VEGF-A not Ang2 mediates endothelial-like differentiation of immature DCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma
Endothelial-like differentiation of dendritic cells (DCs) is an interesting and significant phenomenon, which is worth further investigation. Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue h...
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| Veröffentlicht in: | International journal of oncology 2011-06, Vol.38 (6), p.1579-1588 |
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| container_title | International journal of oncology |
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| creator | Lu, Jing Liu, Kangdong Zhao, Jimin Zhao, Jun Ma, Junfen Yang, Hongyan Huang, Youtian Qin, Zhenzhu Bai, Ruihua Jiang, Lili Lv, Fengshou Li, Pei Yan, Wenhai Zhao, Mingyao Dong, Ziming |
| description | Endothelial-like differentiation of dendritic cells (DCs) is an interesting and significant phenomenon, which is worth further investigation. Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue homogenate can promote immature DCs (iDCs) to differentiate from the DC pathway toward endothelial cells, while the peri-carcinoma homogenate supernatant does not have this role. Inhibition of angiopoietin-2 (Ang2) in the supernatant by its antibody has no obvious influence on the endothelial-like differentiation. In contrast, inhibition of vascular endothelial growth factor-A (VEGF-A) blocked the differentiation. During the course of differentiation, a sustained activation of ERK1/2 was detected. PD98059 blocked the phosphorylation of ERK1/2 as well as the endothelial-like differentiation of iDCs. Inhibition of VEGF-A also blocked the phosphorylation of ERK1/2. These data suggest that VEGF-A not Ang2 mediates endothelial-like differentiation of iDCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma. |
| doi_str_mv | 10.3892/ijo.2011.989 |
| format | Article |
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Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue homogenate can promote immature DCs (iDCs) to differentiate from the DC pathway toward endothelial cells, while the peri-carcinoma homogenate supernatant does not have this role. Inhibition of angiopoietin-2 (Ang2) in the supernatant by its antibody has no obvious influence on the endothelial-like differentiation. In contrast, inhibition of vascular endothelial growth factor-A (VEGF-A) blocked the differentiation. During the course of differentiation, a sustained activation of ERK1/2 was detected. PD98059 blocked the phosphorylation of ERK1/2 as well as the endothelial-like differentiation of iDCs. Inhibition of VEGF-A also blocked the phosphorylation of ERK1/2. These data suggest that VEGF-A not Ang2 mediates endothelial-like differentiation of iDCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.2011.989</identifier><identifier>PMID: 21455573</identifier><language>eng</language><publisher>Athens: Editorial Academy of the International Journal of Oncology</publisher><subject>Adenocarcinoma - physiopathology ; Angiopoietin-2 - metabolism ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cells, Cultured ; Colonic Neoplasms - physiopathology ; Culture Media, Conditioned ; Dendritic Cells - cytology ; Dendritic Cells - drug effects ; Dendritic Cells - metabolism ; Endothelial Cells - metabolism ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Flavonoids - pharmacology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Medical sciences ; Protein Kinase Inhibitors - pharmacology ; Signal Transduction - drug effects ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tumor Microenvironment ; Tumors ; Vascular Endothelial Growth Factor A - antagonists & inhibitors ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>International journal of oncology, 2011-06, Vol.38 (6), p.1579-1588</ispartof><rights>2015 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-3da32f580cafb42e0d94a72211fb80743a85daa81498c8ea50461664b646b8ee3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24137314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21455573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Jing</creatorcontrib><creatorcontrib>Liu, Kangdong</creatorcontrib><creatorcontrib>Zhao, Jimin</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Ma, Junfen</creatorcontrib><creatorcontrib>Yang, Hongyan</creatorcontrib><creatorcontrib>Huang, Youtian</creatorcontrib><creatorcontrib>Qin, Zhenzhu</creatorcontrib><creatorcontrib>Bai, Ruihua</creatorcontrib><creatorcontrib>Jiang, Lili</creatorcontrib><creatorcontrib>Lv, Fengshou</creatorcontrib><creatorcontrib>Li, Pei</creatorcontrib><creatorcontrib>Yan, Wenhai</creatorcontrib><creatorcontrib>Zhao, Mingyao</creatorcontrib><creatorcontrib>Dong, Ziming</creatorcontrib><title>VEGF-A not Ang2 mediates endothelial-like differentiation of immature DCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>Endothelial-like differentiation of dendritic cells (DCs) is an interesting and significant phenomenon, which is worth further investigation. Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue homogenate can promote immature DCs (iDCs) to differentiate from the DC pathway toward endothelial cells, while the peri-carcinoma homogenate supernatant does not have this role. Inhibition of angiopoietin-2 (Ang2) in the supernatant by its antibody has no obvious influence on the endothelial-like differentiation. In contrast, inhibition of vascular endothelial growth factor-A (VEGF-A) blocked the differentiation. During the course of differentiation, a sustained activation of ERK1/2 was detected. PD98059 blocked the phosphorylation of ERK1/2 as well as the endothelial-like differentiation of iDCs. Inhibition of VEGF-A also blocked the phosphorylation of ERK1/2. These data suggest that VEGF-A not Ang2 mediates endothelial-like differentiation of iDCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma.</description><subject>Adenocarcinoma - physiopathology</subject><subject>Angiopoietin-2 - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Colonic Neoplasms - physiopathology</subject><subject>Culture Media, Conditioned</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - metabolism</subject><subject>Endothelial Cells - metabolism</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Flavonoids - pharmacology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Protein Kinase Inhibitors - pharmacology</subject><subject>Signal Transduction - drug effects</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Tumor Microenvironment</subject><subject>Tumors</subject><subject>Vascular Endothelial Growth Factor A - antagonists & inhibitors</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>1019-6439</issn><issn>1791-2423</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtKxDAUQIMovneuJRt3dsyzTZfDOD5wQBB1W27TZIy2iSQdwe_wh80wPla5kHMPl4PQCSUTrmp24V7DhBFKJ7Wqt9A-rWpaMMH4dp4JrYtS8HoPHaT0SgiTktBdtMeokFJWfB99Pc-vr4op9mHEU79keDCdg9EkbHwXxhfTO-iL3r0Z3DlrTTR-zP8ueBwsdsMA4yoafDlLuP3E84c7esFwcksPvfNL7DzODjw4HYPxHy4GP2TDevdlNYDHOvRZBZ3xQUPUzocBjtCOhT6Z45_3ED1dzR9nN8Xi_vp2Nl0Umks1FrwDzqxURINtBTOkqwVUjFFqW0UqwUHJDkBRUSutDEgiSlqWoi1F2Spj-CE633jzcSlFY5v36AaInw0lzbptk9s267ZNbpvx0w3-vmpzpT_4N2YGzn4ASBp6G8Frl_45QXnFqeDfLeyDLA</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Lu, Jing</creator><creator>Liu, Kangdong</creator><creator>Zhao, Jimin</creator><creator>Zhao, Jun</creator><creator>Ma, Junfen</creator><creator>Yang, Hongyan</creator><creator>Huang, Youtian</creator><creator>Qin, Zhenzhu</creator><creator>Bai, Ruihua</creator><creator>Jiang, Lili</creator><creator>Lv, Fengshou</creator><creator>Li, Pei</creator><creator>Yan, Wenhai</creator><creator>Zhao, Mingyao</creator><creator>Dong, Ziming</creator><general>Editorial Academy of the International Journal of Oncology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20110601</creationdate><title>VEGF-A not Ang2 mediates endothelial-like differentiation of immature DCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma</title><author>Lu, Jing ; Liu, Kangdong ; Zhao, Jimin ; Zhao, Jun ; Ma, Junfen ; Yang, Hongyan ; Huang, Youtian ; Qin, Zhenzhu ; Bai, Ruihua ; Jiang, Lili ; Lv, Fengshou ; Li, Pei ; Yan, Wenhai ; Zhao, Mingyao ; Dong, Ziming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-3da32f580cafb42e0d94a72211fb80743a85daa81498c8ea50461664b646b8ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenocarcinoma - physiopathology</topic><topic>Angiopoietin-2 - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Colonic Neoplasms - physiopathology</topic><topic>Culture Media, Conditioned</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - metabolism</topic><topic>Endothelial Cells - metabolism</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Flavonoids - pharmacology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Protein Kinase Inhibitors - pharmacology</topic><topic>Signal Transduction - drug effects</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. 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Here, we show that the tumor microenvironment derived from the supernatant of the SW620 human colon adenocarcinoma cell line and colon adenocarcinoma tissue homogenate can promote immature DCs (iDCs) to differentiate from the DC pathway toward endothelial cells, while the peri-carcinoma homogenate supernatant does not have this role. Inhibition of angiopoietin-2 (Ang2) in the supernatant by its antibody has no obvious influence on the endothelial-like differentiation. In contrast, inhibition of vascular endothelial growth factor-A (VEGF-A) blocked the differentiation. During the course of differentiation, a sustained activation of ERK1/2 was detected. PD98059 blocked the phosphorylation of ERK1/2 as well as the endothelial-like differentiation of iDCs. Inhibition of VEGF-A also blocked the phosphorylation of ERK1/2. These data suggest that VEGF-A not Ang2 mediates endothelial-like differentiation of iDCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma.</abstract><cop>Athens</cop><pub>Editorial Academy of the International Journal of Oncology</pub><pmid>21455573</pmid><doi>10.3892/ijo.2011.989</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1019-6439 |
| ispartof | International journal of oncology, 2011-06, Vol.38 (6), p.1579-1588 |
| issn | 1019-6439 1791-2423 |
| language | eng |
| recordid | cdi_crossref_primary_10_3892_ijo_2011_989 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenocarcinoma - physiopathology Angiopoietin-2 - metabolism Biological and medical sciences Cell Differentiation - drug effects Cells, Cultured Colonic Neoplasms - physiopathology Culture Media, Conditioned Dendritic Cells - cytology Dendritic Cells - drug effects Dendritic Cells - metabolism Endothelial Cells - metabolism Extracellular Signal-Regulated MAP Kinases - metabolism Flavonoids - pharmacology Gastroenterology. Liver. Pancreas. Abdomen Humans Medical sciences Protein Kinase Inhibitors - pharmacology Signal Transduction - drug effects Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumor Microenvironment Tumors Vascular Endothelial Growth Factor A - antagonists & inhibitors Vascular Endothelial Growth Factor A - metabolism |
| title | VEGF-A not Ang2 mediates endothelial-like differentiation of immature DCs by ERK1/2 signaling in the microenvironment of human colon adenocarcinoma |
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