A rapid extraction-TLC method for differentiation of Burkholderia spp., Ralstonia solanacearum, Herbaspirillum rubrisubalbicans and Pseudomonas syringae Pathovars

A rapid extraction-TLC method was conducted for differentiation of Burkholderia spp., Ralstonia solanacearum, Herbaspirillum rubrisubalbicans and Pseudomonas syringae pathovars. Lipids were extracted from bacterial cells in a small glass-vial using 0.2ml of chloroform - methanol - 0.3% Nacl solution...

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Veröffentlicht in:	Journal of the Faculty of Agriculture, Kyushu University Kyushu University, 1999-11, Vol.44 (1-2), p.49-58
Hauptverfasser:	Khan, A.A. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture), Matsuyama, N
Format:	Artikel
Sprache:	eng
Schlagworte:	BACTERIA CELLS CELLULE CELULAS CHROMATOGRAPHIE EN COUCHE MINCE CLASIFICACION CLASSIFICATION CROMATOGRAFIA DE CAPA FINA EXTRACCION EXTRACTION LIPIDE LIPIDOS LIPIDS METHODE METHODS METODOS PSEUDOMONAS THIN LAYER CHROMATOGRAPHY
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container_title	Journal of the Faculty of Agriculture, Kyushu University
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creator	Khan, A.A. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) Matsuyama, N
description	A rapid extraction-TLC method was conducted for differentiation of Burkholderia spp., Ralstonia solanacearum, Herbaspirillum rubrisubalbicans and Pseudomonas syringae pathovars. Lipids were extracted from bacterial cells in a small glass-vial using 0.2ml of chloroform - methanol - 0.3% Nacl solution (2:1: 0.4, v/v/v). The development of TLC plate was performed with chloroform - methanol - 0.2% CaCl2.H20 solution (55:35:8, v/v/v) for 1 h at 25 degrees C amino-lipid spots were visualized by spraying ninhydrin and successive heating at 100 degrees C for 10 min. The chromatograms were characteristic at both the generic and species level. This method will be practical for rapid differentiation as well as identification of Burkholderia spp., R. solanacearum, H. rubrisubalbicans and P. syringae pathovars
doi_str_mv	10.5109/24305
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subjects	BACTERIA CELLS CELLULE CELULAS CHROMATOGRAPHIE EN COUCHE MINCE CLASIFICACION CLASSIFICATION CROMATOGRAFIA DE CAPA FINA EXTRACCION EXTRACTION LIPIDE LIPIDOS LIPIDS METHODE METHODS METODOS PSEUDOMONAS THIN LAYER CHROMATOGRAPHY
title	A rapid extraction-TLC method for differentiation of Burkholderia spp., Ralstonia solanacearum, Herbaspirillum rubrisubalbicans and Pseudomonas syringae Pathovars
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