A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS
An LC-MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys. Assay cali...
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| Veröffentlicht in: | Bioanalysis 2018-03, Vol.10 (5), p.357-368 |
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| creator | King-Ahmad, Amanda J Kalgutkar, Amit S Niosi, Mark Eng, Heather Holliman, Christopher |
| description | An LC-MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys.
Assay calibration curves exhibited a linear dynamic range of 10-5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys.
The dynamic range of our LC-MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans. |
| doi_str_mv | 10.4155/bio-2017-0239 |
| format | Article |
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Assay calibration curves exhibited a linear dynamic range of 10-5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys.
The dynamic range of our LC-MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans.</description><identifier>ISSN: 1757-6180</identifier><identifier>EISSN: 1757-6199</identifier><identifier>DOI: 10.4155/bio-2017-0239</identifier><identifier>PMID: 29516741</identifier><language>eng</language><publisher>England: Future Science Ltd</publisher><subject>agonist ; bioanalytical ; ELISA ; enzyme-linked immunosorbent assay ; GLP-1 ; glucagon-like peptide-1 ; LC-MS/MS ; liquid chromatography tandem mass Spectrometry ; liraglutide ; monkey ; pharmacokinetics ; plasma ; receptor</subject><ispartof>Bioanalysis, 2018-03, Vol.10 (5), p.357-368</ispartof><rights>2018 Newlands Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-2fa2efa3dc5b1918a568ec89827e9796c588e6a231a82eab482c85529d7362dc3</citedby><cites>FETCH-LOGICAL-c343t-2fa2efa3dc5b1918a568ec89827e9796c588e6a231a82eab482c85529d7362dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29516741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>King-Ahmad, Amanda J</creatorcontrib><creatorcontrib>Kalgutkar, Amit S</creatorcontrib><creatorcontrib>Niosi, Mark</creatorcontrib><creatorcontrib>Eng, Heather</creatorcontrib><creatorcontrib>Holliman, Christopher</creatorcontrib><title>A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS</title><title>Bioanalysis</title><addtitle>Bioanalysis</addtitle><description>An LC-MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys.
Assay calibration curves exhibited a linear dynamic range of 10-5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys.
The dynamic range of our LC-MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans.</description><subject>agonist</subject><subject>bioanalytical</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>GLP-1</subject><subject>glucagon-like peptide-1</subject><subject>LC-MS/MS</subject><subject>liquid chromatography tandem mass Spectrometry</subject><subject>liraglutide</subject><subject>monkey</subject><subject>pharmacokinetics</subject><subject>plasma</subject><subject>receptor</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kM1u1TAQRi0EaqvSZbfIL2CInTixl1XFn1TEgnYdTZzJvQOJHWwHqW_EY-JwoazwxmN_x5-sw9i1rF43Uus3AwWhKtmJStX2GbuQne5EK619_jSb6pxdpfS1KqtWxjb2jJ0rq2XbNfKC_bzhCX2iTD-QL5iPYeRTiDwfkX_fwGfKkCl4HqbfdyuumUYUAyQc-WHeHByCFzN9-5dJHtGVudTsIaXMZ4pQ4D3m5Pk6Q1qAb4n8gS_kYpjmjUZyibtjDAvkcIiwHh95Bj_iwj99ecleTDAnvPqzX7KHd2_vbz-Iu8_vP97e3AlXN3UWagKFE9Sj04O00oBuDTpjjerQdrZ12hhsQdUSjEIYGqOc0VrZsatbNbr6kolTb_lUShGnfo20QHzsZdXv0vsivd-l97v0wr868es2LDg-0X8VF8CegGnLW8TkCL3D_nQqL8iRx_-U_wKO85VF</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>King-Ahmad, Amanda J</creator><creator>Kalgutkar, Amit S</creator><creator>Niosi, Mark</creator><creator>Eng, Heather</creator><creator>Holliman, Christopher</creator><general>Future Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180301</creationdate><title>A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS</title><author>King-Ahmad, Amanda J ; Kalgutkar, Amit S ; Niosi, Mark ; Eng, Heather ; Holliman, Christopher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-2fa2efa3dc5b1918a568ec89827e9796c588e6a231a82eab482c85529d7362dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>agonist</topic><topic>bioanalytical</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>GLP-1</topic><topic>glucagon-like peptide-1</topic><topic>LC-MS/MS</topic><topic>liquid chromatography tandem mass Spectrometry</topic><topic>liraglutide</topic><topic>monkey</topic><topic>pharmacokinetics</topic><topic>plasma</topic><topic>receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>King-Ahmad, Amanda J</creatorcontrib><creatorcontrib>Kalgutkar, Amit S</creatorcontrib><creatorcontrib>Niosi, Mark</creatorcontrib><creatorcontrib>Eng, Heather</creatorcontrib><creatorcontrib>Holliman, Christopher</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>King-Ahmad, Amanda J</au><au>Kalgutkar, Amit S</au><au>Niosi, Mark</au><au>Eng, Heather</au><au>Holliman, Christopher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS</atitle><jtitle>Bioanalysis</jtitle><addtitle>Bioanalysis</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>10</volume><issue>5</issue><spage>357</spage><epage>368</epage><pages>357-368</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>An LC-MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys.
Assay calibration curves exhibited a linear dynamic range of 10-5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys.
The dynamic range of our LC-MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans.</abstract><cop>England</cop><pub>Future Science Ltd</pub><pmid>29516741</pmid><doi>10.4155/bio-2017-0239</doi><tpages>12</tpages></addata></record> |
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| source | PubMed Central |
| subjects | agonist bioanalytical ELISA enzyme-linked immunosorbent assay GLP-1 glucagon-like peptide-1 LC-MS/MS liquid chromatography tandem mass Spectrometry liraglutide monkey pharmacokinetics plasma receptor |
| title | A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS |
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