A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso Scale Discovery platform
Monitoring appearance of neutralizing antibodies (NAbs) to multidomain large molecule drugs is a challenging task. Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for...
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| Veröffentlicht in: | Bioanalysis 2021-11, Vol.13 (22), p.1659-1669 |
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| container_title | Bioanalysis |
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| creator | Ablamunits, Vitaly Basak, Soma Lawrence-Henderson, Rosemary Caiazzo, Teresa M Kamerud, John |
| description | Monitoring appearance of neutralizing antibodies (NAbs) to multidomain large molecule drugs is a challenging task.
Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample.
The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 μg/ml and to the level of soluble target(s) >400 ng/ml.
Our data suggest that multiplex approach can be successfully used for development of NAb assays in competitive ligand-binding assay format. |
| doi_str_mv | 10.4155/bio-2021-0177 |
| format | Article |
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Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample.
The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 μg/ml and to the level of soluble target(s) >400 ng/ml.
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Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample.
The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 μg/ml and to the level of soluble target(s) >400 ng/ml.
Our data suggest that multiplex approach can be successfully used for development of NAb assays in competitive ligand-binding assay format.</description><subject>bispecific drug</subject><subject>competitive ligand-binding assay</subject><subject>neutralizing antibodies</subject><subject>U-plex MSD</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kMlOAzEMQCMEEhX0yD0_EEhmy8yxKqtUxAE4j7I4ldFsSjIV5Q_4a2ZaxI1cHMvPlv0IuRL8OhN5fqOxZwlPBONCyhOyEDKXrBBVdfr3L_k5WYbwwaeXJmWVVQvyvaKmbweIGHEHtMGt6izT2FnstlSFoPY09tRCBBNpB2P0qsGvQ7GLqHuLEGZCUY1hAIMODbV-3NIxHCjajk3EoYFP-gyhp69GNUBvMZh-B35Ph0ZF1_v2kpw51QRY_sYL8n5_97Z-ZJuXh6f1asNMkuaRTYuXmpem1EbJ3Ak-HZ2KojI6U0JWmYJUpGVSuFSUUha2tCYDyJSSTgsLMr0g7DjX-D4ED64ePLbK72vB61llPamsZ5X1rHLiqyPvxjh6CAahM1AfsxYsGuzgn94fcKJ9ow</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Ablamunits, Vitaly</creator><creator>Basak, Soma</creator><creator>Lawrence-Henderson, Rosemary</creator><creator>Caiazzo, Teresa M</creator><creator>Kamerud, John</creator><general>Newlands Press Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3582-3600</orcidid><orcidid>https://orcid.org/0000-0002-0769-1559</orcidid></search><sort><creationdate>20211101</creationdate><title>A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso Scale Discovery platform</title><author>Ablamunits, Vitaly ; Basak, Soma ; Lawrence-Henderson, Rosemary ; Caiazzo, Teresa M ; Kamerud, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c235t-3288b08c8bca75f102023169cb4a1794ae313826f318776d8dc4ee4aa7fb1de73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>bispecific drug</topic><topic>competitive ligand-binding assay</topic><topic>neutralizing antibodies</topic><topic>U-plex MSD</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ablamunits, Vitaly</creatorcontrib><creatorcontrib>Basak, Soma</creatorcontrib><creatorcontrib>Lawrence-Henderson, Rosemary</creatorcontrib><creatorcontrib>Caiazzo, Teresa M</creatorcontrib><creatorcontrib>Kamerud, John</creatorcontrib><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ablamunits, Vitaly</au><au>Basak, Soma</au><au>Lawrence-Henderson, Rosemary</au><au>Caiazzo, Teresa M</au><au>Kamerud, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso Scale Discovery platform</atitle><jtitle>Bioanalysis</jtitle><date>2021-11-01</date><risdate>2021</risdate><volume>13</volume><issue>22</issue><spage>1659</spage><epage>1669</epage><pages>1659-1669</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>Monitoring appearance of neutralizing antibodies (NAbs) to multidomain large molecule drugs is a challenging task.
Here, we report development of a competitive ligand-binding assay for detection of NAbs to a bispecific candidate drug using a multiplex Meso Scale Discovery platform, which allows for detection of NAbs to both drug arms in the same sample.
The assay has sensitivity better than 250 ng/ml and is tolerant to the presence of drug at concentration >600 μg/ml and to the level of soluble target(s) >400 ng/ml.
Our data suggest that multiplex approach can be successfully used for development of NAb assays in competitive ligand-binding assay format.</abstract><pub>Newlands Press Ltd</pub><doi>10.4155/bio-2021-0177</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3582-3600</orcidid><orcidid>https://orcid.org/0000-0002-0769-1559</orcidid></addata></record> |
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| ispartof | Bioanalysis, 2021-11, Vol.13 (22), p.1659-1669 |
| issn | 1757-6180 1757-6199 |
| language | eng |
| recordid | cdi_crossref_primary_10_4155_bio_2021_0177 |
| source | PubMed Central |
| subjects | bispecific drug competitive ligand-binding assay neutralizing antibodies U-plex MSD |
| title | A competitive ligand-binding assay to detect neutralizing antibodies to a bispecific drug using a multiplex Meso Scale Discovery platform |
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