A surface plasmon resonance-based immunosensor for sensitive detection of bisphenol A
A method for the determination of bisphenol A (BPA), a representative endocrine-disrupting chemicals, was developed using a surfase plasmon resonance (SPR) sensor. BPA-ovalbumin (BPA-OVA) conjugate was immobilized on an Au thin film of the SPR sensor chip by physical adsorption, and BPA determinatio...
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| Veröffentlicht in: | Journal of the Faculty of Agriculture, Kyushu University Kyushu University, 2005-10, Vol.50 (2), p.625-634 |
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| container_title | Journal of the Faculty of Agriculture, Kyushu University |
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| creator | Matsumoto, K.(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) Sakai, T Torimaru, A Ishitobi, S |
| description | A method for the determination of bisphenol A (BPA), a representative endocrine-disrupting chemicals, was developed using a surfase plasmon resonance (SPR) sensor. BPA-ovalbumin (BPA-OVA) conjugate was immobilized on an Au thin film of the SPR sensor chip by physical adsorption, and BPA determinations were performed by an indirect competitive immunoassay, where a BPA sample containing an anti-BPA antibody is introduced into the SPR sensor system. The addition of BPA into the anti-BPA antibody solution (-0.8 mug/final, final concentration) was found to decrease the incidence angle shift sharply because of an inhibition effect of BPA. The RSDs (n=3) of each point were less than 4%. An evaluation of the affinity constant (K1) between anti-BPA antibody and BPA-OVA conjugate on the chip was found to be 2.26 X 10(6) M(-l) and that (K2) between anti-BPA-antibody and free BPA (analyte) was 5.72 X l0(4) M(-1). The lowest detection limit for BPA by SPR was almost the same as that by ELISA, 10(-9)g/ml (1 ppb). |
| doi_str_mv | 10.5109/4676 |
| format | Article |
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Faculty of Agriculture) ; Sakai, T ; Torimaru, A ; Ishitobi, S</creator><creatorcontrib>Matsumoto, K.(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) ; Sakai, T ; Torimaru, A ; Ishitobi, S</creatorcontrib><description>A method for the determination of bisphenol A (BPA), a representative endocrine-disrupting chemicals, was developed using a surfase plasmon resonance (SPR) sensor. BPA-ovalbumin (BPA-OVA) conjugate was immobilized on an Au thin film of the SPR sensor chip by physical adsorption, and BPA determinations were performed by an indirect competitive immunoassay, where a BPA sample containing an anti-BPA antibody is introduced into the SPR sensor system. The addition of BPA into the anti-BPA antibody solution (-0.8 mug/final, final concentration) was found to decrease the incidence angle shift sharply because of an inhibition effect of BPA. The RSDs (n=3) of each point were less than 4%. An evaluation of the affinity constant (K1) between anti-BPA antibody and BPA-OVA conjugate on the chip was found to be 2.26 X 10(6) M(-l) and that (K2) between anti-BPA-antibody and free BPA (analyte) was 5.72 X l0(4) M(-1). 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The RSDs (n=3) of each point were less than 4%. An evaluation of the affinity constant (K1) between anti-BPA antibody and BPA-OVA conjugate on the chip was found to be 2.26 X 10(6) M(-l) and that (K2) between anti-BPA-antibody and free BPA (analyte) was 5.72 X l0(4) M(-1). 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| ispartof | Journal of the Faculty of Agriculture, Kyushu University, 2005-10, Vol.50 (2), p.625-634 |
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| source | Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals |
| subjects | BIODETECTEUR BIOSENSORES BIOSENSORS COMPOSE PHENOLIQUE COMPUESTOS FENOLICOS ELISA GENERO HUMANO GENRE HUMAIN IMMUNE RESPONSE MANKIND PHENOLIC COMPOUNDS REPONSE IMMUNITAIRE RESPUESTA INMUNOLOGICA TEST ELISA |
| title | A surface plasmon resonance-based immunosensor for sensitive detection of bisphenol A |
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