Antioxidant, anti-amylase and lipid lowering potential of leaves of Aporosa lindleyana Baill. (Kebella)

Antioxidant, anti-amylase and lipid lowering potential of leaves of Aporosa lindleyana Baill. (Kebella)

Globally, the demand for herbal products with antioxidant capacity is increased due to many health benefits. The approach to discover new drugs through natural products has become a successful strategy in recent years. Aporosa lindleyana Baill. (Kebella) is used as a leafy vegetable in Sri Lanka. Al...

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Veröffentlicht in:Sri Lankan journal of biology 2018-01, Vol.3 (1), p.1-10
Hauptverfasser: Kathirgamanathar, S., Abeysekera, W. P. K. M., Weerasinghe, D. M. K. P., Ranasinghe, P., Binduhewa, A. M. C. U.
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container_issue 1
container_start_page 1
container_title Sri Lankan journal of biology
container_volume 3
creator Kathirgamanathar, S.
Abeysekera, W. P. K. M.
Weerasinghe, D. M. K. P.
Ranasinghe, P.
Binduhewa, A. M. C. U.
description Globally, the demand for herbal products with antioxidant capacity is increased due to many health benefits. The approach to discover new drugs through natural products has become a successful strategy in recent years. Aporosa lindleyana Baill. (Kebella) is used as a leafy vegetable in Sri Lanka. Although the root and bark of this plant showed many biological activities, only antioxidant activity by DPPH mechanism and anti-inflammatory activity are reported in leaf. The present study investigated the antioxidants [Total Phenolic Content (TPC) and Total Flavonoid Content (TFC)], antioxidant activities [Ferric Reducing Antioxidant Power (FRAP),1,1-diphenyl-2-picrylhydrazyl radicalscavenging assay (DPPH),2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging assay (ABTS) and Oxygen Radical Absorbance Capacity (ORAC)], anti-amylase activity(using acarbosestandard)and anti-lipidaemicactivities via anti-lipase (AL), anti-cholesterolesterase (AC) and bile acids binding (BAB) invitro of 95% ethanolic extract (EE) and water extract (WE) of A.lindleyanaleaves along with its nutritional value.Both extracts showed significant differences (P
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(Kebella)</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Kathirgamanathar, S. ; Abeysekera, W. P. K. M. ; Weerasinghe, D. M. K. P. ; Ranasinghe, P. ; Binduhewa, A. M. C. U.</creator><creatorcontrib>Kathirgamanathar, S. ; Abeysekera, W. P. K. M. ; Weerasinghe, D. M. K. P. ; Ranasinghe, P. ; Binduhewa, A. M. C. U.</creatorcontrib><description>Globally, the demand for herbal products with antioxidant capacity is increased due to many health benefits. The approach to discover new drugs through natural products has become a successful strategy in recent years. Aporosa lindleyana Baill. (Kebella) is used as a leafy vegetable in Sri Lanka. Although the root and bark of this plant showed many biological activities, only antioxidant activity by DPPH mechanism and anti-inflammatory activity are reported in leaf. The present study investigated the antioxidants [Total Phenolic Content (TPC) and Total Flavonoid Content (TFC)], antioxidant activities [Ferric Reducing Antioxidant Power (FRAP),1,1-diphenyl-2-picrylhydrazyl radicalscavenging assay (DPPH),2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging assay (ABTS) and Oxygen Radical Absorbance Capacity (ORAC)], anti-amylase activity(using acarbosestandard)and anti-lipidaemicactivities via anti-lipase (AL), anti-cholesterolesterase (AC) and bile acids binding (BAB) invitro of 95% ethanolic extract (EE) and water extract (WE) of A.lindleyanaleaves along with its nutritional value.Both extracts showed significant differences (P&lt;0.05) among the investigated antioxidants and antioxidant activities. For all the studied antioxidant activities, the EE showed high activity compared to WE and lower antioxidants in TPC. EE showed higher anti-amylase activity (IC50:164.85±8.36 μg/mL) compared to WE (IC50: 746.25±53.64 μg/mL). The IC50 of the standard drug, a carbose is 133.88 ± 4.4 μg/mL. Both extracts had dose dependant anti-lipidaemic activities in terms of anti-lipase, anti-cholesterol esterase and bile acids binding. However, EE showed significantly higher(p&lt;0.05)anti-lipase and anti-cholesterol esterase activities compared to WE. In contrast, WE showed significantly higher (p&lt;0.05) BAB for studied bile acids compared to EE (excepttaurocholate binding). Both extracts showed high taurocholate binding,similar binding of glycodeoxycholate and chenodeoxycholate and moderate anti-lipase activity compared to the reference drug (orlistat) studied.The proximate composition: moisture, carbohydrate, protein, crude fat, crude fibre and ash were 12.2, 49.4, 13.2, 1.1, 16.0 and 8.1%respectively. The minerals (Na, Mn, Fe, P, Zn, Cu: 53.7, 49.3, 41.4, 27.6, 22.6 and 10.4mg/kg; K, Ca and Mg: 18, 3.2and 2.7 g/kg, respectively) were also present. The leavesof A. lindleyana possess marked antioxidant, anti-amylase and moderate lipid lowering activities. 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M.</creatorcontrib><creatorcontrib>Weerasinghe, D. M. K. P.</creatorcontrib><creatorcontrib>Ranasinghe, P.</creatorcontrib><creatorcontrib>Binduhewa, A. M. C. U.</creatorcontrib><title>Antioxidant, anti-amylase and lipid lowering potential of leaves of Aporosa lindleyana Baill. (Kebella)</title><title>Sri Lankan journal of biology</title><description>Globally, the demand for herbal products with antioxidant capacity is increased due to many health benefits. The approach to discover new drugs through natural products has become a successful strategy in recent years. Aporosa lindleyana Baill. (Kebella) is used as a leafy vegetable in Sri Lanka. Although the root and bark of this plant showed many biological activities, only antioxidant activity by DPPH mechanism and anti-inflammatory activity are reported in leaf. The present study investigated the antioxidants [Total Phenolic Content (TPC) and Total Flavonoid Content (TFC)], antioxidant activities [Ferric Reducing Antioxidant Power (FRAP),1,1-diphenyl-2-picrylhydrazyl radicalscavenging assay (DPPH),2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging assay (ABTS) and Oxygen Radical Absorbance Capacity (ORAC)], anti-amylase activity(using acarbosestandard)and anti-lipidaemicactivities via anti-lipase (AL), anti-cholesterolesterase (AC) and bile acids binding (BAB) invitro of 95% ethanolic extract (EE) and water extract (WE) of A.lindleyanaleaves along with its nutritional value.Both extracts showed significant differences (P&lt;0.05) among the investigated antioxidants and antioxidant activities. For all the studied antioxidant activities, the EE showed high activity compared to WE and lower antioxidants in TPC. EE showed higher anti-amylase activity (IC50:164.85±8.36 μg/mL) compared to WE (IC50: 746.25±53.64 μg/mL). The IC50 of the standard drug, a carbose is 133.88 ± 4.4 μg/mL. Both extracts had dose dependant anti-lipidaemic activities in terms of anti-lipase, anti-cholesterol esterase and bile acids binding. However, EE showed significantly higher(p&lt;0.05)anti-lipase and anti-cholesterol esterase activities compared to WE. In contrast, WE showed significantly higher (p&lt;0.05) BAB for studied bile acids compared to EE (excepttaurocholate binding). Both extracts showed high taurocholate binding,similar binding of glycodeoxycholate and chenodeoxycholate and moderate anti-lipase activity compared to the reference drug (orlistat) studied.The proximate composition: moisture, carbohydrate, protein, crude fat, crude fibre and ash were 12.2, 49.4, 13.2, 1.1, 16.0 and 8.1%respectively. The minerals (Na, Mn, Fe, P, Zn, Cu: 53.7, 49.3, 41.4, 27.6, 22.6 and 10.4mg/kg; K, Ca and Mg: 18, 3.2and 2.7 g/kg, respectively) were also present. The leavesof A. lindleyana possess marked antioxidant, anti-amylase and moderate lipid lowering activities. 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P.</creatorcontrib><creatorcontrib>Ranasinghe, P.</creatorcontrib><creatorcontrib>Binduhewa, A. M. C. U.</creatorcontrib><collection>CrossRef</collection><jtitle>Sri Lankan journal of biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kathirgamanathar, S.</au><au>Abeysekera, W. P. K. M.</au><au>Weerasinghe, D. M. K. P.</au><au>Ranasinghe, P.</au><au>Binduhewa, A. M. C. U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant, anti-amylase and lipid lowering potential of leaves of Aporosa lindleyana Baill. (Kebella)</atitle><jtitle>Sri Lankan journal of biology</jtitle><date>2018-01-30</date><risdate>2018</risdate><volume>3</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>2513-2245</issn><eissn>2550-3340</eissn><abstract>Globally, the demand for herbal products with antioxidant capacity is increased due to many health benefits. The approach to discover new drugs through natural products has become a successful strategy in recent years. Aporosa lindleyana Baill. (Kebella) is used as a leafy vegetable in Sri Lanka. Although the root and bark of this plant showed many biological activities, only antioxidant activity by DPPH mechanism and anti-inflammatory activity are reported in leaf. The present study investigated the antioxidants [Total Phenolic Content (TPC) and Total Flavonoid Content (TFC)], antioxidant activities [Ferric Reducing Antioxidant Power (FRAP),1,1-diphenyl-2-picrylhydrazyl radicalscavenging assay (DPPH),2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging assay (ABTS) and Oxygen Radical Absorbance Capacity (ORAC)], anti-amylase activity(using acarbosestandard)and anti-lipidaemicactivities via anti-lipase (AL), anti-cholesterolesterase (AC) and bile acids binding (BAB) invitro of 95% ethanolic extract (EE) and water extract (WE) of A.lindleyanaleaves along with its nutritional value.Both extracts showed significant differences (P&lt;0.05) among the investigated antioxidants and antioxidant activities. For all the studied antioxidant activities, the EE showed high activity compared to WE and lower antioxidants in TPC. EE showed higher anti-amylase activity (IC50:164.85±8.36 μg/mL) compared to WE (IC50: 746.25±53.64 μg/mL). The IC50 of the standard drug, a carbose is 133.88 ± 4.4 μg/mL. Both extracts had dose dependant anti-lipidaemic activities in terms of anti-lipase, anti-cholesterol esterase and bile acids binding. However, EE showed significantly higher(p&lt;0.05)anti-lipase and anti-cholesterol esterase activities compared to WE. In contrast, WE showed significantly higher (p&lt;0.05) BAB for studied bile acids compared to EE (excepttaurocholate binding). Both extracts showed high taurocholate binding,similar binding of glycodeoxycholate and chenodeoxycholate and moderate anti-lipase activity compared to the reference drug (orlistat) studied.The proximate composition: moisture, carbohydrate, protein, crude fat, crude fibre and ash were 12.2, 49.4, 13.2, 1.1, 16.0 and 8.1%respectively. The minerals (Na, Mn, Fe, P, Zn, Cu: 53.7, 49.3, 41.4, 27.6, 22.6 and 10.4mg/kg; K, Ca and Mg: 18, 3.2and 2.7 g/kg, respectively) were also present. The leavesof A. lindleyana possess marked antioxidant, anti-amylase and moderate lipid lowering activities. This is the first report on multiple mechanisms of antioxidant activities, anti-amylase and lipid lowering effects which indicates the potential of developing A. lindleyana leaves as a functional food in prevention and management of non-communicable diseases.</abstract><doi>10.4038/sljb.v3i1.13</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0695-377X</orcidid><oa>free_for_read</oa></addata></record>
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title Antioxidant, anti-amylase and lipid lowering potential of leaves of Aporosa lindleyana Baill. (Kebella)
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