Penambahan Yolk Puyuh, Sari Pepaya (Carica papaya L.), Ringer’s serta Lama Penyimpanan pada Suhu 50C terhadap Kualitas Spermatozoa Ayam KUB: Addition of Quail Yolk, Papaya Juice (Carica papaya L.), Ringer's and Storage Time at 50C Quality of KUB Chicken Spermatozoa

Abstract KUB chickens have a low volume of semen per ejaculate, but the concentration is high. IB volume and concentration requirements must be met. Papaya (Carica papaya L.), is rich in carbohydrates, protein, fat, fiber, antioxidants and vitamins. Quail yolk contains lipoprotein, fat, carbohydrates, minerals and vitamin A. The addition of papaya juice in ringer's lactate diluent + quail yolk as a diluent for KUB chicken semen is thought to improve the quality of KUB chicken spermatozoa during storage. Experimental research, using a Completely Randomized Factorial Design with two factors; the first factor is diluent with two levels, namely level I (ringer's lactate + quail yolk) and II (ringer's lactate + quail yolk + papaya juice); while the second factor is the length of time of storage in the refrigerator at 50C consisting of five levels namely 0, 2, 4, 6 and 8 hours. The research variables included volume, consistency, pH, color and odor, concentration of spermatozoa, motility of spermatozoa (%), viability of spermatozoa and abnormality of spermatozoa. The results showed that there was a significant interaction between adding papaya juice to ringer's diluent + quail yolk and storage time at 50C on the quality of semen (spermatozoa) of KUB chickens. The addition of papaya juice to ringer's diluent + quail yolk had a good effect on maintaining motility, viability and abnormal spermatozoa of KUB chickens. The length of storage time has a significant effect on the quality of semen (spermatozoa) of KUB chickens. Keywords: Carica papaya; KUB chicken; Spermatozoa Abstrak Ayam KUB memiliki volume semen per ejakulat rendah, namun konsentrasinya tinggi. Syarat volume dan konsentrasi IB harus terpenuhi. Pepaya (Carica papaya L.), kaya kandungan karbohidrat, protein, lemak, serat, antioksidan, vitamin A, B1, B2, B3, B5, B6, asam folat, vitamin C, E dan K. Yolk puyuh mengandung lipoprotein, lemak, karbohidrat, mineral serta vitamin A. Penambahan sari buah pepaya dalam pengencer ringer’s laktat + yolk puyuh sebagai pengencer semen ayam KUB diduga dapat meningkatkan kualitas spermatozoa ayam KUB selama penyimpanan. Penelitian secara eksperimen, menggunakan Rancangan Acak Lengkap Faktorial (RAL-Faktorial) dengan 2 (dua) faktor; faktor pertama adalah bahan pengencer dengan 2 (dua) level yaitu level I (ringer’s laktat + yolk puyuh) dan II (ringer’s laktat + yolk puyuh + sari buah pepaya); sedangkan faktor kedua adalah lama waktu penyimpanan didalam refrigerator pad