Isolation of madA homologs in Pilobolus crystallinus
Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is co...
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| Veröffentlicht in: | Mycoscience 2009, Vol.50(5), pp.400-406 |
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| creator | Kubo, Hiroyoshi |
| description | Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3. However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation |
| doi_str_mv | 10.47371/mycosci.MYC50400 |
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Three genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3. However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation</description><identifier>ISSN: 1340-3540</identifier><identifier>EISSN: 1618-2545</identifier><identifier>DOI: 10.47371/mycosci.MYC50400</identifier><language>eng</language><publisher>The Mycological Society of Japan</publisher><subject>Blue light ; Mucorales ; Photoreceptor ; Phototropism</subject><ispartof>Mycoscience, 2009, Vol.50(5), pp.400-406</ispartof><rights>2009, by The Mycological Society of Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Kubo, Hiroyoshi</creatorcontrib><title>Isolation of madA homologs in Pilobolus crystallinus</title><title>Mycoscience</title><description>Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3. However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation</description><subject>Blue light</subject><subject>Mucorales</subject><subject>Photoreceptor</subject><subject>Phototropism</subject><issn>1340-3540</issn><issn>1618-2545</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNo9j8tOwzAQRS0EEqXwAez8Ayl-O11WEYVKRbCABStr6jitKydGdrrI3xPoQyPN3MWc0RyEHimZCc01fWoHG7P1s7fvShJByBWaUEXLgkkhr8fMBSm4FOQW3eW8J4RqxdkEiVWOAXofOxwb3EK9wLvYxhC3GfsOf_gQNzEcMrZpyD2E4LtDvkc3DYTsHk5zir6Wz5_Va7F-f1lVi3VhmaaksK7hlikJTGk7lpjbui7ByZpS0IKrDRDBLACUei4UrXXJBWeM8ZGpheRTRI93bYo5J9eYn-RbSIOhxPxrm5O2OWuPzPLI7Md3t-5CQOq9De4CSGLkXzuDlwW7g2Rcx38BfbdmjQ</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Kubo, Hiroyoshi</creator><general>The Mycological Society of Japan</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2009</creationdate><title>Isolation of madA homologs in Pilobolus crystallinus</title><author>Kubo, Hiroyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2710-cef3c265a267c7c749cdd8ae5d11a7436ba042caaa879461d78343222365ad453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Blue light</topic><topic>Mucorales</topic><topic>Photoreceptor</topic><topic>Phototropism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kubo, Hiroyoshi</creatorcontrib><collection>CrossRef</collection><jtitle>Mycoscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kubo, Hiroyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of madA homologs in Pilobolus crystallinus</atitle><jtitle>Mycoscience</jtitle><date>2009</date><risdate>2009</risdate><volume>50</volume><issue>5</issue><spage>400</spage><epage>406</epage><pages>400-406</pages><artnum>MYC50400</artnum><issn>1340-3540</issn><eissn>1618-2545</eissn><abstract>Pilobolus crystallinus shows unique photoresponses at various growing stages. cDNAs for putative photoreceptors were cloned from this fungus. Three genes named pcmada1, pcmada2, and pcmada3 were identified from the PCR fragments, and amplified with degenerated primers for the LOV domain, which is conserved in many blue-light receptors. Deduced amino acid sequences for PCMADA1, PCMADA2, and PCMADA3 had one light-oxygen-voltage (LOV)-sensing and two PER-ARNT-SIM (PAS) domains. A zinc finger DNA-binding motif was conserved in the C-terminals of PCMADA1 and PCMADA3. However, PCMADA2 lacked the zinc finger motif. Expression of pcmada1 was suppressed by blue light whereas that of pcmada3 was promoted by blue-light irradiation</abstract><pub>The Mycological Society of Japan</pub><doi>10.47371/mycosci.MYC50400</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Mycoscience, 2009, Vol.50(5), pp.400-406 |
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| language | eng |
| recordid | cdi_crossref_primary_10_47371_mycosci_MYC50400 |
| source | J-STAGE Free |
| subjects | Blue light Mucorales Photoreceptor Phototropism |
| title | Isolation of madA homologs in Pilobolus crystallinus |
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