The Rapid and Precise Detection of Candida albicans in Cosmetic Products Using Loop-Mediated Isothermal Amplification (LAMP)
The loop-mediated isothermal amplification (LAMP) method can be expected to shorten the time required to detect specific micro-organisms in cosmetic products by the microbial limit test. However, the LAMP reaction failed to detect Candida albicans when test samples were contaminated with this specie...
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| Veröffentlicht in: | Journal of Society of Cosmetic Chemists of Japan 2018/03/20, Vol.52(1), pp.8-15 |
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| container_title | Journal of Society of Cosmetic Chemists of Japan |
| container_volume | 52 |
| creator | Ota, Seiko Niimura, Takako Azuma, Ryuta Suzuki, Ruka Kameyama, Koichi Nakade, Masato |
| description | The loop-mediated isothermal amplification (LAMP) method can be expected to shorten the time required to detect specific micro-organisms in cosmetic products by the microbial limit test. However, the LAMP reaction failed to detect Candida albicans when test samples were contaminated with this species and other bacteria. This was because the concentration of C. albicans (a kind of yeast) did not exceed the detection limit due to inhibition by the growth of other bacteria in pre-culture medium. To solve this problem, we improved the pre-culture conditions to promote the growth of C. albicans. We also found that the dilution step in the LAMP protocol could be omitted and the precise detection of C. albicans be achieved by the LAMP method for almost all cosmetic products. |
| doi_str_mv | 10.5107/sccj.52.8 |
| format | Article |
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We also found that the dilution step in the LAMP protocol could be omitted and the precise detection of C. albicans be achieved by the LAMP method for almost all cosmetic products.</description><subject>Candida albicans</subject><subject>cationic polymers</subject><subject>cosmetic products</subject><subject>detection</subject><subject>DNA</subject><subject>LAMP method</subject><subject>microbial limit test</subject><subject>precision</subject><subject>shipment</subject><subject>specified microorganisms</subject><issn>0387-5253</issn><issn>1884-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kEtrwzAQhEVpoSHNof9Ax-bgVLIkP6CX4L4CDg0lPRt5JSUKtmUk91Doj6-TlJyW3f1mYAahe0oWgpL0MQAcFiJeZFdoQrOMR5zy5BpNCMvSSMSC3aJZCLYmRNCcU0Em6He71_hT9lZh2Sm88Rps0PhZDxoG6zrsDC7Gj1USy6a2ILuAbYcLF1o9WBgVTn3DEPBXsN0Ol8710VorKwet8Cq4Ya99Kxu8bPvGmlF_cn0ol-vN_A7dGNkEPfufU7R9fdkW71H58bYqlmUEWdpFiWCCaaiVhBwywg2HWnCSEMJZbBiHnI7ZDOcqUTKpc16Pe2KUilnM0pqyKZqfbcG7ELw2Ve9tK_1PRUl1LK46FleJuMpG9unMHsIgd_pCSj-GbfSFpCf8coa99JXu2B-JlHfg</recordid><startdate>20180320</startdate><enddate>20180320</enddate><creator>Ota, Seiko</creator><creator>Niimura, Takako</creator><creator>Azuma, Ryuta</creator><creator>Suzuki, Ruka</creator><creator>Kameyama, Koichi</creator><creator>Nakade, Masato</creator><general>The Society of Cosmetic Chemists of Japan</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180320</creationdate><title>The Rapid and Precise Detection of Candida albicans in Cosmetic Products Using Loop-Mediated Isothermal Amplification (LAMP)</title><author>Ota, Seiko ; Niimura, Takako ; Azuma, Ryuta ; Suzuki, Ruka ; Kameyama, Koichi ; Nakade, Masato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c87n-65353ecbdac9c804f4cb540600432f34c91525f44d6da6b94b1526fdd23237b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2018</creationdate><topic>Candida albicans</topic><topic>cationic polymers</topic><topic>cosmetic products</topic><topic>detection</topic><topic>DNA</topic><topic>LAMP method</topic><topic>microbial limit test</topic><topic>precision</topic><topic>shipment</topic><topic>specified microorganisms</topic><toplevel>online_resources</toplevel><creatorcontrib>Ota, Seiko</creatorcontrib><creatorcontrib>Niimura, Takako</creatorcontrib><creatorcontrib>Azuma, Ryuta</creatorcontrib><creatorcontrib>Suzuki, Ruka</creatorcontrib><creatorcontrib>Kameyama, Koichi</creatorcontrib><creatorcontrib>Nakade, Masato</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Society of Cosmetic Chemists of Japan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ota, Seiko</au><au>Niimura, Takako</au><au>Azuma, Ryuta</au><au>Suzuki, Ruka</au><au>Kameyama, Koichi</au><au>Nakade, Masato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Rapid and Precise Detection of Candida albicans in Cosmetic Products Using Loop-Mediated Isothermal Amplification (LAMP)</atitle><jtitle>Journal of Society of Cosmetic Chemists of Japan</jtitle><addtitle>Journal of Society of Cosmetic Chemists of Japan</addtitle><date>2018-03-20</date><risdate>2018</risdate><volume>52</volume><issue>1</issue><spage>8</spage><epage>15</epage><pages>8-15</pages><issn>0387-5253</issn><eissn>1884-4146</eissn><abstract>The loop-mediated isothermal amplification (LAMP) method can be expected to shorten the time required to detect specific micro-organisms in cosmetic products by the microbial limit test. 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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | Candida albicans cationic polymers cosmetic products detection DNA LAMP method microbial limit test precision shipment specified microorganisms |
| title | The Rapid and Precise Detection of Candida albicans in Cosmetic Products Using Loop-Mediated Isothermal Amplification (LAMP) |
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