MicroRNA profiling in murine liver after partial hepatectomy
Liver is uniquely capable to repair itself after injury. Multiple molecular and biochemical processes initiated after partial hepatectomy, lead to proliferation of all cells within the liver. MicroRNAs (miRNAs) are a class of highly abundant non-coding RNA molecules that cause post-transcriptional g...
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| Veröffentlicht in: | International journal of molecular medicine 2012-05, Vol.29 (5), p.747-755 |
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| container_title | International journal of molecular medicine |
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| creator | Chaveles, Ioannis Zaravinos, Apostolos Habeos, Ioannis Karavias, Dimitrios Maroulis, Ioannis Spandidos, Demetrios Karavias, Dionisios |
| description | Liver is uniquely capable to repair itself after injury. Multiple molecular
and biochemical processes initiated after partial hepatectomy, lead to proliferation
of all cells within the liver. MicroRNAs (miRNAs) are a class of highly abundant
non-coding RNA molecules that cause post-transcriptional gene repression and are
involved in several biological processes including cell cycle regulation and differentiation.
In this study, we examined the expression levels of miRNAs in liver tissue received
from control mice (L0) and compared them with the corresponding levels in liver
tissue 12 h after liver regeneration induced by 2/3 partial hepatectomy (L12).
MiRNA expression was investigated using microRNA profiling. Further qPCR analysis
was used for validation of the differentially expressed miRNAs at an early stage
of liver regeneration, induced by 2/3 partial hepatectomy. TargetScan and Gene
Ontology (GO) analyses were performed in order to identify the possible miRNA
target genes and their ontology, respectively. A subset of miRNAs was found to
be differentially expressed during liver regeneration. Mmu-miR-21 and mmu-miR-30b*
showed the higher levels of up-regulation in liver tissue from the hepatectomized
mice at the end of the experiment (L12) compared to the sham operated mice (L0).
Mmu-miR-21 up-regulation was further confirmed by qPCR. In situ hybridization
(ISH) revealed that mmu-miR-21 exhibited the higher levels of expression at 12
h post hepatectomy. On the contrary, mmu-miR-34c*, mmu-miR-144, mmu-miR-207, mmu-miR-207,
mmu-miR-451, mmu-miR-582-3p and mmu-miR-290-5p exhibited |
| doi_str_mv | 10.3892/ijmm.2012.902 |
| format | Article |
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and biochemical processes initiated after partial hepatectomy, lead to proliferation
of all cells within the liver. MicroRNAs (miRNAs) are a class of highly abundant
non-coding RNA molecules that cause post-transcriptional gene repression and are
involved in several biological processes including cell cycle regulation and differentiation.
In this study, we examined the expression levels of miRNAs in liver tissue received
from control mice (L0) and compared them with the corresponding levels in liver
tissue 12 h after liver regeneration induced by 2/3 partial hepatectomy (L12).
MiRNA expression was investigated using microRNA profiling. Further qPCR analysis
was used for validation of the differentially expressed miRNAs at an early stage
of liver regeneration, induced by 2/3 partial hepatectomy. TargetScan and Gene
Ontology (GO) analyses were performed in order to identify the possible miRNA
target genes and their ontology, respectively. A subset of miRNAs was found to
be differentially expressed during liver regeneration. Mmu-miR-21 and mmu-miR-30b*
showed the higher levels of up-regulation in liver tissue from the hepatectomized
mice at the end of the experiment (L12) compared to the sham operated mice (L0).
Mmu-miR-21 up-regulation was further confirmed by qPCR. In situ hybridization
(ISH) revealed that mmu-miR-21 exhibited the higher levels of expression at 12
h post hepatectomy. On the contrary, mmu-miR-34c*, mmu-miR-144, mmu-miR-207, mmu-miR-207,
mmu-miR-451, mmu-miR-582-3p and mmu-miR-290-5p exhibited <0.5 down-regulation
in liver tissue after partial hepatectomy in L12 vs. L0 mice. The results from
microarray and qPCR analyses were in good agreement. In conclusion, our results
provide important information regarding the differentially expressed miRNAs in
murine liver tissue before and after partial hepatectomy. The early up-regulation
of mmu-miR-21 during the process of liver regeneration suggests a regulatory role
in liver regeneration in vivo.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2012.902</identifier><identifier>PMID: 22307273</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Animals ; Gene Expression Profiling ; Gene Expression Regulation ; Hepatectomy ; Liver - metabolism ; Liver - physiology ; Liver Regeneration ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs - genetics</subject><ispartof>International journal of molecular medicine, 2012-05, Vol.29 (5), p.747-755</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-96aeab596e86ecda4bab812b02c295aba07c5ef28d00869fa8013bde741fbdad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,5558,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22307273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaveles, Ioannis</creatorcontrib><creatorcontrib>Zaravinos, Apostolos</creatorcontrib><creatorcontrib>Habeos, Ioannis</creatorcontrib><creatorcontrib>Karavias, Dimitrios</creatorcontrib><creatorcontrib>Maroulis, Ioannis</creatorcontrib><creatorcontrib>Spandidos, Demetrios</creatorcontrib><creatorcontrib>Karavias, Dionisios</creatorcontrib><title>MicroRNA profiling in murine liver after partial hepatectomy</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Liver is uniquely capable to repair itself after injury. Multiple molecular
and biochemical processes initiated after partial hepatectomy, lead to proliferation
of all cells within the liver. MicroRNAs (miRNAs) are a class of highly abundant
non-coding RNA molecules that cause post-transcriptional gene repression and are
involved in several biological processes including cell cycle regulation and differentiation.
In this study, we examined the expression levels of miRNAs in liver tissue received
from control mice (L0) and compared them with the corresponding levels in liver
tissue 12 h after liver regeneration induced by 2/3 partial hepatectomy (L12).
MiRNA expression was investigated using microRNA profiling. Further qPCR analysis
was used for validation of the differentially expressed miRNAs at an early stage
of liver regeneration, induced by 2/3 partial hepatectomy. TargetScan and Gene
Ontology (GO) analyses were performed in order to identify the possible miRNA
target genes and their ontology, respectively. A subset of miRNAs was found to
be differentially expressed during liver regeneration. Mmu-miR-21 and mmu-miR-30b*
showed the higher levels of up-regulation in liver tissue from the hepatectomized
mice at the end of the experiment (L12) compared to the sham operated mice (L0).
Mmu-miR-21 up-regulation was further confirmed by qPCR. In situ hybridization
(ISH) revealed that mmu-miR-21 exhibited the higher levels of expression at 12
h post hepatectomy. On the contrary, mmu-miR-34c*, mmu-miR-144, mmu-miR-207, mmu-miR-207,
mmu-miR-451, mmu-miR-582-3p and mmu-miR-290-5p exhibited <0.5 down-regulation
in liver tissue after partial hepatectomy in L12 vs. L0 mice. The results from
microarray and qPCR analyses were in good agreement. In conclusion, our results
provide important information regarding the differentially expressed miRNAs in
murine liver tissue before and after partial hepatectomy. The early up-regulation
of mmu-miR-21 during the process of liver regeneration suggests a regulatory role
in liver regeneration in vivo.</description><subject>Animals</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Hepatectomy</subject><subject>Liver - metabolism</subject><subject>Liver - physiology</subject><subject>Liver Regeneration</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>MicroRNAs - genetics</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEURoMotlaXbmX2MjWPySQBN6X4Ah8gCu6Gm0miKfMimQr996bU6ubeuzh8fPcgdE7wnElFr_yqbecUEzpXmB6gKRGK5LQoPg7TTbDImeDlBJ3EuMKY8kLJYzShlGFBBZui6ydfh_71eZENoXe-8d1n5rusXQff2azx3zZk4MY0Bwijhyb7sgOMth77dnOKjhw00Z797hl6v715W97njy93D8vFY16zUoy5KsGC5qq0srS1gUKDloRqTGuqOGjAoubWUWkwlqVyIDFh2lhREKcNGDZD-S43VY0xWFcNwbcQNhXB1dZCtbVQbS1UyULiL3b8sNatNX_0_u0EXO6AOEBnvOnjf-LeWaomCiE4Zz-Lemcz</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Chaveles, Ioannis</creator><creator>Zaravinos, Apostolos</creator><creator>Habeos, Ioannis</creator><creator>Karavias, Dimitrios</creator><creator>Maroulis, Ioannis</creator><creator>Spandidos, Demetrios</creator><creator>Karavias, Dionisios</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20120501</creationdate><title>MicroRNA profiling in murine liver after partial hepatectomy</title><author>Chaveles, Ioannis ; Zaravinos, Apostolos ; Habeos, Ioannis ; Karavias, Dimitrios ; Maroulis, Ioannis ; Spandidos, Demetrios ; Karavias, Dionisios</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-96aeab596e86ecda4bab812b02c295aba07c5ef28d00869fa8013bde741fbdad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Hepatectomy</topic><topic>Liver - metabolism</topic><topic>Liver - physiology</topic><topic>Liver Regeneration</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>MicroRNAs - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaveles, Ioannis</creatorcontrib><creatorcontrib>Zaravinos, Apostolos</creatorcontrib><creatorcontrib>Habeos, Ioannis</creatorcontrib><creatorcontrib>Karavias, Dimitrios</creatorcontrib><creatorcontrib>Maroulis, Ioannis</creatorcontrib><creatorcontrib>Spandidos, Demetrios</creatorcontrib><creatorcontrib>Karavias, Dionisios</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaveles, Ioannis</au><au>Zaravinos, Apostolos</au><au>Habeos, Ioannis</au><au>Karavias, Dimitrios</au><au>Maroulis, Ioannis</au><au>Spandidos, Demetrios</au><au>Karavias, Dionisios</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA profiling in murine liver after partial hepatectomy</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>29</volume><issue>5</issue><spage>747</spage><epage>755</epage><pages>747-755</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Liver is uniquely capable to repair itself after injury. Multiple molecular
and biochemical processes initiated after partial hepatectomy, lead to proliferation
of all cells within the liver. MicroRNAs (miRNAs) are a class of highly abundant
non-coding RNA molecules that cause post-transcriptional gene repression and are
involved in several biological processes including cell cycle regulation and differentiation.
In this study, we examined the expression levels of miRNAs in liver tissue received
from control mice (L0) and compared them with the corresponding levels in liver
tissue 12 h after liver regeneration induced by 2/3 partial hepatectomy (L12).
MiRNA expression was investigated using microRNA profiling. Further qPCR analysis
was used for validation of the differentially expressed miRNAs at an early stage
of liver regeneration, induced by 2/3 partial hepatectomy. TargetScan and Gene
Ontology (GO) analyses were performed in order to identify the possible miRNA
target genes and their ontology, respectively. A subset of miRNAs was found to
be differentially expressed during liver regeneration. Mmu-miR-21 and mmu-miR-30b*
showed the higher levels of up-regulation in liver tissue from the hepatectomized
mice at the end of the experiment (L12) compared to the sham operated mice (L0).
Mmu-miR-21 up-regulation was further confirmed by qPCR. In situ hybridization
(ISH) revealed that mmu-miR-21 exhibited the higher levels of expression at 12
h post hepatectomy. On the contrary, mmu-miR-34c*, mmu-miR-144, mmu-miR-207, mmu-miR-207,
mmu-miR-451, mmu-miR-582-3p and mmu-miR-290-5p exhibited <0.5 down-regulation
in liver tissue after partial hepatectomy in L12 vs. L0 mice. The results from
microarray and qPCR analyses were in good agreement. In conclusion, our results
provide important information regarding the differentially expressed miRNAs in
murine liver tissue before and after partial hepatectomy. The early up-regulation
of mmu-miR-21 during the process of liver regeneration suggests a regulatory role
in liver regeneration in vivo.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>22307273</pmid><doi>10.3892/ijmm.2012.902</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1107-3756 1791-244X |
| language | eng |
| recordid | cdi_crossref_primary_10_3892_ijmm_2012_902 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Gene Expression Profiling Gene Expression Regulation Hepatectomy Liver - metabolism Liver - physiology Liver Regeneration Male Mice Mice, Inbred C57BL MicroRNAs - genetics |
| title | MicroRNA profiling in murine liver after partial hepatectomy |
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