BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function

BST204 is a purified ginseng dry extract that has an inhibitory effect on lipopolysaccharide-induced inflammatory responses, but its effect on muscle atrophy is yet to be investigated. In this study, C2C12 myoblasts were induced to differentiate for three days followed by the treatment of dexamethas...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of environmental research and public health 2021-03, Vol.18 (5), p.2367, Article 2367
Hauptverfasser: Kim, Ryuni, Kim, Hyebeen, Im, Minju, Park, Sun Kyu, Han, Hae Jung, An, Subin, Kang, Jong-Sun, Lee, Sang-Jin, Bae, Gyu-Un
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 5
container_start_page 2367
container_title International journal of environmental research and public health
container_volume 18
creator Kim, Ryuni
Kim, Hyebeen
Im, Minju
Park, Sun Kyu
Han, Hae Jung
An, Subin
Kang, Jong-Sun
Lee, Sang-Jin
Bae, Gyu-Un
description BST204 is a purified ginseng dry extract that has an inhibitory effect on lipopolysaccharide-induced inflammatory responses, but its effect on muscle atrophy is yet to be investigated. In this study, C2C12 myoblasts were induced to differentiate for three days followed by the treatment of dexamethasone (DEX), a corticosteroid drug, with vehicle or BST204 for one day and subjected to immunoblotting, immunocytochemistry, qRT-PCR and biochemical analysis for mitochondrial function. BST204 alleviates the myotube atrophic effect mediated by DEX via the activation of protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling. Through this pathway, BST204 suppresses the expression of muscle-specific E3 ubiquitin ligases contributing to the enhanced myotube formation and enlarged myotube diameter in DEX-treated myotubes. In addition, BST204 treatment significantly decreases the mitochondrial reactive oxygen species production in DEX-treated myotubes. Furthermore, BST204 improves mitochondrial function by upregulating the expression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC1 alpha) in DEX-induced myotube atrophy. This study provides a mechanistic insight into the effect of BST204 on DEX-induced myotube atrophy, suggesting that BST204 has protective effects against the toxicity of a corticosteroid drug in muscle and promising potential as a nutraceutical remedy for the treatment of muscle weakness and atrophy.
doi_str_mv 10.3390/ijerph18052367
format Article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3390_ijerph18052367</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>33804338</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-b176270e2604fb62147f39876072c3f15031e50bf0eedd736d6e9ef5d5143acf3</originalsourceid><addsrcrecordid>eNqNkdFP2zAQxq2JaRTY6x5R3lG6c5zYyQtS6eiGxLRJwHPkOOfGVWtHjgPrC387Zt0q-oYffNbd7_usuyPkC4UpYxV8NSv0fUdLKDLGxQcyoZxDmnOgR2_ex-RkGFYArMx59YkcM1ZCHq8Jeb66u88gT357F1CFIfmGf-QGQycHZzG9se2osE1-bl0YG0xmwbu-2yah825cdjFi8tB7XI5rGYyzidN7duH8ZpeUNjqY4FTnbOuNXCeL0arX0hn5qOV6wM__4il5WFzfz3-kt7--38xnt6mKTYa0oYJnAjDjkOuGZzQXmlWl4CAyxTQtgFEsoNGA2LaC8ZZjhbpoC5ozqTQ7JZc7335sNtgqtMHLdd17s5F-Wztp6sOKNV29dI-1qApR5BANpjsD5d0weNR7LYX6dRP14Sai4Pztj3v8_-gjUO6AJ2ycHpRBq3CPAQDPSlpEOh46N-HvKOdutCFKL94vZS-aeKmR</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Web of Science - Science Citation Index Expanded - 2021&lt;img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /&gt;</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Web of Science - Social Sciences Citation Index – 2021&lt;img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /&gt;</source><source>Free Full-Text Journals in Chemistry</source><creator>Kim, Ryuni ; Kim, Hyebeen ; Im, Minju ; Park, Sun Kyu ; Han, Hae Jung ; An, Subin ; Kang, Jong-Sun ; Lee, Sang-Jin ; Bae, Gyu-Un</creator><creatorcontrib>Kim, Ryuni ; Kim, Hyebeen ; Im, Minju ; Park, Sun Kyu ; Han, Hae Jung ; An, Subin ; Kang, Jong-Sun ; Lee, Sang-Jin ; Bae, Gyu-Un</creatorcontrib><description>BST204 is a purified ginseng dry extract that has an inhibitory effect on lipopolysaccharide-induced inflammatory responses, but its effect on muscle atrophy is yet to be investigated. In this study, C2C12 myoblasts were induced to differentiate for three days followed by the treatment of dexamethasone (DEX), a corticosteroid drug, with vehicle or BST204 for one day and subjected to immunoblotting, immunocytochemistry, qRT-PCR and biochemical analysis for mitochondrial function. BST204 alleviates the myotube atrophic effect mediated by DEX via the activation of protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling. Through this pathway, BST204 suppresses the expression of muscle-specific E3 ubiquitin ligases contributing to the enhanced myotube formation and enlarged myotube diameter in DEX-treated myotubes. In addition, BST204 treatment significantly decreases the mitochondrial reactive oxygen species production in DEX-treated myotubes. Furthermore, BST204 improves mitochondrial function by upregulating the expression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC1 alpha) in DEX-induced myotube atrophy. This study provides a mechanistic insight into the effect of BST204 on DEX-induced myotube atrophy, suggesting that BST204 has protective effects against the toxicity of a corticosteroid drug in muscle and promising potential as a nutraceutical remedy for the treatment of muscle weakness and atrophy.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph18052367</identifier><identifier>PMID: 33804338</identifier><language>eng</language><publisher>BASEL: Mdpi</publisher><subject>Dexamethasone - toxicity ; Environmental Sciences ; Environmental Sciences &amp; Ecology ; Humans ; Life Sciences &amp; Biomedicine ; Mitochondria ; Muscle Fibers, Skeletal - metabolism ; Muscle, Skeletal ; Muscular Atrophy - chemically induced ; Muscular Atrophy - drug therapy ; Muscular Atrophy - prevention &amp; control ; Public, Environmental &amp; Occupational Health ; Science &amp; Technology ; Up-Regulation</subject><ispartof>International journal of environmental research and public health, 2021-03, Vol.18 (5), p.2367, Article 2367</ispartof><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>7</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000628153800001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c390t-b176270e2604fb62147f39876072c3f15031e50bf0eedd736d6e9ef5d5143acf3</citedby><cites>FETCH-LOGICAL-c390t-b176270e2604fb62147f39876072c3f15031e50bf0eedd736d6e9ef5d5143acf3</cites><orcidid>0000-0002-4386-6934</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957540/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957540/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,39262,39263,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33804338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Ryuni</creatorcontrib><creatorcontrib>Kim, Hyebeen</creatorcontrib><creatorcontrib>Im, Minju</creatorcontrib><creatorcontrib>Park, Sun Kyu</creatorcontrib><creatorcontrib>Han, Hae Jung</creatorcontrib><creatorcontrib>An, Subin</creatorcontrib><creatorcontrib>Kang, Jong-Sun</creatorcontrib><creatorcontrib>Lee, Sang-Jin</creatorcontrib><creatorcontrib>Bae, Gyu-Un</creatorcontrib><title>BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function</title><title>International journal of environmental research and public health</title><addtitle>INT J ENV RES PUB HE</addtitle><addtitle>Int J Environ Res Public Health</addtitle><description>BST204 is a purified ginseng dry extract that has an inhibitory effect on lipopolysaccharide-induced inflammatory responses, but its effect on muscle atrophy is yet to be investigated. In this study, C2C12 myoblasts were induced to differentiate for three days followed by the treatment of dexamethasone (DEX), a corticosteroid drug, with vehicle or BST204 for one day and subjected to immunoblotting, immunocytochemistry, qRT-PCR and biochemical analysis for mitochondrial function. BST204 alleviates the myotube atrophic effect mediated by DEX via the activation of protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling. Through this pathway, BST204 suppresses the expression of muscle-specific E3 ubiquitin ligases contributing to the enhanced myotube formation and enlarged myotube diameter in DEX-treated myotubes. In addition, BST204 treatment significantly decreases the mitochondrial reactive oxygen species production in DEX-treated myotubes. Furthermore, BST204 improves mitochondrial function by upregulating the expression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC1 alpha) in DEX-induced myotube atrophy. This study provides a mechanistic insight into the effect of BST204 on DEX-induced myotube atrophy, suggesting that BST204 has protective effects against the toxicity of a corticosteroid drug in muscle and promising potential as a nutraceutical remedy for the treatment of muscle weakness and atrophy.</description><subject>Dexamethasone - toxicity</subject><subject>Environmental Sciences</subject><subject>Environmental Sciences &amp; Ecology</subject><subject>Humans</subject><subject>Life Sciences &amp; Biomedicine</subject><subject>Mitochondria</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle, Skeletal</subject><subject>Muscular Atrophy - chemically induced</subject><subject>Muscular Atrophy - drug therapy</subject><subject>Muscular Atrophy - prevention &amp; control</subject><subject>Public, Environmental &amp; Occupational Health</subject><subject>Science &amp; Technology</subject><subject>Up-Regulation</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>GIZIO</sourceid><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqNkdFP2zAQxq2JaRTY6x5R3lG6c5zYyQtS6eiGxLRJwHPkOOfGVWtHjgPrC387Zt0q-oYffNbd7_usuyPkC4UpYxV8NSv0fUdLKDLGxQcyoZxDmnOgR2_ex-RkGFYArMx59YkcM1ZCHq8Jeb66u88gT357F1CFIfmGf-QGQycHZzG9se2osE1-bl0YG0xmwbu-2yah825cdjFi8tB7XI5rGYyzidN7duH8ZpeUNjqY4FTnbOuNXCeL0arX0hn5qOV6wM__4il5WFzfz3-kt7--38xnt6mKTYa0oYJnAjDjkOuGZzQXmlWl4CAyxTQtgFEsoNGA2LaC8ZZjhbpoC5ozqTQ7JZc7335sNtgqtMHLdd17s5F-Wztp6sOKNV29dI-1qApR5BANpjsD5d0weNR7LYX6dRP14Sai4Pztj3v8_-gjUO6AJ2ycHpRBq3CPAQDPSlpEOh46N-HvKOdutCFKL94vZS-aeKmR</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Kim, Ryuni</creator><creator>Kim, Hyebeen</creator><creator>Im, Minju</creator><creator>Park, Sun Kyu</creator><creator>Han, Hae Jung</creator><creator>An, Subin</creator><creator>Kang, Jong-Sun</creator><creator>Lee, Sang-Jin</creator><creator>Bae, Gyu-Un</creator><general>Mdpi</general><general>MDPI</general><scope>17B</scope><scope>BLEPL</scope><scope>DTL</scope><scope>DVR</scope><scope>EGQ</scope><scope>GIZIO</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4386-6934</orcidid></search><sort><creationdate>20210301</creationdate><title>BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function</title><author>Kim, Ryuni ; Kim, Hyebeen ; Im, Minju ; Park, Sun Kyu ; Han, Hae Jung ; An, Subin ; Kang, Jong-Sun ; Lee, Sang-Jin ; Bae, Gyu-Un</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-b176270e2604fb62147f39876072c3f15031e50bf0eedd736d6e9ef5d5143acf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Dexamethasone - toxicity</topic><topic>Environmental Sciences</topic><topic>Environmental Sciences &amp; Ecology</topic><topic>Humans</topic><topic>Life Sciences &amp; Biomedicine</topic><topic>Mitochondria</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle, Skeletal</topic><topic>Muscular Atrophy - chemically induced</topic><topic>Muscular Atrophy - drug therapy</topic><topic>Muscular Atrophy - prevention &amp; control</topic><topic>Public, Environmental &amp; Occupational Health</topic><topic>Science &amp; Technology</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Ryuni</creatorcontrib><creatorcontrib>Kim, Hyebeen</creatorcontrib><creatorcontrib>Im, Minju</creatorcontrib><creatorcontrib>Park, Sun Kyu</creatorcontrib><creatorcontrib>Han, Hae Jung</creatorcontrib><creatorcontrib>An, Subin</creatorcontrib><creatorcontrib>Kang, Jong-Sun</creatorcontrib><creatorcontrib>Lee, Sang-Jin</creatorcontrib><creatorcontrib>Bae, Gyu-Un</creatorcontrib><collection>Web of Knowledge</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Social Sciences Citation Index</collection><collection>Web of Science Primary (SCIE, SSCI &amp; AHCI)</collection><collection>Web of Science - Social Sciences Citation Index – 2021</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Ryuni</au><au>Kim, Hyebeen</au><au>Im, Minju</au><au>Park, Sun Kyu</au><au>Han, Hae Jung</au><au>An, Subin</au><au>Kang, Jong-Sun</au><au>Lee, Sang-Jin</au><au>Bae, Gyu-Un</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function</atitle><jtitle>International journal of environmental research and public health</jtitle><stitle>INT J ENV RES PUB HE</stitle><addtitle>Int J Environ Res Public Health</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>18</volume><issue>5</issue><spage>2367</spage><pages>2367-</pages><artnum>2367</artnum><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>BST204 is a purified ginseng dry extract that has an inhibitory effect on lipopolysaccharide-induced inflammatory responses, but its effect on muscle atrophy is yet to be investigated. In this study, C2C12 myoblasts were induced to differentiate for three days followed by the treatment of dexamethasone (DEX), a corticosteroid drug, with vehicle or BST204 for one day and subjected to immunoblotting, immunocytochemistry, qRT-PCR and biochemical analysis for mitochondrial function. BST204 alleviates the myotube atrophic effect mediated by DEX via the activation of protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling. Through this pathway, BST204 suppresses the expression of muscle-specific E3 ubiquitin ligases contributing to the enhanced myotube formation and enlarged myotube diameter in DEX-treated myotubes. In addition, BST204 treatment significantly decreases the mitochondrial reactive oxygen species production in DEX-treated myotubes. Furthermore, BST204 improves mitochondrial function by upregulating the expression of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC1 alpha) in DEX-induced myotube atrophy. This study provides a mechanistic insight into the effect of BST204 on DEX-induced myotube atrophy, suggesting that BST204 has protective effects against the toxicity of a corticosteroid drug in muscle and promising potential as a nutraceutical remedy for the treatment of muscle weakness and atrophy.</abstract><cop>BASEL</cop><pub>Mdpi</pub><pmid>33804338</pmid><doi>10.3390/ijerph18052367</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4386-6934</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1660-4601
ispartof International journal of environmental research and public health, 2021-03, Vol.18 (5), p.2367, Article 2367
issn 1660-4601
1661-7827
1660-4601
language eng
recordid cdi_crossref_primary_10_3390_ijerph18052367
source MEDLINE; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; EZB-FREE-00999 freely available EZB journals; PubMed Central; Web of Science - Social Sciences Citation Index – 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" />; Free Full-Text Journals in Chemistry
subjects Dexamethasone - toxicity
Environmental Sciences
Environmental Sciences & Ecology
Humans
Life Sciences & Biomedicine
Mitochondria
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal
Muscular Atrophy - chemically induced
Muscular Atrophy - drug therapy
Muscular Atrophy - prevention & control
Public, Environmental & Occupational Health
Science & Technology
Up-Regulation
title BST204 Protects Dexamethasone-Induced Myotube Atrophy through the Upregulation of Myotube Formation and Mitochondrial Function
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T02%3A37%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=BST204%20Protects%20Dexamethasone-Induced%20Myotube%20Atrophy%20through%20the%20Upregulation%20of%20Myotube%20Formation%20and%20Mitochondrial%20Function&rft.jtitle=International%20journal%20of%20environmental%20research%20and%20public%20health&rft.au=Kim,%20Ryuni&rft.date=2021-03-01&rft.volume=18&rft.issue=5&rft.spage=2367&rft.pages=2367-&rft.artnum=2367&rft.issn=1660-4601&rft.eissn=1660-4601&rft_id=info:doi/10.3390/ijerph18052367&rft_dat=%3Cpubmed_cross%3E33804338%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/33804338&rfr_iscdi=true