Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms

Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices; ELISA, Meso Scale Discovery, Gyrolab xP Workstation and Simoa HD-1. Results & methodology: After completion of custom assay development, the single-molecule...

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Veröffentlicht in:	Bioanalysis 2016-03, Vol.8 (6), p.511-518
Hauptverfasser:	Myzithras, Maria, Li, Hua, Bigwarfe, Tammy, Waltz, Erica, Gupta, Priyanka, Low, Sarah, Hayes, David B, MacDonnell, Scott, Ahlberg, Jennifer, Franti, Michael, Roberts, Simon
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - immunology Biotinylation Bone Morphogenetic Proteins - analysis Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism Enzyme-Linked Immunosorbent Assay - economics Enzyme-Linked Immunosorbent Assay - methods Growth Differentiation Factors - analysis Growth Differentiation Factors - genetics Growth Differentiation Factors - metabolism Humans Limit of Detection Mice Rats Recombinant Proteins - analysis Recombinant Proteins - biosynthesis Recombinant Proteins - immunology
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