The Effect of hFVIII Transgene on the Chromosomal Aneuploidy Rate in Rabbits
The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits...
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| Veröffentlicht in: | Folia biologica (Kraków) 2007-01, Vol.55 (3), p.161-164 |
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| creator | Curlej, Jozef Parkanyi, Vladimir Bulla, Jozef Jurcik, Rastislav Chrenek, Peter |
| description | The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic
rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 μg/ml colcemide. No significant difference in chromosomal
aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P |
| doi_str_mv | 10.3409/173491607781492614 |
| format | Article |
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rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 μg/ml colcemide. No significant difference in chromosomal
aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate.
In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.</description><identifier>ISSN: 0015-5497</identifier><identifier>DOI: 10.3409/173491607781492614</identifier><identifier>PMID: 18274261</identifier><language>eng</language><publisher>Poland: Institute of Systematics and Evolution of Animals, Polish A</publisher><subject>Aneuploidy ; Animals ; Animals, Genetically Modified ; Base Sequence ; Bone Marrow Lymphocytes ; Chromosomal Aneuploidy ; DNA Primers ; Female ; Hfviii ; Humans ; Rabbit ; Rabbits ; Thromboplastin - genetics ; Transgene ; Transgenes</subject><ispartof>Folia biologica (Kraków), 2007-01, Vol.55 (3), p.161-164</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-2c152c76242658bfda4282a9132ad515b2be8c27d054a483455240ec62c208d93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>288,314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18274261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Curlej, Jozef</creatorcontrib><creatorcontrib>Parkanyi, Vladimir</creatorcontrib><creatorcontrib>Bulla, Jozef</creatorcontrib><creatorcontrib>Jurcik, Rastislav</creatorcontrib><creatorcontrib>Chrenek, Peter</creatorcontrib><title>The Effect of hFVIII Transgene on the Chromosomal Aneuploidy Rate in Rabbits</title><title>Folia biologica (Kraków)</title><addtitle>Folia Biol (Krakow)</addtitle><description>The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic
rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 μg/ml colcemide. No significant difference in chromosomal
aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate.
In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.</description><subject>Aneuploidy</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Base Sequence</subject><subject>Bone Marrow Lymphocytes</subject><subject>Chromosomal Aneuploidy</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Hfviii</subject><subject>Humans</subject><subject>Rabbit</subject><subject>Rabbits</subject><subject>Thromboplastin - genetics</subject><subject>Transgene</subject><subject>Transgenes</subject><issn>0015-5497</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1qGzEURrVoSdI0L9BF0ao7N1d_1mhVgklagyFQnGwvGo0UT5gZuZKmkDx95drQRag2H4hzD9J3CfnE4KuQYK6ZFtKwJWjdMGn4ksl35AKAqYWSRp-TDzk_AwgjQJ-Rc9ZwLSt0QTbbnae3IXhXaAx0d_e4Xq_pNtkpP_nJ0zjRUonVLsUx5jjagd5Mft4Pse9e6E9bPO2nmm3bl_yRvA92yP7qlJfk4e52u_qx2Nx_X69uNgsnuSkL7pjiTi95fYJq2tBZyRtuDRPcdoqplre-cVx3oKSVjZBKcQneLbnj0HRGXJIvR-8-xV-zzwXHPjs_DHbycc6ogTGozgryI-hSzDn5gPvUjza9IAM89IZve6tDn0_2uR1992_kVFoFvh2BfqodFYvPcU5T_TD22b9iaJEDaIS_RykMAPUCBNpUajJRDff_M7ij5LC6w-bwt1KTqELOwABHxpjAzgc7DwWLTfj0irka_wAea5Xb</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Curlej, Jozef</creator><creator>Parkanyi, Vladimir</creator><creator>Bulla, Jozef</creator><creator>Jurcik, Rastislav</creator><creator>Chrenek, Peter</creator><general>Institute of Systematics and Evolution of Animals, Polish A</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>The Effect of hFVIII Transgene on the Chromosomal Aneuploidy Rate in Rabbits</title><author>Curlej, Jozef ; Parkanyi, Vladimir ; Bulla, Jozef ; Jurcik, Rastislav ; Chrenek, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-2c152c76242658bfda4282a9132ad515b2be8c27d054a483455240ec62c208d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aneuploidy</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Base Sequence</topic><topic>Bone Marrow Lymphocytes</topic><topic>Chromosomal Aneuploidy</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Hfviii</topic><topic>Humans</topic><topic>Rabbit</topic><topic>Rabbits</topic><topic>Thromboplastin - genetics</topic><topic>Transgene</topic><topic>Transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Curlej, Jozef</creatorcontrib><creatorcontrib>Parkanyi, Vladimir</creatorcontrib><creatorcontrib>Bulla, Jozef</creatorcontrib><creatorcontrib>Jurcik, Rastislav</creatorcontrib><creatorcontrib>Chrenek, Peter</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Folia biologica (Kraków)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Curlej, Jozef</au><au>Parkanyi, Vladimir</au><au>Bulla, Jozef</au><au>Jurcik, Rastislav</au><au>Chrenek, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of hFVIII Transgene on the Chromosomal Aneuploidy Rate in Rabbits</atitle><jtitle>Folia biologica (Kraków)</jtitle><addtitle>Folia Biol (Krakow)</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>55</volume><issue>3</issue><spage>161</spage><epage>164</epage><pages>161-164</pages><issn>0015-5497</issn><abstract>The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic
rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 μg/ml colcemide. No significant difference in chromosomal
aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate.
In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.</abstract><cop>Poland</cop><pub>Institute of Systematics and Evolution of Animals, Polish A</pub><pmid>18274261</pmid><doi>10.3409/173491607781492614</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Aneuploidy Animals Animals, Genetically Modified Base Sequence Bone Marrow Lymphocytes Chromosomal Aneuploidy DNA Primers Female Hfviii Humans Rabbit Rabbits Thromboplastin - genetics Transgene Transgenes |
| title | The Effect of hFVIII Transgene on the Chromosomal Aneuploidy Rate in Rabbits |
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