Development and characterization of antibody reagents to assess anti-PEG IgG antibodies in clinical samples
Polyethylene glycol (PEG) is a polymer that can be conjugated with therapeutic proteins. Monitoring anti-PEG antibodies in human subjects may be required as part of immunogenicity assessment. The lack of well-characterized anti-PEG reagents have limited our understanding of anti-PEG humoral response...
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| Veröffentlicht in: | Bioanalysis 2015-01, Vol.7 (15), p.1869-1883 |
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| container_title | Bioanalysis |
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| creator | Krishna, Murli Palme, Holly Duo, Jia Lin, Zheng Corbett, Martin Dodge, Robert Piccoli, Steven Myler, Heather Pillutla, Renuka Desilva, Binodh |
| description | Polyethylene glycol (PEG) is a polymer that can be conjugated with therapeutic proteins. Monitoring anti-PEG antibodies in human subjects may be required as part of immunogenicity assessment. The lack of well-characterized anti-PEG reagents have limited our understanding of anti-PEG humoral response.
Antibodies reactive to PEG were engineered with a human IgG1 Fc. Surface plasmon resonance and plate-based methods demonstrated that their binding was dependent on molecular weight (MW) of PEG. Specificity experiments using chemical analogs identified their specificity.
Affinity, specificity and MW of PEG are critical characteristics that impact interactions of anti-PEG antibodies with PEG. These attributes especially MW of PEG and the assay formats may impact the ability to detect anti-PEG antibodies. |
| doi_str_mv | 10.4155/bio.15.112 |
| format | Article |
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Antibodies reactive to PEG were engineered with a human IgG1 Fc. Surface plasmon resonance and plate-based methods demonstrated that their binding was dependent on molecular weight (MW) of PEG. Specificity experiments using chemical analogs identified their specificity.
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Antibodies reactive to PEG were engineered with a human IgG1 Fc. Surface plasmon resonance and plate-based methods demonstrated that their binding was dependent on molecular weight (MW) of PEG. Specificity experiments using chemical analogs identified their specificity.
Affinity, specificity and MW of PEG are critical characteristics that impact interactions of anti-PEG antibodies with PEG. These attributes especially MW of PEG and the assay formats may impact the ability to detect anti-PEG antibodies.</description><subject>Animals</subject><subject>Antibodies, Anti-Idiotypic - chemistry</subject><subject>Antibody Formation</subject><subject>Humans</subject><subject>Mice</subject><subject>Polyethylene Glycols - chemistry</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EolXphg9AXiMl2E7t2EtU2lCpEixgHflZDEkc2QGpfD2B0s7mjmaO7uIAcI1RvsCU3ikfckxzjMkZmOKSlhnDQpyfdo4mYJ7SOxqnIFwsxCWYEEYEFZxPwceD_bJN6FvbDVB2Buo3GaUebPTfcvChg8GN98GrYPYwWrkbwQSHAGVKNqW_X_a8quBmVx1BbxP0HdSN77yWDUyy7RubrsCFk02y8_-cgdf16mX5mG2fqs3yfptpwsshw5KXymnujOBCYGxKXTqlVCEIcwYhqrm1iDNOGMWIGsUUZVoWSCOkmEbFDNweenUMKUXr6j76VsZ9jVH9K60epdWY1qO0Eb45wP2naq05oUdFxQ_-UWkz</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Krishna, Murli</creator><creator>Palme, Holly</creator><creator>Duo, Jia</creator><creator>Lin, Zheng</creator><creator>Corbett, Martin</creator><creator>Dodge, Robert</creator><creator>Piccoli, Steven</creator><creator>Myler, Heather</creator><creator>Pillutla, Renuka</creator><creator>Desilva, Binodh</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20150101</creationdate><title>Development and characterization of antibody reagents to assess anti-PEG IgG antibodies in clinical samples</title><author>Krishna, Murli ; Palme, Holly ; Duo, Jia ; Lin, Zheng ; Corbett, Martin ; Dodge, Robert ; Piccoli, Steven ; Myler, Heather ; Pillutla, Renuka ; Desilva, Binodh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c287t-1a87bfc8fd989911d7c7fbbb3926fd005c8ee0868265105db6b56ca30c00b6c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antibodies, Anti-Idiotypic - chemistry</topic><topic>Antibody Formation</topic><topic>Humans</topic><topic>Mice</topic><topic>Polyethylene Glycols - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krishna, Murli</creatorcontrib><creatorcontrib>Palme, Holly</creatorcontrib><creatorcontrib>Duo, Jia</creatorcontrib><creatorcontrib>Lin, Zheng</creatorcontrib><creatorcontrib>Corbett, Martin</creatorcontrib><creatorcontrib>Dodge, Robert</creatorcontrib><creatorcontrib>Piccoli, Steven</creatorcontrib><creatorcontrib>Myler, Heather</creatorcontrib><creatorcontrib>Pillutla, Renuka</creatorcontrib><creatorcontrib>Desilva, Binodh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krishna, Murli</au><au>Palme, Holly</au><au>Duo, Jia</au><au>Lin, Zheng</au><au>Corbett, Martin</au><au>Dodge, Robert</au><au>Piccoli, Steven</au><au>Myler, Heather</au><au>Pillutla, Renuka</au><au>Desilva, Binodh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of antibody reagents to assess anti-PEG IgG antibodies in clinical samples</atitle><jtitle>Bioanalysis</jtitle><addtitle>Bioanalysis</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>7</volume><issue>15</issue><spage>1869</spage><epage>1883</epage><pages>1869-1883</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>Polyethylene glycol (PEG) is a polymer that can be conjugated with therapeutic proteins. Monitoring anti-PEG antibodies in human subjects may be required as part of immunogenicity assessment. The lack of well-characterized anti-PEG reagents have limited our understanding of anti-PEG humoral response.
Antibodies reactive to PEG were engineered with a human IgG1 Fc. Surface plasmon resonance and plate-based methods demonstrated that their binding was dependent on molecular weight (MW) of PEG. Specificity experiments using chemical analogs identified their specificity.
Affinity, specificity and MW of PEG are critical characteristics that impact interactions of anti-PEG antibodies with PEG. These attributes especially MW of PEG and the assay formats may impact the ability to detect anti-PEG antibodies.</abstract><cop>England</cop><pmid>26295988</pmid><doi>10.4155/bio.15.112</doi><tpages>15</tpages></addata></record> |
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| ispartof | Bioanalysis, 2015-01, Vol.7 (15), p.1869-1883 |
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| source | MEDLINE; PubMed Central |
| subjects | Animals Antibodies, Anti-Idiotypic - chemistry Antibody Formation Humans Mice Polyethylene Glycols - chemistry |
| title | Development and characterization of antibody reagents to assess anti-PEG IgG antibodies in clinical samples |
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