Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib
To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution t...
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| Veröffentlicht in: | Bioanalysis 2022-01, Vol.14 (1), p.29-45 |
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| creator | Padya, Bharath S Hegde, Aswathi R Mutalik, Sadhana P Biswas, Swati Mutalik, Srinivas |
| description | To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG).
Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique.
Separation of the analytes was effected on a Luna
C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid–liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats.
The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix. |
| doi_str_mv | 10.4155/bio-2021-0212 |
| format | Article |
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Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique.
Separation of the analytes was effected on a Luna
C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid–liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats.
The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.</description><identifier>ISSN: 1757-6180</identifier><identifier>EISSN: 1757-6199</identifier><identifier>DOI: 10.4155/bio-2021-0212</identifier><identifier>PMID: 34786955</identifier><language>eng</language><publisher>England: Newlands Press Ltd</publisher><subject>5-fluorouracil ; Animals ; bioanalytical ; Biphenyl Compounds - pharmacokinetics ; Chromatography, High Pressure Liquid - methods ; Fluorouracil - pharmacokinetics ; Male ; Neoplasms - drug therapy ; Pyridines - pharmacokinetics ; Rats ; Rats, Wistar ; RP-HPLC ; simultaneous estimation ; sonidegib</subject><ispartof>Bioanalysis, 2022-01, Vol.14 (1), p.29-45</ispartof><rights>2021 Newlands Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-54730ea919e008953b94af2b2e76134cb09325a87fbc2e13c32a02341bd647fc3</citedby><cites>FETCH-LOGICAL-c343t-54730ea919e008953b94af2b2e76134cb09325a87fbc2e13c32a02341bd647fc3</cites><orcidid>0000-0002-0642-1928</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34786955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Padya, Bharath S</creatorcontrib><creatorcontrib>Hegde, Aswathi R</creatorcontrib><creatorcontrib>Mutalik, Sadhana P</creatorcontrib><creatorcontrib>Biswas, Swati</creatorcontrib><creatorcontrib>Mutalik, Srinivas</creatorcontrib><title>Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib</title><title>Bioanalysis</title><addtitle>Bioanalysis</addtitle><description>To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG).
Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique.
Separation of the analytes was effected on a Luna
C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid–liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats.
The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.</description><subject>5-fluorouracil</subject><subject>Animals</subject><subject>bioanalytical</subject><subject>Biphenyl Compounds - pharmacokinetics</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fluorouracil - pharmacokinetics</subject><subject>Male</subject><subject>Neoplasms - drug therapy</subject><subject>Pyridines - pharmacokinetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RP-HPLC</subject><subject>simultaneous estimation</subject><subject>sonidegib</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMottQevUr-QDSfu5tjKWqFgh70vCTZRCO7m5JsDv33pqwfJwNDZoZnXmZeAK4JvuVEiDvtA6KYElSCnoElqUWNKiLl-W_e4AVYp_SJy2O0kVxeggXjdVNJIZag24yqP07eqB6qsYNFUf11di_7LRzs9BE66EKEyQ-5n9RoQ07QpskPavJhhMFBgVyfQww5KuNnrRRG39l3r6_AhVN9suvvfwXeHu5ftzu0f3582m72yDDOJiR4zbBVkkiLcSMF05IrRzW1dUUYNxpLRoVqaqcNtYQZRhWmjBPdVbx2hq0AmnVNDClF69pDLCvGY0twezKsLee1J8Pak2GFv5n5Q9aD7X7pH3sKIGfA5SlHm4y3o7HtXJUJb_xo_xH_AtXheqE</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Padya, Bharath S</creator><creator>Hegde, Aswathi R</creator><creator>Mutalik, Sadhana P</creator><creator>Biswas, Swati</creator><creator>Mutalik, Srinivas</creator><general>Newlands Press Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-0642-1928</orcidid></search><sort><creationdate>20220101</creationdate><title>Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib</title><author>Padya, Bharath S ; Hegde, Aswathi R ; Mutalik, Sadhana P ; Biswas, Swati ; Mutalik, Srinivas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-54730ea919e008953b94af2b2e76134cb09325a87fbc2e13c32a02341bd647fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>5-fluorouracil</topic><topic>Animals</topic><topic>bioanalytical</topic><topic>Biphenyl Compounds - pharmacokinetics</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fluorouracil - pharmacokinetics</topic><topic>Male</topic><topic>Neoplasms - drug therapy</topic><topic>Pyridines - pharmacokinetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RP-HPLC</topic><topic>simultaneous estimation</topic><topic>sonidegib</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Padya, Bharath S</creatorcontrib><creatorcontrib>Hegde, Aswathi R</creatorcontrib><creatorcontrib>Mutalik, Sadhana P</creatorcontrib><creatorcontrib>Biswas, Swati</creatorcontrib><creatorcontrib>Mutalik, Srinivas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Padya, Bharath S</au><au>Hegde, Aswathi R</au><au>Mutalik, Sadhana P</au><au>Biswas, Swati</au><au>Mutalik, Srinivas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib</atitle><jtitle>Bioanalysis</jtitle><addtitle>Bioanalysis</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>14</volume><issue>1</issue><spage>29</spage><epage>45</epage><pages>29-45</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG).
Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique.
Separation of the analytes was effected on a Luna
C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid–liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats.
The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.</abstract><cop>England</cop><pub>Newlands Press Ltd</pub><pmid>34786955</pmid><doi>10.4155/bio-2021-0212</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-0642-1928</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1757-6180 |
| ispartof | Bioanalysis, 2022-01, Vol.14 (1), p.29-45 |
| issn | 1757-6180 1757-6199 |
| language | eng |
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| source | MEDLINE; PubMed Central |
| subjects | 5-fluorouracil Animals bioanalytical Biphenyl Compounds - pharmacokinetics Chromatography, High Pressure Liquid - methods Fluorouracil - pharmacokinetics Male Neoplasms - drug therapy Pyridines - pharmacokinetics Rats Rats, Wistar RP-HPLC simultaneous estimation sonidegib |
| title | Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib |
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