New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets
Purpose: To develop a new spectrophotometric method with improved sensitivity and at higher wavelength for the determination of p-aminosalicylic acid in tablets. Methods: Two simple and sensitive spectrophotometric methods (methods A and B) were developed using p-dimethylaminobenzaldehyde (DAB), and...
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| Veröffentlicht in: | Tropical journal of pharmaceutical research 2014-09, Vol.13 (7), p.1133 |
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| container_title | Tropical journal of pharmaceutical research |
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| creator | Laghari, M.G.H Darwis, Y Memon, A.H |
| description | Purpose: To develop a new spectrophotometric method with improved
sensitivity and at higher wavelength for the determination of
p-aminosalicylic acid in tablets. Methods: Two simple and sensitive
spectrophotometric methods (methods A and B) were developed using
p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde
(DAC) as derivatizing reagents for the determination of
p-aminosalicylic acid (PAS) in tablets. The derivatization was carried
out using 3M HCl-KCl buffer for DAB and 5M HCl-KCl buffer solutions.
Result: The new derivatives of PAS absorbed maximally with bathochromic
shift to 460 and 555 nm in the linear concentration range of 0.4
- 2.0 μg/mL with molar absorptivities of 2.4 × 104 and
3.8 × 104 L/mole/cm, respectively, compared to pure PAS which
absorbed at λmax of 264 nm with molar absorptivity 7.65 × 103
L/mole/cm in the linear concentration range of 2 - 10 μg/mL. The
developed methods were successfully applied to assay PAS in tablets
with % recovery of 97.6 ± 1.71 and 98.4 ± 1.45 for methods A
and B, respectively. Conclusion: Both PAS derivatives absorb in the
visible spectral region. The presence of excipients in pharmaceutical
preparations did not interfere in the determination of PAS as PAS-DAB
and PAS-DAC derivatives. Both methods can be applied to determine PAS
from bulk and various pharmaceutical dosage forms. |
| doi_str_mv | 10.4314/tjpr.v13i7.18 |
| format | Article |
| fullrecord | <record><control><sourceid>bioline_cross</sourceid><recordid>TN_cdi_crossref_primary_10_4314_tjpr_v13i7_18</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_pr_pr14158</sourcerecordid><originalsourceid>FETCH-LOGICAL-b276t-c4547d538507788d03e06cc6026590ebeb50200d7bef11a892ad94c5c59d60243</originalsourceid><addsrcrecordid>eNpFkE1LAzEQhoMoWKtH7_kDuya7ySY5lvoJVQ9W8BayySxN2W6WJCj9926tKMww7wwPc3gQuqakZDVlN3k7xvKT1l6UVJ6gGeWqKZSsxOlv5ko15-gipS0hvFGKztDHC3zhtxFsjmHchBx2kKO3-BnyJriEuxBx3gC-hQxx5weTfRhw6PBYLKY1JNN7u58aL6x32A94bdoecrpEZ53pE1z9zjl6v79bLx-L1evD03KxKtpKNLmwjDPheC05EUJKR2ogjbUNqRquCLTQclIR4kQLHaVGqso4xSy3XLkJYvUcFce_NoaUInR6jH5n4l5Tog9a9EGL_tGiqZz48si3PvR-gD_cRm_0_3EqyiiX9TcvsGj0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>African Journals Online (Open Access)</source><source>Bioline International</source><source>Free Full-Text Journals in Chemistry</source><creator>Laghari, M.G.H ; Darwis, Y ; Memon, A.H</creator><creatorcontrib>Laghari, M.G.H ; Darwis, Y ; Memon, A.H</creatorcontrib><description>Purpose: To develop a new spectrophotometric method with improved
sensitivity and at higher wavelength for the determination of
p-aminosalicylic acid in tablets. Methods: Two simple and sensitive
spectrophotometric methods (methods A and B) were developed using
p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde
(DAC) as derivatizing reagents for the determination of
p-aminosalicylic acid (PAS) in tablets. The derivatization was carried
out using 3M HCl-KCl buffer for DAB and 5M HCl-KCl buffer solutions.
Result: The new derivatives of PAS absorbed maximally with bathochromic
shift to 460 and 555 nm in the linear concentration range of 0.4
- 2.0 μg/mL with molar absorptivities of 2.4 × 104 and
3.8 × 104 L/mole/cm, respectively, compared to pure PAS which
absorbed at λmax of 264 nm with molar absorptivity 7.65 × 103
L/mole/cm in the linear concentration range of 2 - 10 μg/mL. The
developed methods were successfully applied to assay PAS in tablets
with % recovery of 97.6 ± 1.71 and 98.4 ± 1.45 for methods A
and B, respectively. Conclusion: Both PAS derivatives absorb in the
visible spectral region. The presence of excipients in pharmaceutical
preparations did not interfere in the determination of PAS as PAS-DAB
and PAS-DAC derivatives. Both methods can be applied to determine PAS
from bulk and various pharmaceutical dosage forms.</description><identifier>ISSN: 1596-5996</identifier><identifier>EISSN: 1596-9827</identifier><identifier>DOI: 10.4314/tjpr.v13i7.18</identifier><language>eng</language><publisher>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</publisher><subject>p-Aminosalicylic acid ; p-dimethylaminobenzaldehyde ; p-dimethylaminocinnamaldehyde ; Spectrophotometry</subject><ispartof>Tropical journal of pharmaceutical research, 2014-09, Vol.13 (7), p.1133</ispartof><rights>Copyright 2014 - Tropical Journal of Pharmaceutical Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b276t-c4547d538507788d03e06cc6026590ebeb50200d7bef11a892ad94c5c59d60243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925,79426</link.rule.ids></links><search><creatorcontrib>Laghari, M.G.H</creatorcontrib><creatorcontrib>Darwis, Y</creatorcontrib><creatorcontrib>Memon, A.H</creatorcontrib><title>New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets</title><title>Tropical journal of pharmaceutical research</title><description>Purpose: To develop a new spectrophotometric method with improved
sensitivity and at higher wavelength for the determination of
p-aminosalicylic acid in tablets. Methods: Two simple and sensitive
spectrophotometric methods (methods A and B) were developed using
p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde
(DAC) as derivatizing reagents for the determination of
p-aminosalicylic acid (PAS) in tablets. The derivatization was carried
out using 3M HCl-KCl buffer for DAB and 5M HCl-KCl buffer solutions.
Result: The new derivatives of PAS absorbed maximally with bathochromic
shift to 460 and 555 nm in the linear concentration range of 0.4
- 2.0 μg/mL with molar absorptivities of 2.4 × 104 and
3.8 × 104 L/mole/cm, respectively, compared to pure PAS which
absorbed at λmax of 264 nm with molar absorptivity 7.65 × 103
L/mole/cm in the linear concentration range of 2 - 10 μg/mL. The
developed methods were successfully applied to assay PAS in tablets
with % recovery of 97.6 ± 1.71 and 98.4 ± 1.45 for methods A
and B, respectively. Conclusion: Both PAS derivatives absorb in the
visible spectral region. The presence of excipients in pharmaceutical
preparations did not interfere in the determination of PAS as PAS-DAB
and PAS-DAC derivatives. Both methods can be applied to determine PAS
from bulk and various pharmaceutical dosage forms.</description><subject>p-Aminosalicylic acid</subject><subject>p-dimethylaminobenzaldehyde</subject><subject>p-dimethylaminocinnamaldehyde</subject><subject>Spectrophotometry</subject><issn>1596-5996</issn><issn>1596-9827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNpFkE1LAzEQhoMoWKtH7_kDuya7ySY5lvoJVQ9W8BayySxN2W6WJCj9926tKMww7wwPc3gQuqakZDVlN3k7xvKT1l6UVJ6gGeWqKZSsxOlv5ko15-gipS0hvFGKztDHC3zhtxFsjmHchBx2kKO3-BnyJriEuxBx3gC-hQxx5weTfRhw6PBYLKY1JNN7u58aL6x32A94bdoecrpEZ53pE1z9zjl6v79bLx-L1evD03KxKtpKNLmwjDPheC05EUJKR2ogjbUNqRquCLTQclIR4kQLHaVGqso4xSy3XLkJYvUcFce_NoaUInR6jH5n4l5Tog9a9EGL_tGiqZz48si3PvR-gD_cRm_0_3EqyiiX9TcvsGj0</recordid><startdate>20140918</startdate><enddate>20140918</enddate><creator>Laghari, M.G.H</creator><creator>Darwis, Y</creator><creator>Memon, A.H</creator><general>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</general><scope>RBI</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140918</creationdate><title>New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets</title><author>Laghari, M.G.H ; Darwis, Y ; Memon, A.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b276t-c4547d538507788d03e06cc6026590ebeb50200d7bef11a892ad94c5c59d60243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>p-Aminosalicylic acid</topic><topic>p-dimethylaminobenzaldehyde</topic><topic>p-dimethylaminocinnamaldehyde</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laghari, M.G.H</creatorcontrib><creatorcontrib>Darwis, Y</creatorcontrib><creatorcontrib>Memon, A.H</creatorcontrib><collection>Bioline International</collection><collection>CrossRef</collection><jtitle>Tropical journal of pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laghari, M.G.H</au><au>Darwis, Y</au><au>Memon, A.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets</atitle><jtitle>Tropical journal of pharmaceutical research</jtitle><date>2014-09-18</date><risdate>2014</risdate><volume>13</volume><issue>7</issue><spage>1133</spage><pages>1133-</pages><issn>1596-5996</issn><eissn>1596-9827</eissn><abstract>Purpose: To develop a new spectrophotometric method with improved
sensitivity and at higher wavelength for the determination of
p-aminosalicylic acid in tablets. Methods: Two simple and sensitive
spectrophotometric methods (methods A and B) were developed using
p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde
(DAC) as derivatizing reagents for the determination of
p-aminosalicylic acid (PAS) in tablets. The derivatization was carried
out using 3M HCl-KCl buffer for DAB and 5M HCl-KCl buffer solutions.
Result: The new derivatives of PAS absorbed maximally with bathochromic
shift to 460 and 555 nm in the linear concentration range of 0.4
- 2.0 μg/mL with molar absorptivities of 2.4 × 104 and
3.8 × 104 L/mole/cm, respectively, compared to pure PAS which
absorbed at λmax of 264 nm with molar absorptivity 7.65 × 103
L/mole/cm in the linear concentration range of 2 - 10 μg/mL. The
developed methods were successfully applied to assay PAS in tablets
with % recovery of 97.6 ± 1.71 and 98.4 ± 1.45 for methods A
and B, respectively. Conclusion: Both PAS derivatives absorb in the
visible spectral region. The presence of excipients in pharmaceutical
preparations did not interfere in the determination of PAS as PAS-DAB
and PAS-DAC derivatives. Both methods can be applied to determine PAS
from bulk and various pharmaceutical dosage forms.</abstract><pub>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</pub><doi>10.4314/tjpr.v13i7.18</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1596-5996 |
| ispartof | Tropical journal of pharmaceutical research, 2014-09, Vol.13 (7), p.1133 |
| issn | 1596-5996 1596-9827 |
| language | eng |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; African Journals Online (Open Access); Bioline International; Free Full-Text Journals in Chemistry |
| subjects | p-Aminosalicylic acid p-dimethylaminobenzaldehyde p-dimethylaminocinnamaldehyde Spectrophotometry |
| title | New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets |
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