Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms
Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices; ELISA, Meso Scale Discovery, Gyrolab xP Workstation and Simoa HD-1. Results & methodology: After completion of custom assay development, the single-molecule...
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| Veröffentlicht in: | Bioanalysis 2016-03, Vol.8 (6), p.511-518 |
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| creator | Myzithras, Maria Li, Hua Bigwarfe, Tammy Waltz, Erica Gupta, Priyanka Low, Sarah Hayes, David B MacDonnell, Scott Ahlberg, Jennifer Franti, Michael Roberts, Simon |
| description | Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices; ELISA, Meso Scale Discovery, Gyrolab xP Workstation and Simoa HD-1. Results & methodology: After completion of custom assay development, the single-molecule ELISA (Simoa) achieved the greatest sensitivity with a lower limit of quantitation of 0.1 ng/ml, an improvement of 100-fold over the next sensitive platform (MSD).
This improvement was essential to enable detection of GDF11 in biological samples, and without the technology the sensitivity achieved on the other platforms would not have been sufficient. Other factors such as ease of use, cost, assay time and automation capability can also be considered when developing custom immunoassays, based on the requirements of the bioanalyst. |
| doi_str_mv | 10.4155/bio.16.17 |
| format | Article |
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This improvement was essential to enable detection of GDF11 in biological samples, and without the technology the sensitivity achieved on the other platforms would not have been sufficient. Other factors such as ease of use, cost, assay time and automation capability can also be considered when developing custom immunoassays, based on the requirements of the bioanalyst.</description><identifier>ISSN: 1757-6180</identifier><identifier>EISSN: 1757-6199</identifier><identifier>DOI: 10.4155/bio.16.17</identifier><identifier>PMID: 26917343</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Antibodies - immunology ; Biotinylation ; Bone Morphogenetic Proteins - analysis ; Bone Morphogenetic Proteins - genetics ; Bone Morphogenetic Proteins - metabolism ; Enzyme-Linked Immunosorbent Assay - economics ; Enzyme-Linked Immunosorbent Assay - methods ; Growth Differentiation Factors - analysis ; Growth Differentiation Factors - genetics ; Growth Differentiation Factors - metabolism ; Humans ; Limit of Detection ; Mice ; Rats ; Recombinant Proteins - analysis ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - immunology</subject><ispartof>Bioanalysis, 2016-03, Vol.8 (6), p.511-518</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c285t-78d73de0110e58ee2291fd86e5dd7851f7a9d8bbe59b027089bd0960560eade83</citedby><cites>FETCH-LOGICAL-c285t-78d73de0110e58ee2291fd86e5dd7851f7a9d8bbe59b027089bd0960560eade83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26917343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Myzithras, Maria</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Bigwarfe, Tammy</creatorcontrib><creatorcontrib>Waltz, Erica</creatorcontrib><creatorcontrib>Gupta, Priyanka</creatorcontrib><creatorcontrib>Low, Sarah</creatorcontrib><creatorcontrib>Hayes, David B</creatorcontrib><creatorcontrib>MacDonnell, Scott</creatorcontrib><creatorcontrib>Ahlberg, Jennifer</creatorcontrib><creatorcontrib>Franti, Michael</creatorcontrib><creatorcontrib>Roberts, Simon</creatorcontrib><title>Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms</title><title>Bioanalysis</title><addtitle>Bioanalysis</addtitle><description>Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices; ELISA, Meso Scale Discovery, Gyrolab xP Workstation and Simoa HD-1. Results & methodology: After completion of custom assay development, the single-molecule ELISA (Simoa) achieved the greatest sensitivity with a lower limit of quantitation of 0.1 ng/ml, an improvement of 100-fold over the next sensitive platform (MSD).
This improvement was essential to enable detection of GDF11 in biological samples, and without the technology the sensitivity achieved on the other platforms would not have been sufficient. Other factors such as ease of use, cost, assay time and automation capability can also be considered when developing custom immunoassays, based on the requirements of the bioanalyst.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Biotinylation</subject><subject>Bone Morphogenetic Proteins - analysis</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Bone Morphogenetic Proteins - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay - economics</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Growth Differentiation Factors - analysis</subject><subject>Growth Differentiation Factors - genetics</subject><subject>Growth Differentiation Factors - metabolism</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Mice</subject><subject>Rats</subject><subject>Recombinant Proteins - analysis</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - immunology</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kDFPwzAQhS0EolXpwB9AXhlSfEkd22yopQWpEgMwR5f4IhklcRS7lfrvCRR6y7vh6enTx9gtiMUSpHwonV9AvgB1waagpEpyMOby_GsxYfMQvsR4WarN0lyzSZobUNkym7K4pgM1vm-pi9zXHDu-b-KASaAuuOgOxN9d65FjCHjk0XPqsGyIb9cbAG4pUhWd7x458sq3PQ4u-I5jNfgQ-MiGHTbH6CpseN9grP3Qhht2VWMTaP6XM_a5ef5YvSS7t-3r6mmXVKmWMVHaqsySABAkNVGaGqitzklaq7SEWqGxuixJmlKkSmhTWmFyIXNBaElnM3Z_2v2lGagu-sG1OBwLEMWPvGIELCAvRhkzdnfq9vuyJXtu_qvKvgEo6Gvb</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Myzithras, Maria</creator><creator>Li, Hua</creator><creator>Bigwarfe, Tammy</creator><creator>Waltz, Erica</creator><creator>Gupta, Priyanka</creator><creator>Low, Sarah</creator><creator>Hayes, David B</creator><creator>MacDonnell, Scott</creator><creator>Ahlberg, Jennifer</creator><creator>Franti, Michael</creator><creator>Roberts, Simon</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20160301</creationdate><title>Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms</title><author>Myzithras, Maria ; Li, Hua ; Bigwarfe, Tammy ; Waltz, Erica ; Gupta, Priyanka ; Low, Sarah ; Hayes, David B ; MacDonnell, Scott ; Ahlberg, Jennifer ; Franti, Michael ; Roberts, Simon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c285t-78d73de0110e58ee2291fd86e5dd7851f7a9d8bbe59b027089bd0960560eade83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Biotinylation</topic><topic>Bone Morphogenetic Proteins - analysis</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Bone Morphogenetic Proteins - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay - economics</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Growth Differentiation Factors - analysis</topic><topic>Growth Differentiation Factors - genetics</topic><topic>Growth Differentiation Factors - metabolism</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Mice</topic><topic>Rats</topic><topic>Recombinant Proteins - analysis</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Myzithras, Maria</creatorcontrib><creatorcontrib>Li, Hua</creatorcontrib><creatorcontrib>Bigwarfe, Tammy</creatorcontrib><creatorcontrib>Waltz, Erica</creatorcontrib><creatorcontrib>Gupta, Priyanka</creatorcontrib><creatorcontrib>Low, Sarah</creatorcontrib><creatorcontrib>Hayes, David B</creatorcontrib><creatorcontrib>MacDonnell, Scott</creatorcontrib><creatorcontrib>Ahlberg, Jennifer</creatorcontrib><creatorcontrib>Franti, Michael</creatorcontrib><creatorcontrib>Roberts, Simon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Myzithras, Maria</au><au>Li, Hua</au><au>Bigwarfe, Tammy</au><au>Waltz, Erica</au><au>Gupta, Priyanka</au><au>Low, Sarah</au><au>Hayes, David B</au><au>MacDonnell, Scott</au><au>Ahlberg, Jennifer</au><au>Franti, Michael</au><au>Roberts, Simon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms</atitle><jtitle>Bioanalysis</jtitle><addtitle>Bioanalysis</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>8</volume><issue>6</issue><spage>511</spage><epage>518</epage><pages>511-518</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices; ELISA, Meso Scale Discovery, Gyrolab xP Workstation and Simoa HD-1. Results & methodology: After completion of custom assay development, the single-molecule ELISA (Simoa) achieved the greatest sensitivity with a lower limit of quantitation of 0.1 ng/ml, an improvement of 100-fold over the next sensitive platform (MSD).
This improvement was essential to enable detection of GDF11 in biological samples, and without the technology the sensitivity achieved on the other platforms would not have been sufficient. Other factors such as ease of use, cost, assay time and automation capability can also be considered when developing custom immunoassays, based on the requirements of the bioanalyst.</abstract><cop>England</cop><pmid>26917343</pmid><doi>10.4155/bio.16.17</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Bioanalysis, 2016-03, Vol.8 (6), p.511-518 |
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| source | MEDLINE; PubMed Central |
| subjects | Animals Antibodies - immunology Biotinylation Bone Morphogenetic Proteins - analysis Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism Enzyme-Linked Immunosorbent Assay - economics Enzyme-Linked Immunosorbent Assay - methods Growth Differentiation Factors - analysis Growth Differentiation Factors - genetics Growth Differentiation Factors - metabolism Humans Limit of Detection Mice Rats Recombinant Proteins - analysis Recombinant Proteins - biosynthesis Recombinant Proteins - immunology |
| title | Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms |
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