A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days

Background: Laurel wilt disease has caused the extensive mortality of lauraceous species in the southeastern United States. The causal agent is an invasive fungus, Raffaelea lauricola, which is a symbiont of the beetle Xyleborus glabratus and causes a rapid, fatal vascular wilt. Early diagnosis of l...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Arboriculture & urban forestry 2021-05, Vol.47 (3), p.98-109
Hauptverfasser: Hamilton, Jeffrey, Fraedrich, Stephen, Nairn, Campbell, Mayfield, Albert, Villari, Caterina
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 109
container_issue 3
container_start_page 98
container_title Arboriculture & urban forestry
container_volume 47
creator Hamilton, Jeffrey
Fraedrich, Stephen
Nairn, Campbell
Mayfield, Albert
Villari, Caterina
description Background: Laurel wilt disease has caused the extensive mortality of lauraceous species in the southeastern United States. The causal agent is an invasive fungus, Raffaelea lauricola, which is a symbiont of the beetle Xyleborus glabratus and causes a rapid, fatal vascular wilt. Early diagnosis of laurel wilt is imperative for efficient disease management. The current diagnostic process, however, is slow due to the lengthy laboratory procedures required to confirm pathogen presence. Methods: We tested the robustness and field-portability of a recently developed, species-specific, loop-mediated isothermal amplification (LAMP) assay for R. lauricola, with the overall goal of eliminating the need for a laboratory confirmation of the diagnosis. We tested the robustness of the assay using benchtop equipment with naturally infected samples. We then tested the assay directly in the field using a portable device. Results: The assay successfully detected R. lauricola directly from symptomatic wood tissue using crude DNA extracts. Furthermore, the assay readily allowed users to distinguish between symptoms caused by R. lauricola infection and similar symptoms caused by other agents. In-field, we assayed wood samples from symptomatic redbay (Persea borbonia [L.] Spreng) and sassafras (Sassafras albidum [Nutt.] Nees) across the Southeast and successfully detected R. lauricola-infected trees in less than an hour. Conclusion: Results of this study confirmed that the field-deployable LAMP assay is robust and can rapidly and accurately detect R. lauricola in infected trees directly on-site. LAMP technology is well suited for in-field implementation, and these results serve as an incentive for further development and use of this technology in the field of forest pathology.
doi_str_mv 10.48044/jauf.2021.010
format Article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_48044_jauf_2021_010</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_48044_jauf_2021_010</sourcerecordid><originalsourceid>FETCH-LOGICAL-c154t-e89a2be84fb71bb5f1ac9f4154ddd7694c0fc1160bc5e8b6d5539e09528ea46f3</originalsourceid><addsrcrecordid>eNo90DFPwzAQBWALgUQprMz-Awm2YyfxWLWUViqCAcQYnZ0zuEqTypcO_fe0gJhuePfe8DF2L0Wua6H1wxYOIVdCyVxIccEmShqTiaqqL9lE2sJkRtnqmt0QbYXQldLFhIUZX0bs2ux1SCO4Dvkiwmc_0Bg9n-33aQD_xedDH2LaEd_AIWHHP2I3nh4Jgf4LkXjs-XPsDyMSX_c0IrR8CHwBR7plVwE6wru_O2Xvy8e3-SrbvDyt57NN5qXRY4a1BeWw1sFV0jkTJHgb9Clr27YqrfYieClL4bzB2pWtMYVFYY2qEXQZiinLf3d9GogShmaf4g7SsZGi-VFqzkrNWak5KRXfvZpcZg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Hamilton, Jeffrey ; Fraedrich, Stephen ; Nairn, Campbell ; Mayfield, Albert ; Villari, Caterina</creator><creatorcontrib>Hamilton, Jeffrey ; Fraedrich, Stephen ; Nairn, Campbell ; Mayfield, Albert ; Villari, Caterina</creatorcontrib><description>Background: Laurel wilt disease has caused the extensive mortality of lauraceous species in the southeastern United States. The causal agent is an invasive fungus, Raffaelea lauricola, which is a symbiont of the beetle Xyleborus glabratus and causes a rapid, fatal vascular wilt. Early diagnosis of laurel wilt is imperative for efficient disease management. The current diagnostic process, however, is slow due to the lengthy laboratory procedures required to confirm pathogen presence. Methods: We tested the robustness and field-portability of a recently developed, species-specific, loop-mediated isothermal amplification (LAMP) assay for R. lauricola, with the overall goal of eliminating the need for a laboratory confirmation of the diagnosis. We tested the robustness of the assay using benchtop equipment with naturally infected samples. We then tested the assay directly in the field using a portable device. Results: The assay successfully detected R. lauricola directly from symptomatic wood tissue using crude DNA extracts. Furthermore, the assay readily allowed users to distinguish between symptoms caused by R. lauricola infection and similar symptoms caused by other agents. In-field, we assayed wood samples from symptomatic redbay (Persea borbonia [L.] Spreng) and sassafras (Sassafras albidum [Nutt.] Nees) across the Southeast and successfully detected R. lauricola-infected trees in less than an hour. Conclusion: Results of this study confirmed that the field-deployable LAMP assay is robust and can rapidly and accurately detect R. lauricola in infected trees directly on-site. LAMP technology is well suited for in-field implementation, and these results serve as an incentive for further development and use of this technology in the field of forest pathology.</description><identifier>ISSN: 1935-5297</identifier><identifier>EISSN: 2155-0778</identifier><identifier>DOI: 10.48044/jauf.2021.010</identifier><language>eng</language><ispartof>Arboriculture &amp; urban forestry, 2021-05, Vol.47 (3), p.98-109</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c154t-e89a2be84fb71bb5f1ac9f4154ddd7694c0fc1160bc5e8b6d5539e09528ea46f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Hamilton, Jeffrey</creatorcontrib><creatorcontrib>Fraedrich, Stephen</creatorcontrib><creatorcontrib>Nairn, Campbell</creatorcontrib><creatorcontrib>Mayfield, Albert</creatorcontrib><creatorcontrib>Villari, Caterina</creatorcontrib><title>A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days</title><title>Arboriculture &amp; urban forestry</title><description>Background: Laurel wilt disease has caused the extensive mortality of lauraceous species in the southeastern United States. The causal agent is an invasive fungus, Raffaelea lauricola, which is a symbiont of the beetle Xyleborus glabratus and causes a rapid, fatal vascular wilt. Early diagnosis of laurel wilt is imperative for efficient disease management. The current diagnostic process, however, is slow due to the lengthy laboratory procedures required to confirm pathogen presence. Methods: We tested the robustness and field-portability of a recently developed, species-specific, loop-mediated isothermal amplification (LAMP) assay for R. lauricola, with the overall goal of eliminating the need for a laboratory confirmation of the diagnosis. We tested the robustness of the assay using benchtop equipment with naturally infected samples. We then tested the assay directly in the field using a portable device. Results: The assay successfully detected R. lauricola directly from symptomatic wood tissue using crude DNA extracts. Furthermore, the assay readily allowed users to distinguish between symptoms caused by R. lauricola infection and similar symptoms caused by other agents. In-field, we assayed wood samples from symptomatic redbay (Persea borbonia [L.] Spreng) and sassafras (Sassafras albidum [Nutt.] Nees) across the Southeast and successfully detected R. lauricola-infected trees in less than an hour. Conclusion: Results of this study confirmed that the field-deployable LAMP assay is robust and can rapidly and accurately detect R. lauricola in infected trees directly on-site. LAMP technology is well suited for in-field implementation, and these results serve as an incentive for further development and use of this technology in the field of forest pathology.</description><issn>1935-5297</issn><issn>2155-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNo90DFPwzAQBWALgUQprMz-Awm2YyfxWLWUViqCAcQYnZ0zuEqTypcO_fe0gJhuePfe8DF2L0Wua6H1wxYOIVdCyVxIccEmShqTiaqqL9lE2sJkRtnqmt0QbYXQldLFhIUZX0bs2ux1SCO4Dvkiwmc_0Bg9n-33aQD_xedDH2LaEd_AIWHHP2I3nh4Jgf4LkXjs-XPsDyMSX_c0IrR8CHwBR7plVwE6wru_O2Xvy8e3-SrbvDyt57NN5qXRY4a1BeWw1sFV0jkTJHgb9Clr27YqrfYieClL4bzB2pWtMYVFYY2qEXQZiinLf3d9GogShmaf4g7SsZGi-VFqzkrNWak5KRXfvZpcZg</recordid><startdate>20210503</startdate><enddate>20210503</enddate><creator>Hamilton, Jeffrey</creator><creator>Fraedrich, Stephen</creator><creator>Nairn, Campbell</creator><creator>Mayfield, Albert</creator><creator>Villari, Caterina</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20210503</creationdate><title>A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days</title><author>Hamilton, Jeffrey ; Fraedrich, Stephen ; Nairn, Campbell ; Mayfield, Albert ; Villari, Caterina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c154t-e89a2be84fb71bb5f1ac9f4154ddd7694c0fc1160bc5e8b6d5539e09528ea46f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamilton, Jeffrey</creatorcontrib><creatorcontrib>Fraedrich, Stephen</creatorcontrib><creatorcontrib>Nairn, Campbell</creatorcontrib><creatorcontrib>Mayfield, Albert</creatorcontrib><creatorcontrib>Villari, Caterina</creatorcontrib><collection>CrossRef</collection><jtitle>Arboriculture &amp; urban forestry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamilton, Jeffrey</au><au>Fraedrich, Stephen</au><au>Nairn, Campbell</au><au>Mayfield, Albert</au><au>Villari, Caterina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days</atitle><jtitle>Arboriculture &amp; urban forestry</jtitle><date>2021-05-03</date><risdate>2021</risdate><volume>47</volume><issue>3</issue><spage>98</spage><epage>109</epage><pages>98-109</pages><issn>1935-5297</issn><eissn>2155-0778</eissn><abstract>Background: Laurel wilt disease has caused the extensive mortality of lauraceous species in the southeastern United States. The causal agent is an invasive fungus, Raffaelea lauricola, which is a symbiont of the beetle Xyleborus glabratus and causes a rapid, fatal vascular wilt. Early diagnosis of laurel wilt is imperative for efficient disease management. The current diagnostic process, however, is slow due to the lengthy laboratory procedures required to confirm pathogen presence. Methods: We tested the robustness and field-portability of a recently developed, species-specific, loop-mediated isothermal amplification (LAMP) assay for R. lauricola, with the overall goal of eliminating the need for a laboratory confirmation of the diagnosis. We tested the robustness of the assay using benchtop equipment with naturally infected samples. We then tested the assay directly in the field using a portable device. Results: The assay successfully detected R. lauricola directly from symptomatic wood tissue using crude DNA extracts. Furthermore, the assay readily allowed users to distinguish between symptoms caused by R. lauricola infection and similar symptoms caused by other agents. In-field, we assayed wood samples from symptomatic redbay (Persea borbonia [L.] Spreng) and sassafras (Sassafras albidum [Nutt.] Nees) across the Southeast and successfully detected R. lauricola-infected trees in less than an hour. Conclusion: Results of this study confirmed that the field-deployable LAMP assay is robust and can rapidly and accurately detect R. lauricola in infected trees directly on-site. LAMP technology is well suited for in-field implementation, and these results serve as an incentive for further development and use of this technology in the field of forest pathology.</abstract><doi>10.48044/jauf.2021.010</doi><tpages>12</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1935-5297
ispartof Arboriculture & urban forestry, 2021-05, Vol.47 (3), p.98-109
issn 1935-5297
2155-0778
language eng
recordid cdi_crossref_primary_10_48044_jauf_2021_010
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
title A Field-Portable Diagnostic Approach Confirms Laurel Wilt Disease Diagnosis in Minutes Instead of Days
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T17%3A15%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Field-Portable%20Diagnostic%20Approach%20Confirms%20Laurel%20Wilt%20Disease%20Diagnosis%20in%20Minutes%20Instead%20of%20Days&rft.jtitle=Arboriculture%20&%20urban%20forestry&rft.au=Hamilton,%20Jeffrey&rft.date=2021-05-03&rft.volume=47&rft.issue=3&rft.spage=98&rft.epage=109&rft.pages=98-109&rft.issn=1935-5297&rft.eissn=2155-0778&rft_id=info:doi/10.48044/jauf.2021.010&rft_dat=%3Ccrossref%3E10_48044_jauf_2021_010%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true