Study of neutrophil functions in patients on hemodialysis and the effects of rhG-CSF

The chemotaxis of neutrophils in peripheral blood was measured y the agarose plate method and their phagocytosis by the flow cytometry method in patients on hemodialysis. The in vitro effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on these neutrophil functions was also i...

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Veröffentlicht in:Journal of Japanese Society for Dialysis Therapy 1993/09/28, Vol.26(9), pp.1497-1502
Hauptverfasser: Kuroda, Yasuji, Kanatsu, Kazuro, Sekita, Kenichi, Takamitsu, Yoshihiro, Kamidono, Sadao
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container_issue 9
container_start_page 1497
container_title Journal of Japanese Society for Dialysis Therapy
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creator Kuroda, Yasuji
Kanatsu, Kazuro
Sekita, Kenichi
Takamitsu, Yoshihiro
Kamidono, Sadao
description The chemotaxis of neutrophils in peripheral blood was measured y the agarose plate method and their phagocytosis by the flow cytometry method in patients on hemodialysis. The in vitro effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on these neutrophil functions was also investigated. In patients on hemodialysis, both the chemotaxis and phagocytosis of neutrophils were reduced as compared to those of healthy individuals, and defense capability against infection was significantly reduced (p
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The in vitro effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) on these neutrophil functions was also investigated. In patients on hemodialysis, both the chemotaxis and phagocytosis of neutrophils were reduced as compared to those of healthy individuals, and defense capability against infection was significantly reduced (p&lt;0.01). Incubation with 1ng/ml of rhG-CSH caused significant enhancement of neutrophil function (chemotaxis and phagocytosis) in human dialysis patients (p&lt;0.01). However, no further increases in neutrophil function were seen at concentrations of rhG-CSF higher than 1ng/ml. 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title Study of neutrophil functions in patients on hemodialysis and the effects of rhG-CSF
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