High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma
Purpose: To identify and quantify anethole in the essential oil of fruits of Illicium verum Hook (star anise) and in vivo in rat plasma using reverse-phase liquid chromatography. Methods: Anethole was identified in the essential oil of the fruits of Star anise and determined by gas chromatography-ta...
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| Veröffentlicht in: | Tropical journal of pharmaceutical research 2014-09, Vol.13 (5), p.793 |
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| container_title | Tropical journal of pharmaceutical research |
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| creator | Fagundes, Vinicius HV Pinho, Rilson J Wiirzler, Luiz AM Kimura, Elza Bersani-Amado, Ciomar A Cuman, Roberto KN |
| description | Purpose: To identify and quantify anethole in the essential oil of
fruits of Illicium verum Hook (star anise) and in vivo in rat plasma
using reverse-phase liquid chromatography. Methods: Anethole was
identified in the essential oil of the fruits of Star anise and
determined by gas chromatography-tandem mass spectrometry (GC-MS),
nuclear magnetic resonance (NMR), ultraviolet visible spectrophotometry
(UV-VIS). A simple, sensitive and validated high performance liguid
chromatography (HPLC) technique with UV-VIS detection method was
developed for the determination of the compound in rat plasma using:
methanol-water (85:15, v/v) as mobile phase at a flow rate of 0.2
ml/min Hypersil ODS Thermo (150 mm × 2.1 mm × 3.0 μM) as
column with wavelength detection at 259 nm. Results: GC determination
showed that anethole in the essential oil of star anise exhibited a
retention time of 21.02 min. The validation results for anethole in
plasma were satisfactory, with coefficient of determination (R2) of
0.9945 and relative standard deviation of < 3 %. HPLC run time of 4
min with a retention time of 2.73 min was the faster method to
determine anethole when compared to a previously reported method which
had a run time of 15 min. Conclusion: Anethole in the essential oil of
Illicium verum Hook can be identified and determined by GC-MS, NMR and
UV-VIS, and a superior HPLC method has been developed for the
determination of the compound in rat plasma. |
| doi_str_mv | 10.4314/tjpr.v13i5.21 |
| format | Article |
| fullrecord | <record><control><sourceid>bioline_cross</sourceid><recordid>TN_cdi_crossref_primary_10_4314_tjpr_v13i5_21</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_pr_pr14113</sourcerecordid><originalsourceid>FETCH-LOGICAL-b315t-efbc4806fdba187a3200f604502ec479c213e4880022e7bebb7ab9e200da9d333</originalsourceid><addsrcrecordid>eNpFkEtLAzEUhYMoWKtL9_kDM-Y1jyxLfVSoWESXEpKZm07KzKRmotB_36ktCgfu4fJxFh9Ct5SkglNxFzfbkP5Q7rKU0TM0oZnME1my4vzUMynzS3Q1DBtCslxKOkGfC7du8AqC9aHTfQV46b6-XY3nTfCdjn4d9LbZ4ReIja_xSOHYAL6HCKFzvY7O99hbPOsPQAvY9fhNR7xq9dDpa3RhdTvAzelO0cfjw_t8kSxfn57ns2ViOM1iAtZUoiS5rY2mZaE5I8TmRGSEQSUKWTHKQZQlIYxBYcCYQhsJI1VrWXPOpyg57lbBD0MAq7bBdTrsFCXq4EYd3KhfN2ocm6L0yBvnW9fDH14Fp9X_cwwVlHK-B36Maw8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma</title><source>DOAJ Directory of Open Access Journals</source><source>African Journals Online (Open Access)</source><source>Bioline International</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Fagundes, Vinicius HV ; Pinho, Rilson J ; Wiirzler, Luiz AM ; Kimura, Elza ; Bersani-Amado, Ciomar A ; Cuman, Roberto KN</creator><creatorcontrib>Fagundes, Vinicius HV ; Pinho, Rilson J ; Wiirzler, Luiz AM ; Kimura, Elza ; Bersani-Amado, Ciomar A ; Cuman, Roberto KN</creatorcontrib><description>Purpose: To identify and quantify anethole in the essential oil of
fruits of Illicium verum Hook (star anise) and in vivo in rat plasma
using reverse-phase liquid chromatography. Methods: Anethole was
identified in the essential oil of the fruits of Star anise and
determined by gas chromatography-tandem mass spectrometry (GC-MS),
nuclear magnetic resonance (NMR), ultraviolet visible spectrophotometry
(UV-VIS). A simple, sensitive and validated high performance liguid
chromatography (HPLC) technique with UV-VIS detection method was
developed for the determination of the compound in rat plasma using:
methanol-water (85:15, v/v) as mobile phase at a flow rate of 0.2
ml/min Hypersil ODS Thermo (150 mm × 2.1 mm × 3.0 μM) as
column with wavelength detection at 259 nm. Results: GC determination
showed that anethole in the essential oil of star anise exhibited a
retention time of 21.02 min. The validation results for anethole in
plasma were satisfactory, with coefficient of determination (R2) of
0.9945 and relative standard deviation of < 3 %. HPLC run time of 4
min with a retention time of 2.73 min was the faster method to
determine anethole when compared to a previously reported method which
had a run time of 15 min. Conclusion: Anethole in the essential oil of
Illicium verum Hook can be identified and determined by GC-MS, NMR and
UV-VIS, and a superior HPLC method has been developed for the
determination of the compound in rat plasma.</description><identifier>ISSN: 1596-5996</identifier><identifier>EISSN: 1596-9827</identifier><identifier>DOI: 10.4314/tjpr.v13i5.21</identifier><language>eng</language><publisher>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</publisher><subject>Anethole ; Essential oil ; High performance liguid chromatography ; Illicium verum Hook ; Rat plasma ; Star anise</subject><ispartof>Tropical journal of pharmaceutical research, 2014-09, Vol.13 (5), p.793</ispartof><rights>Copyright 2014 - Tropical Journal of Pharmaceutical Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b315t-efbc4806fdba187a3200f604502ec479c213e4880022e7bebb7ab9e200da9d333</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925,79426</link.rule.ids></links><search><creatorcontrib>Fagundes, Vinicius HV</creatorcontrib><creatorcontrib>Pinho, Rilson J</creatorcontrib><creatorcontrib>Wiirzler, Luiz AM</creatorcontrib><creatorcontrib>Kimura, Elza</creatorcontrib><creatorcontrib>Bersani-Amado, Ciomar A</creatorcontrib><creatorcontrib>Cuman, Roberto KN</creatorcontrib><title>High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma</title><title>Tropical journal of pharmaceutical research</title><description>Purpose: To identify and quantify anethole in the essential oil of
fruits of Illicium verum Hook (star anise) and in vivo in rat plasma
using reverse-phase liquid chromatography. Methods: Anethole was
identified in the essential oil of the fruits of Star anise and
determined by gas chromatography-tandem mass spectrometry (GC-MS),
nuclear magnetic resonance (NMR), ultraviolet visible spectrophotometry
(UV-VIS). A simple, sensitive and validated high performance liguid
chromatography (HPLC) technique with UV-VIS detection method was
developed for the determination of the compound in rat plasma using:
methanol-water (85:15, v/v) as mobile phase at a flow rate of 0.2
ml/min Hypersil ODS Thermo (150 mm × 2.1 mm × 3.0 μM) as
column with wavelength detection at 259 nm. Results: GC determination
showed that anethole in the essential oil of star anise exhibited a
retention time of 21.02 min. The validation results for anethole in
plasma were satisfactory, with coefficient of determination (R2) of
0.9945 and relative standard deviation of < 3 %. HPLC run time of 4
min with a retention time of 2.73 min was the faster method to
determine anethole when compared to a previously reported method which
had a run time of 15 min. Conclusion: Anethole in the essential oil of
Illicium verum Hook can be identified and determined by GC-MS, NMR and
UV-VIS, and a superior HPLC method has been developed for the
determination of the compound in rat plasma.</description><subject>Anethole</subject><subject>Essential oil</subject><subject>High performance liguid chromatography</subject><subject>Illicium verum Hook</subject><subject>Rat plasma</subject><subject>Star anise</subject><issn>1596-5996</issn><issn>1596-9827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNpFkEtLAzEUhYMoWKtL9_kDM-Y1jyxLfVSoWESXEpKZm07KzKRmotB_36ktCgfu4fJxFh9Ct5SkglNxFzfbkP5Q7rKU0TM0oZnME1my4vzUMynzS3Q1DBtCslxKOkGfC7du8AqC9aHTfQV46b6-XY3nTfCdjn4d9LbZ4ReIja_xSOHYAL6HCKFzvY7O99hbPOsPQAvY9fhNR7xq9dDpa3RhdTvAzelO0cfjw_t8kSxfn57ns2ViOM1iAtZUoiS5rY2mZaE5I8TmRGSEQSUKWTHKQZQlIYxBYcCYQhsJI1VrWXPOpyg57lbBD0MAq7bBdTrsFCXq4EYd3KhfN2ocm6L0yBvnW9fDH14Fp9X_cwwVlHK-B36Maw8</recordid><startdate>20140911</startdate><enddate>20140911</enddate><creator>Fagundes, Vinicius HV</creator><creator>Pinho, Rilson J</creator><creator>Wiirzler, Luiz AM</creator><creator>Kimura, Elza</creator><creator>Bersani-Amado, Ciomar A</creator><creator>Cuman, Roberto KN</creator><general>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</general><scope>RBI</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140911</creationdate><title>High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma</title><author>Fagundes, Vinicius HV ; Pinho, Rilson J ; Wiirzler, Luiz AM ; Kimura, Elza ; Bersani-Amado, Ciomar A ; Cuman, Roberto KN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b315t-efbc4806fdba187a3200f604502ec479c213e4880022e7bebb7ab9e200da9d333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anethole</topic><topic>Essential oil</topic><topic>High performance liguid chromatography</topic><topic>Illicium verum Hook</topic><topic>Rat plasma</topic><topic>Star anise</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fagundes, Vinicius HV</creatorcontrib><creatorcontrib>Pinho, Rilson J</creatorcontrib><creatorcontrib>Wiirzler, Luiz AM</creatorcontrib><creatorcontrib>Kimura, Elza</creatorcontrib><creatorcontrib>Bersani-Amado, Ciomar A</creatorcontrib><creatorcontrib>Cuman, Roberto KN</creatorcontrib><collection>Bioline International</collection><collection>CrossRef</collection><jtitle>Tropical journal of pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fagundes, Vinicius HV</au><au>Pinho, Rilson J</au><au>Wiirzler, Luiz AM</au><au>Kimura, Elza</au><au>Bersani-Amado, Ciomar A</au><au>Cuman, Roberto KN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma</atitle><jtitle>Tropical journal of pharmaceutical research</jtitle><date>2014-09-11</date><risdate>2014</risdate><volume>13</volume><issue>5</issue><spage>793</spage><pages>793-</pages><issn>1596-5996</issn><eissn>1596-9827</eissn><abstract>Purpose: To identify and quantify anethole in the essential oil of
fruits of Illicium verum Hook (star anise) and in vivo in rat plasma
using reverse-phase liquid chromatography. Methods: Anethole was
identified in the essential oil of the fruits of Star anise and
determined by gas chromatography-tandem mass spectrometry (GC-MS),
nuclear magnetic resonance (NMR), ultraviolet visible spectrophotometry
(UV-VIS). A simple, sensitive and validated high performance liguid
chromatography (HPLC) technique with UV-VIS detection method was
developed for the determination of the compound in rat plasma using:
methanol-water (85:15, v/v) as mobile phase at a flow rate of 0.2
ml/min Hypersil ODS Thermo (150 mm × 2.1 mm × 3.0 μM) as
column with wavelength detection at 259 nm. Results: GC determination
showed that anethole in the essential oil of star anise exhibited a
retention time of 21.02 min. The validation results for anethole in
plasma were satisfactory, with coefficient of determination (R2) of
0.9945 and relative standard deviation of < 3 %. HPLC run time of 4
min with a retention time of 2.73 min was the faster method to
determine anethole when compared to a previously reported method which
had a run time of 15 min. Conclusion: Anethole in the essential oil of
Illicium verum Hook can be identified and determined by GC-MS, NMR and
UV-VIS, and a superior HPLC method has been developed for the
determination of the compound in rat plasma.</abstract><pub>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</pub><doi>10.4314/tjpr.v13i5.21</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Tropical journal of pharmaceutical research, 2014-09, Vol.13 (5), p.793 |
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| source | DOAJ Directory of Open Access Journals; African Journals Online (Open Access); Bioline International; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Anethole Essential oil High performance liguid chromatography Illicium verum Hook Rat plasma Star anise |
| title | High Performance Liquid Chromatography Method for the Determination of Anethole in Rat Plasma |
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