb1Aq2ejSCc_H5Om869Iu54Te7FPY2nQwDMzpuDkdN0IYoY7w6xne5MH-4hW1aQgu4hVlF1HXxnU2Gez5P6gYYdc</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>MATUO, Youichirou</creator><creator>YOKOYAMA, Kazuya</creator><creator>YASUTOMI, Takuya</creator><creator>AKIYAMA, Yoko</creator><creator>IZUMI, Yoshinobu</creator><creator>SHIMIZU, Kikuo</creator><creator>NISHIJIMA, Shigehiro</creator><general>Japan Health Physics Society</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2009</creationdate><title>Electrochemical Analysis of Guanine Base in the Yeast Cell</title><author>MATUO, Youichirou ; YOKOYAMA, Kazuya ; YASUTOMI, Takuya ; AKIYAMA, Yoko ; IZUMI, Yoshinobu ; SHIMIZU, Kikuo ; NISHIJIMA, Shigehiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1606-71f3b049059ee5a01dd1844eee27d9642229a57e31fbb9506f0d3be565b92c4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>8-oxodG</topic><topic>Cyclic voltammetry</topic><topic>GTP</topic><topic>Guanine</topic><topic>UV-C</topic><toplevel>online_resources</toplevel><creatorcontrib>MATUO, Youichirou</creatorcontrib><creatorcontrib>YOKOYAMA, Kazuya</creatorcontrib><creatorcontrib>YASUTOMI, Takuya</creatorcontrib><creatorcontrib>AKIYAMA, Yoko</creatorcontrib><creatorcontrib>IZUMI, Yoshinobu</creatorcontrib><creatorcontrib>SHIMIZU, Kikuo</creatorcontrib><creatorcontrib>NISHIJIMA, Shigehiro</creatorcontrib><collection>CrossRef</collection><jtitle>Hoken butsuri</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATUO, Youichirou</au><au>YOKOYAMA, Kazuya</au><au>YASUTOMI, Takuya</au><au>AKIYAMA, Yoko</au><au>IZUMI, Yoshinobu</au><au>SHIMIZU, Kikuo</au><au>NISHIJIMA, Shigehiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical Analysis of Guanine Base in the Yeast Cell</atitle><jtitle>Hoken butsuri</jtitle><addtitle>Jpn. 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Moreover, formation of 8-oxodG was estimated by the increase in the mutation frequency from UV-irradiated cells of base excision repair deficient strain. It was shown that the decrease in the peak current is due to the oxidation of the guanine base. This analytical method is useful for the elucidation of the oxidation mechanism of the cell and biological tissues.</abstract><pub>Japan Health Physics Society</pub><doi>10.5453/jhps.44.46</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 8-oxodG Cyclic voltammetry GTP Guanine UV-C |
| title | Electrochemical Analysis of Guanine Base in the Yeast Cell |
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