Alternative strategies for mass spectrometer-based sample dilution of bioanalytical samples, with particular reference to DBS and plasma analysis
The analysis of bioanalytical samples has required a physical dilution of high-concentration samples to bring concentrations into the validated calibration range of an assay. A reversed phase ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantitative analysis o...
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| Veröffentlicht in: | Bioanalysis 2014-03, Vol.6 (6), p.773-784 |
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| container_title | Bioanalysis |
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| creator | Mannu, Ranbir S Turpin, Phillip E Goodwin, Lee |
| description | The analysis of bioanalytical samples has required a physical dilution of high-concentration samples to bring concentrations into the validated calibration range of an assay.
A reversed phase ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantitative analysis of pioglitazone in dried blood spots has been used to partially validate two novel techniques to analyze sample concentrations that lie above a particular calibration range. The first of the two techniques is mass spectrometer signal dilution, which consists of lowering the signal that reaches the detector. The second technique designated isotope signal ratio monitoring looks at [M+2]+1 ions (caused by naturally occurring isotopes) for samples above the upper limit of quantification.
The newly developed methods have the potential to simplify the analysis of bioanalytical samples for which previously a physical dilution of the sample was required to bring analytes within the calibration range of an assay. |
| doi_str_mv | 10.4155/bio.13.320 |
| format | Article |
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A reversed phase ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantitative analysis of pioglitazone in dried blood spots has been used to partially validate two novel techniques to analyze sample concentrations that lie above a particular calibration range. The first of the two techniques is mass spectrometer signal dilution, which consists of lowering the signal that reaches the detector. The second technique designated isotope signal ratio monitoring looks at [M+2]+1 ions (caused by naturally occurring isotopes) for samples above the upper limit of quantification.
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A reversed phase ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantitative analysis of pioglitazone in dried blood spots has been used to partially validate two novel techniques to analyze sample concentrations that lie above a particular calibration range. The first of the two techniques is mass spectrometer signal dilution, which consists of lowering the signal that reaches the detector. The second technique designated isotope signal ratio monitoring looks at [M+2]+1 ions (caused by naturally occurring isotopes) for samples above the upper limit of quantification.
The newly developed methods have the potential to simplify the analysis of bioanalytical samples for which previously a physical dilution of the sample was required to bring analytes within the calibration range of an assay.</description><subject>Biological Assay - methods</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Dried Blood Spot Testing</subject><subject>Humans</subject><subject>Mass Spectrometry - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1757-6180</issn><issn>1757-6199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOAzEMRSMEolXphg9AWSOmJJN5ZVnKU6rEAliPnBkHgjIPJSmon8EfE2jBG1tXx9b1JeSUs0XG8_xSmWHBxUKk7IBMeZmXScGlPPyfKzYhc-_fWSyRVjKTx2SSZiVLOedT8rW0AV0PwXwg9cFBwFeDnurB0Q68p37EJrihw4glCjy21EM3WqStsZtghp4OmkYX0IPdBtOA3QP-gn6a8EZHcFHeWHDUoUaHfYM0DPT66olC39LRgu-A_u5740_IkQbrcb7vM_Jye_O8uk_Wj3cPq-U6aVKWhqRstJIKZM6BKcG5klWjUPNCZxoKLHVbCS10VWKFuc5yZLJsq0JyJRgrUiVm5Hx3t3GD99FZPTrTgdvWnNU_0dbxqZqLOkYb4bMdPG5Uh-0_-hek-AY2oHh6</recordid><startdate>201403</startdate><enddate>201403</enddate><creator>Mannu, Ranbir S</creator><creator>Turpin, Phillip E</creator><creator>Goodwin, Lee</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201403</creationdate><title>Alternative strategies for mass spectrometer-based sample dilution of bioanalytical samples, with particular reference to DBS and plasma analysis</title><author>Mannu, Ranbir S ; Turpin, Phillip E ; Goodwin, Lee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c202t-7cfb9ba951a0b311b98cbef16f4fa6e7fd83f3f87e8e5f45e097d8691b30062b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological Assay - methods</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Dried Blood Spot Testing</topic><topic>Humans</topic><topic>Mass Spectrometry - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mannu, Ranbir S</creatorcontrib><creatorcontrib>Turpin, Phillip E</creatorcontrib><creatorcontrib>Goodwin, Lee</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioanalysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mannu, Ranbir S</au><au>Turpin, Phillip E</au><au>Goodwin, Lee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative strategies for mass spectrometer-based sample dilution of bioanalytical samples, with particular reference to DBS and plasma analysis</atitle><jtitle>Bioanalysis</jtitle><addtitle>Bioanalysis</addtitle><date>2014-03</date><risdate>2014</risdate><volume>6</volume><issue>6</issue><spage>773</spage><epage>784</epage><pages>773-784</pages><issn>1757-6180</issn><eissn>1757-6199</eissn><abstract>The analysis of bioanalytical samples has required a physical dilution of high-concentration samples to bring concentrations into the validated calibration range of an assay.
A reversed phase ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantitative analysis of pioglitazone in dried blood spots has been used to partially validate two novel techniques to analyze sample concentrations that lie above a particular calibration range. The first of the two techniques is mass spectrometer signal dilution, which consists of lowering the signal that reaches the detector. The second technique designated isotope signal ratio monitoring looks at [M+2]+1 ions (caused by naturally occurring isotopes) for samples above the upper limit of quantification.
The newly developed methods have the potential to simplify the analysis of bioanalytical samples for which previously a physical dilution of the sample was required to bring analytes within the calibration range of an assay.</abstract><cop>England</cop><pmid>24702111</pmid><doi>10.4155/bio.13.320</doi><tpages>12</tpages></addata></record> |
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| ispartof | Bioanalysis, 2014-03, Vol.6 (6), p.773-784 |
| issn | 1757-6180 1757-6199 |
| language | eng |
| recordid | cdi_crossref_primary_10_4155_bio_13_320 |
| source | MEDLINE; PubMed Central |
| subjects | Biological Assay - methods Chromatography, High Pressure Liquid - methods Dried Blood Spot Testing Humans Mass Spectrometry - methods Tandem Mass Spectrometry - methods |
| title | Alternative strategies for mass spectrometer-based sample dilution of bioanalytical samples, with particular reference to DBS and plasma analysis |
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