Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets
To explore the relationship between intestinal mucin 2 ( ) and weaning-induced diarrhoea in piglets, we analysed and piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbe...
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| Veröffentlicht in: | International journal of molecular sciences 2025-01, Vol.26 (2), p.599 |
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| creator | Wang, Li Jin, Long Zhang, Liulian Huang, Xuankai Li, Ziyu Li, Zhimin Li, Ke Xu, Yuan Di, Shengwei Cui, Shiquan Wang, Xibiao |
| description | To explore the relationship between intestinal mucin 2 (
) and weaning-induced diarrhoea in piglets, we analysed
and
piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbers, sizes, and degrees of intestinal injury were observed and recorded. Intestinal tissue
mRNA and protein expression were analysed via quantitative real-time PCR (qRT-PCR) and Western blotting.
presented significantly lower diarrhoea rates and intestinal injury scores than
(
< 0.01). The intestinal injury scores in the weaned diarrhoea group were significantly greater than those in the unweaned groups (
< 0.05), with
consistently exhibiting lower injury scores than
. Specifically, unweaned
presented significantly greater duodenal
mRNA (
< 0.05), and weaned healthy
presented notably greater expression in both the duodenum and jejunum (
< 0.01). These findings reveal enhanced intestinal protection against weaning stress and diarrhoea in
, with elevated
levels likely contributing to lower injury scores and milder symptoms, thus highlighting the influence of genetic differences. |
| doi_str_mv | 10.3390/ijms26020599 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_crossref_primary_10_3390_ijms26020599</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3159504737</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1779-1312190f443430bdcb5514bd50aa400d9c18b58c3718a6ebfda2bd103c18567d3</originalsourceid><addsrcrecordid>eNpd0ctv3CAQB2AUtcpjm1vPEVIvPcTJAAabU9TmWWmjVnmoR4TN7C4bL96Cncd_H-epTU8g-DSamR8hXxnsCaFh388XiSvgILVeI5ss5zwDUMWnlfsG2UppDsAFl3qdbAhdSi2Y2iQXx_fLpo0-TGk3Qzr24Yb-xO4OMdDzvvaBcmqDo3_Rhid02UVMKbvAxnbo6JG3Mc5atHSQf_y0wS59IZ8ntkm4_XqOyPXJ8dXhWTb-ffrr8Mc4q1lR6IwJxpmGSZ6LXEDl6kpKlldOgrU5gNM1KytZ1qJgpVVYTZzllWMghnepCidG5OCl7rKvFuhqDF20jVlGv7DxwbTWm48_wc_MtL01jBVKqmEBI_L9tUJs__WYOrPwqcamsQHbPhnBpC6BcaUG-u0_Om_7GIb5npWEvBDFoHZfVB3blCJO3rthYJ7SMqtpDXxndYJ3_BaPeAT4JY9a</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3159504737</pqid></control><display><type>article</type><title>Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wang, Li ; Jin, Long ; Zhang, Liulian ; Huang, Xuankai ; Li, Ziyu ; Li, Zhimin ; Li, Ke ; Xu, Yuan ; Di, Shengwei ; Cui, Shiquan ; Wang, Xibiao</creator><creatorcontrib>Wang, Li ; Jin, Long ; Zhang, Liulian ; Huang, Xuankai ; Li, Ziyu ; Li, Zhimin ; Li, Ke ; Xu, Yuan ; Di, Shengwei ; Cui, Shiquan ; Wang, Xibiao</creatorcontrib><description>To explore the relationship between intestinal mucin 2 (
) and weaning-induced diarrhoea in piglets, we analysed
and
piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbers, sizes, and degrees of intestinal injury were observed and recorded. Intestinal tissue
mRNA and protein expression were analysed via quantitative real-time PCR (qRT-PCR) and Western blotting.
presented significantly lower diarrhoea rates and intestinal injury scores than
(
< 0.01). The intestinal injury scores in the weaned diarrhoea group were significantly greater than those in the unweaned groups (
< 0.05), with
consistently exhibiting lower injury scores than
. Specifically, unweaned
presented significantly greater duodenal
mRNA (
< 0.05), and weaned healthy
presented notably greater expression in both the duodenum and jejunum (
< 0.01). These findings reveal enhanced intestinal protection against weaning stress and diarrhoea in
, with elevated
levels likely contributing to lower injury scores and milder symptoms, thus highlighting the influence of genetic differences.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms26020599</identifier><identifier>PMID: 39859316</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Diarrhea ; Diarrhea - genetics ; Diarrhea - metabolism ; Goblet Cells - metabolism ; Goblet Cells - pathology ; Hogs ; Homeostasis ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Mucin-2 - genetics ; Mucin-2 - metabolism ; Protein expression ; Proteins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Stress, Physiological ; Swine ; Swine Diseases - metabolism ; Weaning</subject><ispartof>International journal of molecular sciences, 2025-01, Vol.26 (2), p.599</ispartof><rights>2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2025 by the authors. 2025</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1779-1312190f443430bdcb5514bd50aa400d9c18b58c3718a6ebfda2bd103c18567d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765659/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765659/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39859316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhang, Liulian</creatorcontrib><creatorcontrib>Huang, Xuankai</creatorcontrib><creatorcontrib>Li, Ziyu</creatorcontrib><creatorcontrib>Li, Zhimin</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Xu, Yuan</creatorcontrib><creatorcontrib>Di, Shengwei</creatorcontrib><creatorcontrib>Cui, Shiquan</creatorcontrib><creatorcontrib>Wang, Xibiao</creatorcontrib><title>Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>To explore the relationship between intestinal mucin 2 (
) and weaning-induced diarrhoea in piglets, we analysed
and
piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbers, sizes, and degrees of intestinal injury were observed and recorded. Intestinal tissue
mRNA and protein expression were analysed via quantitative real-time PCR (qRT-PCR) and Western blotting.
presented significantly lower diarrhoea rates and intestinal injury scores than
(
< 0.01). The intestinal injury scores in the weaned diarrhoea group were significantly greater than those in the unweaned groups (
< 0.05), with
consistently exhibiting lower injury scores than
. Specifically, unweaned
presented significantly greater duodenal
mRNA (
< 0.05), and weaned healthy
presented notably greater expression in both the duodenum and jejunum (
< 0.01). These findings reveal enhanced intestinal protection against weaning stress and diarrhoea in
, with elevated
levels likely contributing to lower injury scores and milder symptoms, thus highlighting the influence of genetic differences.</description><subject>Animals</subject><subject>Diarrhea</subject><subject>Diarrhea - genetics</subject><subject>Diarrhea - metabolism</subject><subject>Goblet Cells - metabolism</subject><subject>Goblet Cells - pathology</subject><subject>Hogs</subject><subject>Homeostasis</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Mucin-2 - genetics</subject><subject>Mucin-2 - metabolism</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Stress, Physiological</subject><subject>Swine</subject><subject>Swine Diseases - metabolism</subject><subject>Weaning</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpd0ctv3CAQB2AUtcpjm1vPEVIvPcTJAAabU9TmWWmjVnmoR4TN7C4bL96Cncd_H-epTU8g-DSamR8hXxnsCaFh388XiSvgILVeI5ss5zwDUMWnlfsG2UppDsAFl3qdbAhdSi2Y2iQXx_fLpo0-TGk3Qzr24Yb-xO4OMdDzvvaBcmqDo3_Rhid02UVMKbvAxnbo6JG3Mc5atHSQf_y0wS59IZ8ntkm4_XqOyPXJ8dXhWTb-ffrr8Mc4q1lR6IwJxpmGSZ6LXEDl6kpKlldOgrU5gNM1KytZ1qJgpVVYTZzllWMghnepCidG5OCl7rKvFuhqDF20jVlGv7DxwbTWm48_wc_MtL01jBVKqmEBI_L9tUJs__WYOrPwqcamsQHbPhnBpC6BcaUG-u0_Om_7GIb5npWEvBDFoHZfVB3blCJO3rthYJ7SMqtpDXxndYJ3_BaPeAT4JY9a</recordid><startdate>20250112</startdate><enddate>20250112</enddate><creator>Wang, Li</creator><creator>Jin, Long</creator><creator>Zhang, Liulian</creator><creator>Huang, Xuankai</creator><creator>Li, Ziyu</creator><creator>Li, Zhimin</creator><creator>Li, Ke</creator><creator>Xu, Yuan</creator><creator>Di, Shengwei</creator><creator>Cui, Shiquan</creator><creator>Wang, Xibiao</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20250112</creationdate><title>Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets</title><author>Wang, Li ; Jin, Long ; Zhang, Liulian ; Huang, Xuankai ; Li, Ziyu ; Li, Zhimin ; Li, Ke ; Xu, Yuan ; Di, Shengwei ; Cui, Shiquan ; Wang, Xibiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1779-1312190f443430bdcb5514bd50aa400d9c18b58c3718a6ebfda2bd103c18567d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Animals</topic><topic>Diarrhea</topic><topic>Diarrhea - genetics</topic><topic>Diarrhea - metabolism</topic><topic>Goblet Cells - metabolism</topic><topic>Goblet Cells - pathology</topic><topic>Hogs</topic><topic>Homeostasis</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Mucin-2 - genetics</topic><topic>Mucin-2 - metabolism</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Stress, Physiological</topic><topic>Swine</topic><topic>Swine Diseases - metabolism</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Jin, Long</creatorcontrib><creatorcontrib>Zhang, Liulian</creatorcontrib><creatorcontrib>Huang, Xuankai</creatorcontrib><creatorcontrib>Li, Ziyu</creatorcontrib><creatorcontrib>Li, Zhimin</creatorcontrib><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Xu, Yuan</creatorcontrib><creatorcontrib>Di, Shengwei</creatorcontrib><creatorcontrib>Cui, Shiquan</creatorcontrib><creatorcontrib>Wang, Xibiao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Li</au><au>Jin, Long</au><au>Zhang, Liulian</au><au>Huang, Xuankai</au><au>Li, Ziyu</au><au>Li, Zhimin</au><au>Li, Ke</au><au>Xu, Yuan</au><au>Di, Shengwei</au><au>Cui, Shiquan</au><au>Wang, Xibiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2025-01-12</date><risdate>2025</risdate><volume>26</volume><issue>2</issue><spage>599</spage><pages>599-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>To explore the relationship between intestinal mucin 2 (
) and weaning-induced diarrhoea in piglets, we analysed
and
piglets. The piglets were divided into a healthy weaned group, a weaned diarrhoea group, and a healthy unweaned control group. Intestinal tissues were collected, and goblet cell numbers, sizes, and degrees of intestinal injury were observed and recorded. Intestinal tissue
mRNA and protein expression were analysed via quantitative real-time PCR (qRT-PCR) and Western blotting.
presented significantly lower diarrhoea rates and intestinal injury scores than
(
< 0.01). The intestinal injury scores in the weaned diarrhoea group were significantly greater than those in the unweaned groups (
< 0.05), with
consistently exhibiting lower injury scores than
. Specifically, unweaned
presented significantly greater duodenal
mRNA (
< 0.05), and weaned healthy
presented notably greater expression in both the duodenum and jejunum (
< 0.01). These findings reveal enhanced intestinal protection against weaning stress and diarrhoea in
, with elevated
levels likely contributing to lower injury scores and milder symptoms, thus highlighting the influence of genetic differences.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39859316</pmid><doi>10.3390/ijms26020599</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of molecular sciences, 2025-01, Vol.26 (2), p.599 |
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| recordid | cdi_crossref_primary_10_3390_ijms26020599 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Diarrhea Diarrhea - genetics Diarrhea - metabolism Goblet Cells - metabolism Goblet Cells - pathology Hogs Homeostasis Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Mucin-2 - genetics Mucin-2 - metabolism Protein expression Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Stress, Physiological Swine Swine Diseases - metabolism Weaning |
| title | Exploring the Link Between Mucin 2 and Weaning Stress-Related Diarrhoea in Piglets |
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