Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis
Purpose: To investigate the immunoregulative effects of ulinastatin (UTI) on T lymphocytes apoptosis in rats with severe acute pancreatitis (SAP) and to elucidate its underlying molecular mechanism. Methods: Thirty six Wistar rats were randomly divided into 3 groups (n =12): sham, SAP model and UTI-...
Gespeichert in:
| Veröffentlicht in: | Tropical journal of pharmaceutical research 2014-01, Vol.13 (1), p.47 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 47 |
| container_title | Tropical journal of pharmaceutical research |
| container_volume | 13 |
| creator | Wang, Chunli Ma, Tao Zhou, Xiaolei Li, Nan You, Shengyi |
| description | Purpose: To investigate the immunoregulative effects of ulinastatin
(UTI) on T lymphocytes apoptosis in rats with severe acute pancreatitis
(SAP) and to elucidate its underlying molecular mechanism. Methods:
Thirty six Wistar rats were randomly divided into 3 groups (n =12):
sham, SAP model and UTI-treated group. SAP model was established by
intrapancreatobiliary duct injection of 5% sodium taurocholate. A bolus
of 10000 U/kg UTI was intravenously injected after SAP establishment. T
cell apoptosis was determined by Annexin-V/PI double-staining.
Oxidative stress was evaluated by examining changes in the levels of
reactive oxygen species (ROS). Total superoxide dismutase (SOD) in
serum was tested by hydroxylamine colorimetric assay, and
malondialdehyde levels were examined by thiobarbituric acid assay.
Mitochondrial function was evaluated by analyzing mitochondrial
membrane potential (MMP) and mitochondrial permeability transition pore
(MPTP). Results: We found CD4+ T cells (32.10±2.87% vs.
45.22±4.38%, P |
| doi_str_mv | 10.4314/tjpr.v13i1.7 |
| format | Article |
| fullrecord | <record><control><sourceid>bioline_cross</sourceid><recordid>TN_cdi_crossref_primary_10_4314_tjpr_v13i1_7</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_pr_pr14007</sourcerecordid><originalsourceid>FETCH-LOGICAL-b274t-5694c5da089739ecb9f9c43353658d1014bf5c3f61f1ae8fa88d507745deb513</originalsourceid><addsrcrecordid>eNpFkN1KAzEQhYMoWKt3PkAewF0zTbLZXJbiHxYUrdchm51gytpdkrTi27u1ojAww8w3Z5hDyCWwUnAQ13k9xHIHPECpjsgEpK4KXc_U8W8tta5OyVlKa8ZkpTVMyONbFzY2ZZvDhr5gu3WY6IousOvofOiH3KeQ6H5mc6KfIb_TV9xhRDp324z02W5cxHE7h3ROTrztEl785ilZ3d6sFvfF8unuYTFfFs1MiVyMl4WTrWW1Vlyja7TXTnAueSXrFhiIxkvHfQUeLNbe1nUrmVJCtthI4FNydZB1sU8pojdDDB82fhlgZu-D2ftgfnwwasTLA96EfvwV_2gXgzX_zTFAMKb4N-YUY3E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>African Journals Online (Open Access)</source><source>Bioline International</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Chunli ; Ma, Tao ; Zhou, Xiaolei ; Li, Nan ; You, Shengyi</creator><creatorcontrib>Wang, Chunli ; Ma, Tao ; Zhou, Xiaolei ; Li, Nan ; You, Shengyi</creatorcontrib><description><![CDATA[Purpose: To investigate the immunoregulative effects of ulinastatin
(UTI) on T lymphocytes apoptosis in rats with severe acute pancreatitis
(SAP) and to elucidate its underlying molecular mechanism. Methods:
Thirty six Wistar rats were randomly divided into 3 groups (n =12):
sham, SAP model and UTI-treated group. SAP model was established by
intrapancreatobiliary duct injection of 5% sodium taurocholate. A bolus
of 10000 U/kg UTI was intravenously injected after SAP establishment. T
cell apoptosis was determined by Annexin-V/PI double-staining.
Oxidative stress was evaluated by examining changes in the levels of
reactive oxygen species (ROS). Total superoxide dismutase (SOD) in
serum was tested by hydroxylamine colorimetric assay, and
malondialdehyde levels were examined by thiobarbituric acid assay.
Mitochondrial function was evaluated by analyzing mitochondrial
membrane potential (MMP) and mitochondrial permeability transition pore
(MPTP). Results: We found CD4+ T cells (32.10±2.87% vs.
45.22±4.38%, P<0.01) and CD4+/CD8+ T cells in SAP rats
significantly decreased compared with sham group (1.15±0.12 vs.
2.23±0.12%, P<0.01), while the percent of the apoptotic CD4+
and (17.70±2.10 vs. 3.82±0.50%, P<0.01) CD8+ T lymphocytes
was highly increased (2.78±0.45 vs. 1.97±0.36%, P<0.01
compared with sham group). After UTI treatment, the apoptosis of CD4+ T
lymphocytes significantly decreased compared with SAP group
(8.58±1.09 vs. 17.70±2.10%, P<0.01), while the percent of
CD4+ T and CD4+/CD8+ lymphocytes significantly enhanced (P<0.01).
ROS (mean fluorescence intensity): 5107±430 vs. 12904±840,
P<0.01) and MDA levels (4.41±0.32 vs. 7.25±0.57nmol/ml,
P<0.01) in serum in UTI-treated group were decreased compared with
SAP group. SOD activity was enhanced after UTI treatment
(59.72±5.45 vs. 48.32±3.81nmol/ml, P<0.01). Mitochondrial
function assays showed that MMP (17.30±1.60 vs. 46.94±3.49%,
P<0.01) and MPT (30.14±2.46 vs. 51.31±3.23%, P<0.01)
were inhibited by UTI. Conclusion: UTI reduces T lymphocytes apoptosis
and improves immunological function in SAP rats, possibly via enhancing
the scavenging capacity of oxygen free radical and attenuating the
influence of oxidative stress.]]></description><identifier>ISSN: 1596-5996</identifier><identifier>EISSN: 1596-9827</identifier><identifier>DOI: 10.4314/tjpr.v13i1.7</identifier><language>eng</language><publisher>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</publisher><subject>Apoptosis ; Mitochondrion ; Severe acute pancreatitis ; T cell ; Ulinastatin</subject><ispartof>Tropical journal of pharmaceutical research, 2014-01, Vol.13 (1), p.47</ispartof><rights>Copyright 2014 - Tropical Journal of Pharmaceutical Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b274t-5694c5da089739ecb9f9c43353658d1014bf5c3f61f1ae8fa88d507745deb513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906,79175</link.rule.ids></links><search><creatorcontrib>Wang, Chunli</creatorcontrib><creatorcontrib>Ma, Tao</creatorcontrib><creatorcontrib>Zhou, Xiaolei</creatorcontrib><creatorcontrib>Li, Nan</creatorcontrib><creatorcontrib>You, Shengyi</creatorcontrib><title>Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis</title><title>Tropical journal of pharmaceutical research</title><description><![CDATA[Purpose: To investigate the immunoregulative effects of ulinastatin
(UTI) on T lymphocytes apoptosis in rats with severe acute pancreatitis
(SAP) and to elucidate its underlying molecular mechanism. Methods:
Thirty six Wistar rats were randomly divided into 3 groups (n =12):
sham, SAP model and UTI-treated group. SAP model was established by
intrapancreatobiliary duct injection of 5% sodium taurocholate. A bolus
of 10000 U/kg UTI was intravenously injected after SAP establishment. T
cell apoptosis was determined by Annexin-V/PI double-staining.
Oxidative stress was evaluated by examining changes in the levels of
reactive oxygen species (ROS). Total superoxide dismutase (SOD) in
serum was tested by hydroxylamine colorimetric assay, and
malondialdehyde levels were examined by thiobarbituric acid assay.
Mitochondrial function was evaluated by analyzing mitochondrial
membrane potential (MMP) and mitochondrial permeability transition pore
(MPTP). Results: We found CD4+ T cells (32.10±2.87% vs.
45.22±4.38%, P<0.01) and CD4+/CD8+ T cells in SAP rats
significantly decreased compared with sham group (1.15±0.12 vs.
2.23±0.12%, P<0.01), while the percent of the apoptotic CD4+
and (17.70±2.10 vs. 3.82±0.50%, P<0.01) CD8+ T lymphocytes
was highly increased (2.78±0.45 vs. 1.97±0.36%, P<0.01
compared with sham group). After UTI treatment, the apoptosis of CD4+ T
lymphocytes significantly decreased compared with SAP group
(8.58±1.09 vs. 17.70±2.10%, P<0.01), while the percent of
CD4+ T and CD4+/CD8+ lymphocytes significantly enhanced (P<0.01).
ROS (mean fluorescence intensity): 5107±430 vs. 12904±840,
P<0.01) and MDA levels (4.41±0.32 vs. 7.25±0.57nmol/ml,
P<0.01) in serum in UTI-treated group were decreased compared with
SAP group. SOD activity was enhanced after UTI treatment
(59.72±5.45 vs. 48.32±3.81nmol/ml, P<0.01). Mitochondrial
function assays showed that MMP (17.30±1.60 vs. 46.94±3.49%,
P<0.01) and MPT (30.14±2.46 vs. 51.31±3.23%, P<0.01)
were inhibited by UTI. Conclusion: UTI reduces T lymphocytes apoptosis
and improves immunological function in SAP rats, possibly via enhancing
the scavenging capacity of oxygen free radical and attenuating the
influence of oxidative stress.]]></description><subject>Apoptosis</subject><subject>Mitochondrion</subject><subject>Severe acute pancreatitis</subject><subject>T cell</subject><subject>Ulinastatin</subject><issn>1596-5996</issn><issn>1596-9827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNpFkN1KAzEQhYMoWKt3PkAewF0zTbLZXJbiHxYUrdchm51gytpdkrTi27u1ojAww8w3Z5hDyCWwUnAQ13k9xHIHPECpjsgEpK4KXc_U8W8tta5OyVlKa8ZkpTVMyONbFzY2ZZvDhr5gu3WY6IousOvofOiH3KeQ6H5mc6KfIb_TV9xhRDp324z02W5cxHE7h3ROTrztEl785ilZ3d6sFvfF8unuYTFfFs1MiVyMl4WTrWW1Vlyja7TXTnAueSXrFhiIxkvHfQUeLNbe1nUrmVJCtthI4FNydZB1sU8pojdDDB82fhlgZu-D2ftgfnwwasTLA96EfvwV_2gXgzX_zTFAMKb4N-YUY3E</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Wang, Chunli</creator><creator>Ma, Tao</creator><creator>Zhou, Xiaolei</creator><creator>Li, Nan</creator><creator>You, Shengyi</creator><general>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</general><scope>RBI</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140101</creationdate><title>Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis</title><author>Wang, Chunli ; Ma, Tao ; Zhou, Xiaolei ; Li, Nan ; You, Shengyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b274t-5694c5da089739ecb9f9c43353658d1014bf5c3f61f1ae8fa88d507745deb513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Mitochondrion</topic><topic>Severe acute pancreatitis</topic><topic>T cell</topic><topic>Ulinastatin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Chunli</creatorcontrib><creatorcontrib>Ma, Tao</creatorcontrib><creatorcontrib>Zhou, Xiaolei</creatorcontrib><creatorcontrib>Li, Nan</creatorcontrib><creatorcontrib>You, Shengyi</creatorcontrib><collection>Bioline International</collection><collection>CrossRef</collection><jtitle>Tropical journal of pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Chunli</au><au>Ma, Tao</au><au>Zhou, Xiaolei</au><au>Li, Nan</au><au>You, Shengyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis</atitle><jtitle>Tropical journal of pharmaceutical research</jtitle><date>2014-01-01</date><risdate>2014</risdate><volume>13</volume><issue>1</issue><spage>47</spage><pages>47-</pages><issn>1596-5996</issn><eissn>1596-9827</eissn><abstract><![CDATA[Purpose: To investigate the immunoregulative effects of ulinastatin
(UTI) on T lymphocytes apoptosis in rats with severe acute pancreatitis
(SAP) and to elucidate its underlying molecular mechanism. Methods:
Thirty six Wistar rats were randomly divided into 3 groups (n =12):
sham, SAP model and UTI-treated group. SAP model was established by
intrapancreatobiliary duct injection of 5% sodium taurocholate. A bolus
of 10000 U/kg UTI was intravenously injected after SAP establishment. T
cell apoptosis was determined by Annexin-V/PI double-staining.
Oxidative stress was evaluated by examining changes in the levels of
reactive oxygen species (ROS). Total superoxide dismutase (SOD) in
serum was tested by hydroxylamine colorimetric assay, and
malondialdehyde levels were examined by thiobarbituric acid assay.
Mitochondrial function was evaluated by analyzing mitochondrial
membrane potential (MMP) and mitochondrial permeability transition pore
(MPTP). Results: We found CD4+ T cells (32.10±2.87% vs.
45.22±4.38%, P<0.01) and CD4+/CD8+ T cells in SAP rats
significantly decreased compared with sham group (1.15±0.12 vs.
2.23±0.12%, P<0.01), while the percent of the apoptotic CD4+
and (17.70±2.10 vs. 3.82±0.50%, P<0.01) CD8+ T lymphocytes
was highly increased (2.78±0.45 vs. 1.97±0.36%, P<0.01
compared with sham group). After UTI treatment, the apoptosis of CD4+ T
lymphocytes significantly decreased compared with SAP group
(8.58±1.09 vs. 17.70±2.10%, P<0.01), while the percent of
CD4+ T and CD4+/CD8+ lymphocytes significantly enhanced (P<0.01).
ROS (mean fluorescence intensity): 5107±430 vs. 12904±840,
P<0.01) and MDA levels (4.41±0.32 vs. 7.25±0.57nmol/ml,
P<0.01) in serum in UTI-treated group were decreased compared with
SAP group. SOD activity was enhanced after UTI treatment
(59.72±5.45 vs. 48.32±3.81nmol/ml, P<0.01). Mitochondrial
function assays showed that MMP (17.30±1.60 vs. 46.94±3.49%,
P<0.01) and MPT (30.14±2.46 vs. 51.31±3.23%, P<0.01)
were inhibited by UTI. Conclusion: UTI reduces T lymphocytes apoptosis
and improves immunological function in SAP rats, possibly via enhancing
the scavenging capacity of oxygen free radical and attenuating the
influence of oxidative stress.]]></abstract><pub>Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria</pub><doi>10.4314/tjpr.v13i1.7</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1596-5996 |
| ispartof | Tropical journal of pharmaceutical research, 2014-01, Vol.13 (1), p.47 |
| issn | 1596-5996 1596-9827 |
| language | eng |
| recordid | cdi_crossref_primary_10_4314_tjpr_v13i1_7 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; African Journals Online (Open Access); Bioline International; Free Full-Text Journals in Chemistry |
| subjects | Apoptosis Mitochondrion Severe acute pancreatitis T cell Ulinastatin |
| title | Ulinastatin Reduces T Cell Apoptosis in Rats with Severe Acute Pancreatitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T04%3A28%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-bioline_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ulinastatin%20Reduces%20T%20Cell%20Apoptosis%20in%20Rats%20with%20Severe%20Acute%20Pancreatitis&rft.jtitle=Tropical%20journal%20of%20pharmaceutical%20research&rft.au=Wang,%20Chunli&rft.date=2014-01-01&rft.volume=13&rft.issue=1&rft.spage=47&rft.pages=47-&rft.issn=1596-5996&rft.eissn=1596-9827&rft_id=info:doi/10.4314/tjpr.v13i1.7&rft_dat=%3Cbioline_cross%3Ecria_bioline_pr_pr14007%3C/bioline_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |