Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells
Stem cell-based therapy has the potential to treat an array of human diseases. However, to study the therapeutic potential and safety of these cells, a scalable cell culture medium is needed that is free of human or bovine-derived serum proteins. Thus, cost-effective recombinant serum proteins and c...
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| Veröffentlicht in: | Bioengineered 2014-05, Vol.5 (3), p.180-185 |
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| creator | Alfano, Randall Youngblood, Bradford A Zhang, Deshui Huang, Ning MacDonald, Clinton C |
| description | Stem cell-based therapy has the potential to treat an array of human diseases. However, to study the therapeutic potential and safety of these cells, a scalable cell culture medium is needed that is free of human or bovine-derived serum proteins. Thus, cost-effective recombinant serum proteins and cytokines are needed to produce such mediums. One such cytokine, leukemia inhibitory factor (LIF), has been shown to be a critical paracrine factor that maintains stem cell pluripotency in murine embryonic stem cells and human naïve stem cells while simultaneously inhibiting differentiation. We recently produced recombinant human LIF (rhLIF) in a rice-based protein expression system known as ExpressTec.
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We described expression of rice-derived rhLIF and demonstrated its biological equivalency to E. coli-derived rhLIF in traditional and embryonic mouse stem cell systems. Here we describe the expression yield of rice-derived rhLIF and the scale up production capacity. We provide further evidence of the efficacy of rice-derived rhLIF in additional stem cell systems including human neural stem cells and mouse induced pluripotent stem (iPS) cells. The expression level, biological activity, and potential for production at commercial scale of rice-derived rhLIF provides a proof-of-principal for ExpressTec-derived proteins to produce regulatory-friendly, high performance, and dependable stem cell media. |
| doi_str_mv | 10.4161/bioe.28996 |
| format | Article |
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12
We described expression of rice-derived rhLIF and demonstrated its biological equivalency to E. coli-derived rhLIF in traditional and embryonic mouse stem cell systems. Here we describe the expression yield of rice-derived rhLIF and the scale up production capacity. We provide further evidence of the efficacy of rice-derived rhLIF in additional stem cell systems including human neural stem cells and mouse induced pluripotent stem (iPS) cells. The expression level, biological activity, and potential for production at commercial scale of rice-derived rhLIF provides a proof-of-principal for ExpressTec-derived proteins to produce regulatory-friendly, high performance, and dependable stem cell media.</description><identifier>ISSN: 2165-5979</identifier><identifier>EISSN: 2165-5987</identifier><identifier>DOI: 10.4161/bioe.28996</identifier><identifier>PMID: 24776984</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Addendum ; Animals ; Biomarkers - metabolism ; Cell Differentiation - drug effects ; Codon ; embryonic stem cell ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Gene Expression ; Glycosylation ; growth rate ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - drug effects ; Induced Pluripotent Stem Cells - metabolism ; Leukemia Inhibitory Factor - biosynthesis ; Leukemia Inhibitory Factor - genetics ; Leukemia Inhibitory Factor - pharmacology ; LIF ; Mice ; Nanog ; Nanog Homeobox Protein ; Neural Stem Cells - cytology ; Neural Stem Cells - drug effects ; Neural Stem Cells - metabolism ; Oct 4 ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Oryza - genetics ; Oryza - metabolism ; Promoter Regions, Genetic ; recombinant protein expression ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Recombinant Proteins - pharmacology</subject><ispartof>Bioengineered, 2014-05, Vol.5 (3), p.180-185</ispartof><rights>Copyright © 2014 Landes Bioscience 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-e405e50f6909f2edf9fafe663ef9fd5f500461f7819abb2668b66bf2828fe08e3</citedby><cites>FETCH-LOGICAL-c424t-e405e50f6909f2edf9fafe663ef9fd5f500461f7819abb2668b66bf2828fe08e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101010/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4101010/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24776984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alfano, Randall</creatorcontrib><creatorcontrib>Youngblood, Bradford A</creatorcontrib><creatorcontrib>Zhang, Deshui</creatorcontrib><creatorcontrib>Huang, Ning</creatorcontrib><creatorcontrib>MacDonald, Clinton C</creatorcontrib><title>Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells</title><title>Bioengineered</title><addtitle>Bioengineered</addtitle><description>Stem cell-based therapy has the potential to treat an array of human diseases. However, to study the therapeutic potential and safety of these cells, a scalable cell culture medium is needed that is free of human or bovine-derived serum proteins. Thus, cost-effective recombinant serum proteins and cytokines are needed to produce such mediums. One such cytokine, leukemia inhibitory factor (LIF), has been shown to be a critical paracrine factor that maintains stem cell pluripotency in murine embryonic stem cells and human naïve stem cells while simultaneously inhibiting differentiation. We recently produced recombinant human LIF (rhLIF) in a rice-based protein expression system known as ExpressTec.
12
We described expression of rice-derived rhLIF and demonstrated its biological equivalency to E. coli-derived rhLIF in traditional and embryonic mouse stem cell systems. Here we describe the expression yield of rice-derived rhLIF and the scale up production capacity. We provide further evidence of the efficacy of rice-derived rhLIF in additional stem cell systems including human neural stem cells and mouse induced pluripotent stem (iPS) cells. The expression level, biological activity, and potential for production at commercial scale of rice-derived rhLIF provides a proof-of-principal for ExpressTec-derived proteins to produce regulatory-friendly, high performance, and dependable stem cell media.</description><subject>Addendum</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Codon</subject><subject>embryonic stem cell</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Gene Expression</subject><subject>Glycosylation</subject><subject>growth rate</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - drug effects</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Leukemia Inhibitory Factor - biosynthesis</subject><subject>Leukemia Inhibitory Factor - genetics</subject><subject>Leukemia Inhibitory Factor - pharmacology</subject><subject>LIF</subject><subject>Mice</subject><subject>Nanog</subject><subject>Nanog Homeobox Protein</subject><subject>Neural Stem Cells - cytology</subject><subject>Neural Stem Cells - drug effects</subject><subject>Neural Stem Cells - metabolism</subject><subject>Oct 4</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant protein expression</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - pharmacology</subject><issn>2165-5979</issn><issn>2165-5987</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><recordid>eNptkUFr3DAQhU1paEKSS39AmWNa2K3klWX7UighaQoLuSRnIdujWq0luZK8rfub8iMrZ9MlhaDDDOjjvTe8LHtLyZpRTj822uE6r-qav8pOcsqLVVFX5evDXtbH2XkI3wkhlGxYUVZvsuOclSWvK3aSPdxMRloYcPqBRkvQtteNjs7PoGSbJozedVOLHTQzxB7h6vfoMYQ7bMFg7F0HyjsDXrcIF7d-_iMhyKh3Erbr96ADJBm9w6QM_aOXxcnLAUJEAy0OQyJsB8ZNYYH2XuMweT26iDY-A8-yIyWHgOdP8zS7v766u7xZbW-_fL38vF21LGdxhYwUWBDFa1KrHDtVK6mQ8w2mrStUQQjjVJUVrWXT5JxXDeeNyqu8Ukgq3Jxmn_a649QY7NqUIiUWo9dG-lk4qcX_P1b34pvbCUbJ8pLAxZOAdz8nDFEYHZYTpMV0p6AF4zlN_S3ohz3aeheCR3WwoUQsDYulYfHYcILfPQ92QP_1mYBiD2irnDfyl_NDJ6KcB-eVl7bVQWxeEP4Lg6i5KA</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Alfano, Randall</creator><creator>Youngblood, Bradford A</creator><creator>Zhang, Deshui</creator><creator>Huang, Ning</creator><creator>MacDonald, Clinton C</creator><general>Taylor & Francis</general><general>Landes Bioscience</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells</title><author>Alfano, Randall ; Youngblood, Bradford A ; Zhang, Deshui ; Huang, Ning ; MacDonald, Clinton C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-e405e50f6909f2edf9fafe663ef9fd5f500461f7819abb2668b66bf2828fe08e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Addendum</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Codon</topic><topic>embryonic stem cell</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Gene Expression</topic><topic>Glycosylation</topic><topic>growth rate</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Induced Pluripotent Stem Cells - cytology</topic><topic>Induced Pluripotent Stem Cells - drug effects</topic><topic>Induced Pluripotent Stem Cells - metabolism</topic><topic>Leukemia Inhibitory Factor - biosynthesis</topic><topic>Leukemia Inhibitory Factor - genetics</topic><topic>Leukemia Inhibitory Factor - pharmacology</topic><topic>LIF</topic><topic>Mice</topic><topic>Nanog</topic><topic>Nanog Homeobox Protein</topic><topic>Neural Stem Cells - cytology</topic><topic>Neural Stem Cells - drug effects</topic><topic>Neural Stem Cells - metabolism</topic><topic>Oct 4</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant protein expression</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alfano, Randall</creatorcontrib><creatorcontrib>Youngblood, Bradford A</creatorcontrib><creatorcontrib>Zhang, Deshui</creatorcontrib><creatorcontrib>Huang, Ning</creatorcontrib><creatorcontrib>MacDonald, Clinton C</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bioengineered</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alfano, Randall</au><au>Youngblood, Bradford A</au><au>Zhang, Deshui</au><au>Huang, Ning</au><au>MacDonald, Clinton C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells</atitle><jtitle>Bioengineered</jtitle><addtitle>Bioengineered</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>5</volume><issue>3</issue><spage>180</spage><epage>185</epage><pages>180-185</pages><issn>2165-5979</issn><eissn>2165-5987</eissn><abstract>Stem cell-based therapy has the potential to treat an array of human diseases. However, to study the therapeutic potential and safety of these cells, a scalable cell culture medium is needed that is free of human or bovine-derived serum proteins. Thus, cost-effective recombinant serum proteins and cytokines are needed to produce such mediums. One such cytokine, leukemia inhibitory factor (LIF), has been shown to be a critical paracrine factor that maintains stem cell pluripotency in murine embryonic stem cells and human naïve stem cells while simultaneously inhibiting differentiation. We recently produced recombinant human LIF (rhLIF) in a rice-based protein expression system known as ExpressTec.
12
We described expression of rice-derived rhLIF and demonstrated its biological equivalency to E. coli-derived rhLIF in traditional and embryonic mouse stem cell systems. Here we describe the expression yield of rice-derived rhLIF and the scale up production capacity. We provide further evidence of the efficacy of rice-derived rhLIF in additional stem cell systems including human neural stem cells and mouse induced pluripotent stem (iPS) cells. The expression level, biological activity, and potential for production at commercial scale of rice-derived rhLIF provides a proof-of-principal for ExpressTec-derived proteins to produce regulatory-friendly, high performance, and dependable stem cell media.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>24776984</pmid><doi>10.4161/bioe.28996</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Addendum Animals Biomarkers - metabolism Cell Differentiation - drug effects Codon embryonic stem cell Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Glycosylation growth rate Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - drug effects Induced Pluripotent Stem Cells - metabolism Leukemia Inhibitory Factor - biosynthesis Leukemia Inhibitory Factor - genetics Leukemia Inhibitory Factor - pharmacology LIF Mice Nanog Nanog Homeobox Protein Neural Stem Cells - cytology Neural Stem Cells - drug effects Neural Stem Cells - metabolism Oct 4 Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Oryza - genetics Oryza - metabolism Promoter Regions, Genetic recombinant protein expression Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - pharmacology |
| title | Human leukemia inhibitory factor produced by the ExpressTec method from rice (Oryza sativa L.) is active in human neural stem cells and mouse induced pluripotent stem cells |
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